Panos-Fermented Extract-Mediated Nanoemulsion: Preparation, Characterization, and In Vitro Anti-Inflammatory Effects on RAW 264.7 Cells

Zhang, Rui; Rupa, Esrat Jahan; Zheng, Siwen; Nahar, Jinnatun; Yang, Deok‐Chun; Kang, Se Chan; Wang, Ying‐Ping

doi:10.3390/molecules27010218

Cited by 3 publications

(1 citation statement)

References 33 publications

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“…The study revealed that Panax ginseng, which is known as the king of medicinal plants, successfully formed NE with sea buckwheat fruit oil. That study also demonstrated the inhibition of pro-inflammatory mediators in vitro compared to Panax ginseng alone [79,80]. Previously, an in vivo study in a mouse ear model with curcumin NE (formed with MCT) from the Curcuma longa medicinal plant focused on the inhibition effects of edema as an anti-inflammatory potential [81].…”

Section: Plant-based Nanoemulsion For Inflammationmentioning

confidence: 73%

Medicinal Plant Enriched Metal Nanoparticles and Nanoemulsion for Inflammation Treatment: A Narrative Review on Current Status and Future Perspective

Puja

Rupa

Kim

et al. 2023

Immuno

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Inflammation is considered a natural reaction of the immune system that can be caused by several factors such as pathogens, chemical substances, and damaged cells. Since the classical era, therapeutic substances have been made from medicinal plants. According to recent studies, nanotechnology provides a fresh approach to maintaining the standard quality, distribution, and bioactivity of therapeutic compounds. This review emphasizes the anti-inflammatory effects of green, synthetic, plant-based nanoparticles and nanoemulsions. A reduction of the dosage of anti-inflammatory medications and an improved therapeutic impact is highly desirable with an efficient drug delivery method. Along with the discussion of nanotechnology of medicinal plant-based anti-inflammatory effects, this review also offers a perspective view of the use of nanoparticles and nanoemulsions in inflammatory diseases in the future.

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Section: Plant-based Nanoemulsion For Inflammationmentioning

confidence: 73%

Medicinal Plant Enriched Metal Nanoparticles and Nanoemulsion for Inflammation Treatment: A Narrative Review on Current Status and Future Perspective

Puja

Rupa

Kim

et al. 2023

Immuno

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Nanoemulsions of Bioactive Lipids Isolated from Cladophora Green Algae Declined NO and IL-6 Production in LPS-simulated RAW264.7 Murine Macrophage Cells

Foroghi,

Sarafbidabad,

Nasiri

2024

Nano LIFE

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Inflammation is part of the host’s normal response to various harmful stimuli, including chemical exposure, tissue damage, infection, or contact with bacterial components, such as lipopolysaccharide (LPS); however, excessive inflammation leads to various acute and chronic human diseases. Macrophages contribute to the inflammatory process by secreting inflammatory mediators, such as prostaglandin E2 (PGE2) and nitric oxide (NO), as well as proinflammatory cytokines such as IL-1[Formula: see text], TNF-[Formula: see text] and IL-6 and the excess of these mediators can be harmful to tissues and organisms. The RAW 264.7 murine macrophage cell line treated with LPS is a commonly used in vitro model for assessing anti-inflammatory efficacy. The base of conventional inflammation therapy is the use of steroidal and nonsteroidal anti-inflammatory medications, both of which have significant adverse effects. Therefore, finding new sources of less hazardous treatments is imperative. The anti-inflammatory qualities of bioactive lipids from crude extracts of the green alga Cladophora were investigated using ethanol and hexane-ether as two distinct solvents. As a result, the total lipid concentration of the hexane-ether extraction was higher, so the unsaturated fatty acids (UFAs) were extracted from this sample by the methanol-solvent crystallization technique at low temperatures. The presence of various fatty acids and their percentage purity were determined by gas chromatography (GC). After the elimination of 49.11% of the saturated fatty acids, the spontaneous nanoemulsification approach was applied to improve the stability and functionalization of the fatty acids. The nanoemulsion particles’ size, distribution, and stability were evaluated using a Zetasizer system. The mean particle size and zeta potential value were 194.3 nm and −25.8 mV, respectively. Finally, the MTT (thiazolyl blue tetrazolium bromide) assay was used to determine the toxicity of the extracted bioactive lipids in the macrophage cell line RAW264.7 for 24 h, and the results showed an increase in the viability of cells treated with UFAs after encapsulation. The IC[Formula: see text] values calculated from the MTT assay results for each formulation and the anti-inflammatory effects of each formulation (at 1/3 IC[Formula: see text] value) were determined in LPS-stimulated RAW 264.7 macrophages by performing nitric oxide (NO) and cytokine enzyme-linked immunosorbent assays. Nanoemulsions of polyunsaturated fatty acids inhibited the production of NO and interleukin 6, which may be promising candidates for biological and medical applications to combat inflammation.

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Discovery of Anti-Inflammatory Triterpenoid Glucosides from the Heritiera littoralis Dryand

Liang

Niu

et al. 2023

Molecules

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Two new triterpenoid glucosides, Heritiera A (1) and Heritiera B (2), and six known triterpenoid analogs (3–8) were isolated from Heritiera littoralis Dryand. Their structures were identified by comprehensive spectroscopic analyses and comparisons with the literature. The anti-inflammatory activity of the isolates from H. littoralis was evaluated using a lipopolysaccharide (LPS) stimulated RAW 264.7 cells model. The result showed that four triterpenoids exhibited potent anti-inflammatory activity. Among these compounds, compound 2 substantially inhibits the release of nitric oxide (NO) with an IC50 value of 10.33 μM. The triterpenoids from H. littoralis could be used as potential candidates for the development of new anti-inflammatory agents.

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Panos-Fermented Extract-Mediated Nanoemulsion: Preparation, Characterization, and In Vitro Anti-Inflammatory Effects on RAW 264.7 Cells

Cited by 3 publications

References 33 publications

Medicinal Plant Enriched Metal Nanoparticles and Nanoemulsion for Inflammation Treatment: A Narrative Review on Current Status and Future Perspective

Medicinal Plant Enriched Metal Nanoparticles and Nanoemulsion for Inflammation Treatment: A Narrative Review on Current Status and Future Perspective

Nanoemulsions of Bioactive Lipids Isolated from Cladophora Green Algae Declined NO and IL-6 Production in LPS-simulated RAW264.7 Murine Macrophage Cells

Discovery of Anti-Inflammatory Triterpenoid Glucosides from the Heritiera littoralis Dryand

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