Paneth Cell Defects Induce Microbiota Dysbiosis in Mice and Promote Visceral Hypersensitivity

Riba, Ambre; Olier, Maı̈wenn; Lacroix‐Lamandé, Sonia; Lencina, Corinne; Bacquié, Valerie; Harkat, Cherryl; Gillet, M.; Baron, Marine; Sommer, Caroline; Mallet, Virginie; Salvador–Cartier, Christel; Laurent, Fabrice; Théodorou, Vassilia; Ménard, Sandrine

doi:10.1053/j.gastro.2017.08.044

Cited by 67 publications

(51 citation statements)

References 54 publications

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“…In rodent models, the abnormal gut motor pattern was also documented several years ago . Indeed, an acceleration of gastrointestinal transit was shown using the restraint stress model and confirmed later by others using other models of stress (water avoidance stress, early life trauma) or by CRF administration mimicking the stress effects . In this study, the stress‐induced acceleration of the gut transit was significantly reduced by SP‐11.…”

Section: Discussionsupporting

confidence: 83%

The multicomponent medication Spascupreel attenuates stress‐induced gut dysfunction in rats

Théodorou

Beaufrand

Yvon

et al. 2020

Neurogastroenterology Motil

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Background: Irritable bowel syndrome (IBS) is a common disorder worldwide. It is characterized by abdominal pain/discomfort and changes in bowel habits. Due to themultifactorialpathophysiologyandtheheterogeneityofIBSpatients,appropriate treatment of IBS is still a challenge. Spascupreel (SP-11), as a multicomponent medication,hasthepotentialtomodulatemultiplepathophysiologicalpathwayssimultaneously.Therefore,theobjectiveofthecurrentstudywastoinvestigatethe effectsoforalSP-11treatmentonstress-inducedchangesofperipheralandcentral functionsinaratmodelmimickinghumanIBS. Methods: NaïveWistarratsweretreatedwithSP-11(0.9tab/kg)orNaCl0.9%by oralgavagefor4daysbefore2-hourpartialrestraintstress(PRS)procedure.Twenty minutesafterPRS,centralandperipheralstress-inducedchangesaffectingIBSwere assessed. These include the hypothalamic-pituitary-adrenal (HPA) axis response throughplasmaACTHandcorticosteronemeasurements,visceralpaininresponse tocolorectaldistension,gutpermeability,colonicmastcellnumber,andsensitization as well as gut transit time. Results: TreatmentwithSP-11reducedtheHPAaxisactivationinresponsetoPRS. At the gut level, a reduction in colonic hypersensitivity to colorectal distension, a normalizationofguttransittimeacceleration,areducedmastcellsensitization,anda trend toward reduced gut hyperpermeability were observed. Conclusions: Thesedatasuggestthatstress-inducedIBSsignscanbereducedusing SP-11inrats.TheobservedeffectsandthegoodtolerabilityofthedrugmakeSP-11 aninnovativecandidateinthemanagementofIBS. K E Y W O R D S gut-brainaxis,irritablebowelsyndrome,multicomponentmedication,partialrestraintstress, Spascupreel,SP-11 How to cite this article:TheodorouV,BeaufrandC,YvonS,et al. The multicomponent medication Spascupreel attenuates stress-inducedgutdysfunctioninrats.Neurogastroenterol

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Section: Discussionsupporting

confidence: 83%

The multicomponent medication Spascupreel attenuates stress‐induced gut dysfunction in rats

Théodorou

Beaufrand

Yvon

et al. 2020

Neurogastroenterology Motil

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“…We present evidence that H19 regulates intestinal barrier function partially by modulating the levels of Paneth and goblet cells that are responsible for mucosal defense and host protection from enteric pathogens. 41,42 Paneth and goblet cells increased in the absence of H19 in vivo as well as ex vivo, and H19 knockout also prevented CLP-induced inhibition of Paneth and goblet cells and improved intestinal barrier function. In an ex vivo model, H19-deficient enteroids from H19 -/mice showed a protective effect on Paneth and goblet cells against LPS.…”

Section: Discussionmentioning

confidence: 89%

Long Noncoding RNA H19 Impairs the Intestinal Barrier by Suppressing Autophagy and Lowering Paneth and Goblet Cell Function

Chung

Xiao

et al. 2020

Cellular and Molecular Gastroenterology and Hepatology

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“…Paneth and goblet cells are indispensable for maintaining homeostasis with enteric microbes (Baurhoo et al, ; Vaishnava et al, ) as they promote the removal of microbes from the mucosal surface (Chairatana and Nolan, ). Reduction in number or defects in activity of these cells lead to microbiota disbiosis (Baurhoo et al, ; Riba et al, ). Our data showed significant changes in the gut microbiota only in rats from PS group with substantial depletion of Paneth and goblet cells.…”

Section: Discussionmentioning

confidence: 99%

Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats

et al. 2019

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Aims To evaluate efficacy of Saccharomyces cerevisiae fermentate prebiotic (EH) in protection of intestinal barrier integrity in rats during heat stress, to analyze the impact of heat stress and preventive treatment with EH on the structure of the gut microbiota. Methods and Results Two groups of rats were treated orally with EH or phosphate‐buffered saline for 14 days. On day 15, half of the rats in each group were exposed to heat stress conditions, while control animals were kept at room temperature. Histological and Western blot analyses of the intestine, culture‐based microbiological analysis and high‐throughput 16S rRNA sequencing for the gut microbiota were performed for each rat. Exposure of animals to heat stress conditions resulted in inhibition of tight junction (TJ) proteins expression, decrease of Paneth and goblet cells, decrease of beneficial and increase of pathogenic bacteria. Oral treatment of rats with EH before stress significantly prevents these adverse effects by elevation of the gut beneficial bacteria, particularly butyrate‐producing bacteria. Conclusions Essential effect of EH in protection of intestinal barrier integrity during heat stress is connected with beneficial modulation of the gut microbiota. Significance and Impact of the Study Our results will contribute to the development of new approaches to prevention of heat stress‐related complications.

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Paneth Cell Defects Induce Microbiota Dysbiosis in Mice and Promote Visceral Hypersensitivity

Abstract: Mice with defects in Paneth cells (induced by maternal separation or genetically engineered) have intestinal expansion of E coli leading to visceral hypersensitivity. These findings provide evidence that Paneth cell function and intestinal dysbiosis are involved in visceral sensitivity.

Cited by 67 publications

References 54 publications

The multicomponent medication Spascupreel attenuates stress‐induced gut dysfunction in rats

The multicomponent medication Spascupreel attenuates stress‐induced gut dysfunction in rats

Long Noncoding RNA H19 Impairs the Intestinal Barrier by Suppressing Autophagy and Lowering Paneth and Goblet Cell Function

Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats

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