Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia)

Bateson, M. F.; Jm, Henderson; Chaleeprom, Worawan; Gibbs, Adrian; Dale, James L.

doi:10.1099/0022-1317-75-12-3547

Cited by 94 publications

(72 citation statements)

References 28 publications

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“…The grouping of Indian isolates with American isolates (Brazil, Mexico, and Hawaii) and the level of divergence seen in the CP gene among the Indian isolates (Jain et al 1998;Bag et al 2007) certainly implicate the Indian subcontinent in the spread of PRSV to these countries. Close sequence similarity between the P and W biotypes of PRSV from the same geographic region suggests that they may have evolved by mutations (Bateson et al 1994). The sequence similarity matrix generated from whole genome sequences of PRSV isolates reported from different geographic locations and their individual genetic components revealed that all the cistrons follow the divergence exhibited by the whole genome, except P1, which is much more variable than the full genome (Inoue-Nagata et al 2007).…”

Section: Discussionmentioning

confidence: 97%

Role of Genetic Recombination in the Molecular Architecture of Papaya ringspot virus

et al. 2008

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Papaya ringspot virus (PRSV) has a single-stranded RNA genome and causes severe economic losses both in cucurbits and papaya worldwide. The extent to which the genome of PRSV is shaped by recombination provides an understanding of the molecular evolution of PRSV and helps in studying features such as host specificity, geographic distribution, and its emergence as new epidemics. The PRSV-P-Indian isolate was completely sequenced and compared with 14 other isolates reported from the rest of the world for their phylogenetic survey of recombination events. Cistron-by-cistron sequence comparison and phylogenetic analysis based on full-genome polyprotein showed two distinct groupings of Asian and American isolates, although PRSV-P and W-India clustered along with the American isolates. Recombination sites were found throughout the genomes, except in the small 6K1 protein gene. A significant proportion of recombination hotspots was found in the P1 gene, followed by P3, cylindrical inclusion (CI), and helper component proteinase (HcPro). Correlations between the presence of recombination sites, geographic distribution, and phylogenetic relationship provide an opportunity to establish the molecular evolution and geographic route of PRSV.

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Section: Discussionmentioning

confidence: 97%

Role of Genetic Recombination in the Molecular Architecture of Papaya ringspot virus

et al. 2008

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“…The cp gene of PRSV could be divided in three regions, N terminus (first 224 nucleotides), C terminus (last 59 nucleotides, including the stop codon), and core (641 nucleotides between the N and the C terminus), as suggested for other potyvirus (Shukla & Ward, 1989;Bateson et al, 1994). The analysis of the divergence between PRSV HA 5-1 and the Brazilian isolate throughout these three regions revealed that divergence is concentrated in the N terminus (21 nucleotides different) and core (36 nucleotides different) regions; nucleotide differences were not seen in the C terminus region.…”

Section: Resultsmentioning

confidence: 99%

Sequence similarity between the viral cp gene and the transgene in transgenic papayas

Júnior

2005

Pesq. agropec. bras.

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-The Papaya ringspot virus (PRSV) coat protein transgene present in 'Rainbow' and 'SunUp' papayas disclose high sequence similarity (>89%) to the cp gene from PRSV BR and TH. Despite this, both isolates are able to break down the resistance in 'Rainbow', while only the latter is able to do so in 'SunUp'. The objective of this work was to evaluate the degree of sequence similarity between the cp gene in the challenge isolate and the cp transgene in transgenic papayas resistant to PRSV. The production of a hybrid virus containing the genome backbone of PRSV HA up to the Apa I site in the NIb gene, and downstream from there, the sequence of PRSV TH was undertaken. This hybrid virus, PRSV HA/TH, was obtained and used to challenge 'Rainbow', 'SunUp', and an R2 population derived from line 63-1, all resistant to PRSV HA. PRSV HA/TH broke down the resistance in both papaya varieties and in the 63-1 population, demonstrating that sequence similarity is a major factor in the mechanism of resistance used by transgenic papayas expressing the cp gene. A comparative analysis of the cp gene present in line 55-1 and 63-1-derived transgenic plants and in PRSV HA, BR, and TH was also performed.Index terms: Carica papaya, potyvirus, resistance, breeding, virus. Similaridade de seqüência entre o gene cp do vírus e do transgene presente em mamoeiros transgênicosResumo -O gene da capa protéica (cp) do vírus da mancha anelar do mamoeiro (Papaya ringspot virus, PRSV), presente nos mamoeiros 'Rainbow' e 'SunUp', tem alta similaridade de seqüência (>89%) com o gene cp dos isolados PRSV BR e TH. Apesar deste alto grau de similaridade, ambos isolados são capazes de quebrar a resistência observada em 'Rainbow', ao passo que TH quebra a resistência em 'SunUp'. O objetivo deste trabalho foi avaliar o grau de similaridade de seqüência entre o gene cp do vírus desafiante e do transgene em mamoeiros transgênicos resistentes a PRSV. Produziu-se um vírus híbrido contendo o genoma do isolado PRSV HA até o sítio de restrição Apa I no gene NIb, e, a partir deste ponto, este vírus continha o genoma do isolado PRSV TH. PRSV HA/TH foi utilizado para desafiar plantas de 'Rainbow', 'SunUp' e de uma população R2 derivada da linha 63-1, todas resistentes a PRSV HA. PRSV HA/TH quebrou a resistência em todas essas plantas, demonstrando que a similaridade da seqüência é um fator preponderante no mecanismo de resistência utilizado pelos mamoeiros transgênicos expressando o gene cp de PRSV. Também foi realizada análise comparativa do transgene cp das plantas derivadas das linhas transgênicas 55-1 e 63-1, e do gene cp dos isolados PRSV HA, BR e TH.

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“…The sequence diversity of the isolates in the country as well as the surrounding countries would need to be known. The coat protein genes of many PRSV isolates in the world have been sequenced (e.g., BATESON et al 1994;WANG and YEH 1997). 9 Final Comments: an Interface Between "Innate" and "Acquired" Immunity…”

Section: Implementation Of Transgenic Papaya Throughout the Worldmentioning

confidence: 99%

Coat Protein Transgenic Papaya: “Acquired” Immunity for Controlling Papaya Ringspot Virus

Gonsalves

2002

The Interface Between Innate and Acquired Immunity

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Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia)

Cited by 94 publications

References 28 publications

Role of Genetic Recombination in the Molecular Architecture of Papaya ringspot virus

Role of Genetic Recombination in the Molecular Architecture of Papaya ringspot virus

Sequence similarity between the viral cp gene and the transgene in transgenic papayas

Coat Protein Transgenic Papaya: “Acquired” Immunity for Controlling Papaya Ringspot Virus

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