Pantoea rodasii sp. nov., Pantoea rwandensis sp. nov. and Pantoea wallisii sp. nov., isolated from Eucalyptus

Abstract

Pantoea ananatis has been reported as the causal agent of bacterial blight and dieback of Eucalyptus seedlings in South Africa. Young leaves present symptoms first, with leaf spots that become water-soaked and eventually form larger necrotic lesions. Trees either fail to survive or become multi-stemmed (Coutinho et al., 2002). In the last decade, similar symptoms have been observed in nurseries and plantations in Uganda, Argentina and Uruguay. The bacteria isolated from these diseased trees were identified as belonging to three novel species of the genus Pantoea: P. vagans, P. eucalypti and P. deleyi (Brady et al., 2009). It has been suggested that a complex of Pantoea species may be responsible for bacterial blight and dieback in Africa and South America (Coutinho et al., 2011). P. ananatis, P. vagans, P. eucalypti and P. deleyi, have been isolated from a wide range of Eucalyptus species, hybrids and clones which is of concern for the forestry industry.As part of an on-going isolation campaign in countries of Africa, South America and Asia, Eucalyptus seedlings are regularly examined for symptoms of bacterial blight and dieback. Bacterial isolates obtained from the diseased material are identified using a polyphasic approach based on Gram staining, oxidation-fermentation testing, partial 16S rRNA gene-and gyrB-sequencing. P. ananatis and P. eucalypti are regularly isolated in South Africa, while P. vagans and P. dispersa are more commonly isolated in Colombia and Thailand, respectively (Swart, 2009). In 2006/2007, bacteria were isolated from diseased Eucalyptus material in Colombia, Rwanda and South Africa which could not be assigned to any of the recognized species of the genus Pantoea. Sequencing of the gyrB gene placed these isolates in the genus Pantoea, and indicated that they constituted three novel species (Swart, 2009). In the present study, these isolates were further examined using a polyphasic approach to confirm that they constitute three novel species of the genus Pantoea.Bacteria were isolated from diseased Eucalyptus material as previously described (Brady et al., 2009). Reference strains were obtained from the BCCM/LMG Bacteria Collection (http://bccm.belspo.be) and recovered on tryptic soy agar according to the provider's instructions. A list of strains Abbreviation: MLSA, multilocus sequence analysis.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, atpD, gyrB, infB and rpoB gene sequences

Pantoea theicola sp. nov., isolated from black tea

“…The phenotypic characteristics of strain QC88-366 T generally corresponded to the emended description of the genus Brady et al (2008Brady et al ( , 2009Brady et al ( , 2010Brady et al ( , 2011Brady et al ( , 2012 and Popp et al (2010). Table 1.…”

“…indologenes. Data were taken from the following sources: taxa 1-3 from this study; taxa 4-8 from Brady et al (2010); taxa 9-11 from Brady et al (2012); taxa 12-15 from Brady et al (2009); taxa 16 and 17 are type strain data from Gavini et al (1989) and Grimont & Grimont (2005); taxon 18 from Brady et al (2011); taxa 19-21 are type strains data from Mergaert et al (1993).+, 90-100 % strains positive in 1-2 days; (+), slow reaction (between 2 and 30 days); 2, negative; D, 11-89 % strains positive in 1-4 days; ND, not determined. Citrate utilization 2 + + D + + + + 2 2 + 2 2 ND 2 + + + + 2 + Indol production 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 + + 2 + Acetoin production 2 + + ND ND ND ND ND + ND ND + + + + + + + + 2 + Gelatinase 2 2 + ND ND ND ND ND ND ND ND 2 2 ND 2 + + 2 2 2 2 Acid production from:…”

Gibbsiella dentisursi sp. nov., isolated from the bear oral cavity

“…The DNA G + C content of strain NUM 1720 T was 55.0 mol%. This value is slightly lower than those of the genus Gibbsiella (56.0–56.4 mol%) (1), S. ficaria (59.6 mol%) (13) and K. ascorbata (56.1 mol%) (14), and slightly higher than that of P. rwandensis (51.2 mol%) (15).…”

Discovery of Pantoea rodasii Strain ND03 that Produces N-(3-Oxo-hexanoyl)-L-homoserine Lactone

“…According to research done by Brady and colleagues, Pantoea rodasii is a novel species which is closest related to Pantoea dispersa, Pantoea eucrina and Pantoea cypripedii based on partial sequences of gyrB, rpoB, infB and atpD using multilocus sequence analysis (MLSA) [24]. Hence, there is limited information about QS of these bacteria species.…”