Pangenomic and functional investigations for dormancy and biodegradation features of an organic pollutant-degrading bacterium Rhodococcus biphenylivorans TG9

Rhodococcus strains as a good biotool for neutralizing pharmaceutical pollutants and obtaining therapeutically valuable products: Through the past into the future

“…It is well known that many bacteria can withstand stressors such as cold, desiccation, and xenobiotics by going into dormant (metabolically inactive) states. Resuscitation of degraders in the dormant state may accelerate the rate of xenobiotic degradation in the environment (Yu et al, 2022b). Compared with vegetative cells, reactivated cyst-like dormant cells of R. ruber IEGM 346 demonstrated increased degrading activity towards drotaverine as the only carbon and energy source and under co-oxidation (glucose), including suboptimal (35 ± 2°C) growth conditions (Ivshina et al, 2015).…”

“…It is well known that many bacteria can withstand stressors such as cold, desiccation, and xenobiotics by going into dormant (metabolically inactive) states. Resuscitation of degraders in the dormant state may accelerate the rate of xenobiotic degradation in the environment ( Yu et al, 2022b ). Compared with vegetative cells, reactivated cyst-like dormant cells of R .…”

Antibiotic tolerance and degradation capacity of the organic pollutant-degrading bacterium Rhodococcus biphenylivorans TG9T

“…Our previous work revealed that R. biphenylivorans TG9 T could use at least 14 pollutants as its sole carbon source, and genes involved in 20 xenobiotics biodegradation and metabolism pathways were annotated in its genome (Yu et al, 2021). Regarding the proteomic analysis, we observed differential abundance of proteins in the three states related to 14 pathways of xenobiotics biodegradation and metabolism, including the degradation of chlorocyclohexane and chlorobenzene, benzoate, fluorobenzoate, xylene, dioxin, toluene, naphthalene, atrazine, aminobenzoate, styrene, steroid, polycyclic aromatic hydrocarbons, chloroalkane and chloroalkene, and caprolactam (Supplementary Fig.…”

“…R. biphenylivorans TG9 T is a Grampositive, aerobic, and rod-coccus shaped actinobacteria. The strain can use at least 14 types of organic pollutants as sole source of carbon/energy, notably including biphenyl and polychlorinated biphenyl (PCB) (Yu et al, 2021). It was originally isolated from organic pollutant-contaminated sediment following recovery from the dormant state via exposure to resuscitation-promoting factor (Rpf) protein (Su et al, 2015).…”

“…It was originally isolated from organic pollutant-contaminated sediment following recovery from the dormant state via exposure to resuscitation-promoting factor (Rpf) protein (Su et al, 2015). The genome of TG9 T has many predicted antibiotic resistance genes (i.e., TG9 T is resistant to gentamicin and streptomycin, rifampin, tetracycline, chloramphenicol, and polymyxin as presented by Yu et all., (Yu et al, 2021) ), indicating the potential for survival in antibiotic-contaminated environments. However, if instead of adopting a resistance phenotype, the strain were to switch to tolerance, it would enter dormancy to survive and only resuscitate when the conditions become favorable.…”