Panning using a phage-displayed random peptide library to identify peptides that antagonize the Helicobacter pylori ArsS acid-sensing domain

Yang, Xiong; Yang, Zhibang; Zhang, Jin; Li, Jinyang; Chen, Pu; Xiang, Yu

doi:10.1016/j.micpath.2019.103614

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…The diagnostic potential of these antibodies was demonstrated by the high affinity of their complementarity determining regions (CDR) toward VacA toxin [88]. In addition, a similar investigation has developed ArsS antagonistic peptides that exhibit the ability to obstruct acid-sensing signal transduction, thereby presenting an unfavorable condition for H. pylori proliferation [89]. Facchin and colleagues obtained peptides from patients diagnosed with ulcerative colitis and Crohn's.…”

Section: Gastrointestinal Disordersmentioning

confidence: 99%

Phage Display Technology in Biomarker Identification with Emphasis on Non-Cancerous Diseases

Sadraeian,

Maleki,

Moraghebi

et al. 2024

Molecules

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In recent years, phage display technology has become vital in clinical research. It helps create antibodies that can specifically bind to complex antigens, which is crucial for identifying biomarkers and improving diagnostics and treatments. However, existing reviews often overlook its importance in areas outside cancer research. This review aims to fill that gap by explaining the basics of phage display and its applications in detecting and treating various non-cancerous diseases. We focus especially on its role in degenerative diseases, inflammatory and autoimmune diseases, and chronic non-communicable diseases, showing how it is changing the way we diagnose and treat illnesses. By highlighting important discoveries and future possibilities, we hope to emphasize the significance of phage display in modern healthcare.

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Section: Gastrointestinal Disordersmentioning

confidence: 99%

Phage Display Technology in Biomarker Identification with Emphasis on Non-Cancerous Diseases

Sadraeian,

Maleki,

Moraghebi

et al. 2024

Molecules

View full text Add to dashboard Cite

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“…For instance, in the Epsilonproteobacterium H. pylori, which has a three-tiered regulatory hierarchy of flagellar expression and assembly, including three sigma factors and a FlgM feedback loop (Niehus et al, 2004), researchers have found that several flagellar structural proteins are involved in flagellar regulation. These proteins include the basal body component FlhA, an essential part of the basal body and the assembly platform (Schmitz et al, 1997;Rust et al, 2009;Minamino et al, 2016a,b;Terahara et al, 2018) as well as FlhF, considered a signal recognition particle (SRP)like protein important for flagellar localization (Bange et al, 2007;Kazmierczak and Hendrixson, 2013;Kondo et al, 2017); additionally the TCSs FlgS-FlgR and ArsS-ArsR are involved (Pflock et al, 2006;Loh et al, 2010;Marcus et al, 2016;Xiong et al, 2019).…”

Section: Protein-protein Interaction Sensing In Flagellar Assembly Rementioning

confidence: 99%

Protein Activity Sensing in Bacteria in Regulating Metabolism and Motility

2020

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Bacteria have evolved complex sensing and signaling systems to react to their changing environments, most of which are present in all domains of life. Canonical bacterial sensing and signaling modules, such as membrane-bound ligand-binding receptors and kinases, are very well described. However, there are distinct sensing mechanisms in bacteria that are less studied. For instance, the sensing of internal or external cues can also be mediated by changes in protein conformation, which can either be implicated in enzymatic reactions, transport channel formation or other important cellular functions. These activities can then feed into pathways of characterized kinases, which translocate the information to the DNA or other response units. This type of bacterial sensory activity has previously been termed protein activity sensing. In this review, we highlight the recent findings about this non-canonical sensory mechanism, as well as its involvement in metabolic functions and bacterial motility. Additionally, we explore some of the specific proteins and protein-protein interactions that mediate protein activity sensing and their downstream effects. The complex sensory activities covered in this review are important for bacterial navigation and gene regulation in their dynamic environment, be it host-associated, in microbial communities or free-living.

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“…The complementarity determining regions (CDR) of these antibodies showed high affinity against VacA toxin, indicating its application as diagnostic tool [34]. Similarly, a recent study has reported ArsS antagonistic peptides that are capable of blocking acid-sensing signals, ultimately, providing a challenging environment for the growth of h. pylori [35]. Facchin, et al [36] extracted peptides from ulcerative colitis and Crohn's disease patients which were integrated into virusmimicking nanoassemblies, composed of 400 peptides per nanoparticle.…”

Section: Gastrointestinal Disordersmentioning

confidence: 99%

Clinical implications of phage display technique: Digging deeper in diagnostic and therapeutic grounds.

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Phage display is considered as a gold-standard technique for the extraction of monoclonal antibodies. Within the past decade, the technique has been extensively applied in clinical studies for the discovery of peptides, ligands and antibodies that can be implemented for the detection of clinical biomarkers and therapeutic purposes. This review highlights recent advancements in the field of phage display technology that has aided medicine in therapeutic and diagnostic terms.

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Panning using a phage-displayed random peptide library to identify peptides that antagonize the Helicobacter pylori ArsS acid-sensing domain

Cited by 5 publications

References 28 publications

Phage Display Technology in Biomarker Identification with Emphasis on Non-Cancerous Diseases

Phage Display Technology in Biomarker Identification with Emphasis on Non-Cancerous Diseases

Protein Activity Sensing in Bacteria in Regulating Metabolism and Motility

Clinical implications of phage display technique: Digging deeper in diagnostic and therapeutic grounds.

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