Paper-based ion concentration polarization device for selective preconcentration of muc1 and lamp-2 genes

Son, Seok Young; Lee, Hyomin; Kim, Sung Jae

doi:10.1186/s40486-017-0042-1

Cited by 17 publications

(8 citation statements)

References 17 publications

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“…A simple device design composed of a 10‐mm straight paper strip cut using an electronic craft cutter was reported for enrichment of two gene fragments, namely; muc1 (945 bp) a breast cancer marker and lamp‐2 (185 bp) a marker for Danon disease . The NMI was created by pipetting Nafion (0.5 μL) in the middle of the paper strip.…”

Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2016–2018)

et al. 2018

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One of the most cited limitations of capillary and microchip electrophoresis is the poor sensitivity. This review continues to update this series of biannual reviews, first published in Electrophoresis in 2007, on developments in the field of online/in‐line concentration methods in capillaries and microchips, covering the period July 2016–June 2018. It includes developments in the field of stacking, covering all methods from field‐amplified sample stacking and large‐volume sample stacking, through to isotachophoresis, dynamic pH junction, and sweeping. Attention is also given to online or in‐line extraction methods that have been used for electrophoresis.

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Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2016–2018)

et al. 2018

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“…[6][7][8][9] The electric field strength within the ion depleted zone (IDZ) is ohmically enhanced, and an extended electric field gradient forms along the IDZ boundary. This gradient can accomplish electrokinetic focusing of charged species in the presence of fluid flow that opposes their electromigration and has been employed extensively for analyte preconcentration, [10][11][12][13][14] water desalination, 7,15 removal of excess fluid from blood plasma, 16 separation of particles, 17 and paper and droplet-based microextraction 18,19 among other applications.…”

Section: Introductionmentioning

confidence: 99%

Electrokinetic Enrichment and Label-Free Electrochemical Detection of Nucleic Acids by Conduction of Ions Along the Surface of Bioconjugated Beads for Point-of-Care Testing

Bērziņa

Peramune

Claus

et al. 2022

Preprint

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In this paper, we report a method to integrate electrokinetic pre-enrichment of nucleic acids within a bed of probe-modified microbeads with their label-free electrochemical detection. In this detection scheme, hybridization of locally enriched target nucleic acids to the beads modulates the conduction of ions along the bead surfaces. This is a fundamental advancement in that this mechanism is similar to that observed in nanopore sensors, yet occurs in a bed of microbeads with microscale interstices. In application, this approach has several distinct advantages. First, electrokinetic enrichment requires only a simple DC power supply, and in combination with non-optical detection, makes this method amenable to point-of-care application. Second, the sensor is easy to fabricate, comprised of a packed bed of commercially-available microbeads, which can be readily modified with a wide range of probe types, thereby making this a versatile platform. Finally, the sensor is highly sensitive (picomolar) despite the modest 100-fold pre-enrichment we employ here by faradaic ion concentration polarization (fICP). Further gains are anticipated under conditions for fICP focusing that are known to yield higher enrichment factors (up to 100,000-fold enrichment). Here, we demonstrate detection of 3.7 pM single-stranded DNA complementary to the bead-bound oligoprobe, following a 30-min single step of enrichment and hybridization. Our results indicate that a shift in the slope of a current-voltage curve (CVC) occurs upon hybridization, and that this shift is proportional to the logarithm of the concentration of target DNA. Finally, we investigate the proposed mechanism of sensing by developing a numerical simulation that shows an increase in ion flux through the bed of insulating beads given changes in surface charge and zeta potential consistent with our experimental conditions.

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“…These properties make the paper-fluidic device attractive in remote settings and resource limited settings. μPADs have been studied for point-of-care diagnostics in remote settings, in which diverse methods have been developed for separation and preconcentration of samples [4][5][6][7]. Recently, separation and preconcentration of biomolecules has been demonstrated with paper-based ion concentration polarization (ICP) platform.…”

Section: Introductionmentioning

confidence: 99%

Spontaneous diffusiophoretic separation in paper-based microfluidic device

Lee

Kim

2020

Micro and Nano Syst Lett

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Microfluidic paper-based analytical devices (μPADs) for separating particles have been playing a key role for point-ofcare diagnostics in the area of remote settings. While splendid separation methods using μPADs have been explosively developed, they still require external devices inducing external field. In this work, the spontaneous separation method in μPADs was suggested by leveraging convective flow (the imbibition of paper and nanoporous medium) and diffusiophoresis by ion exchange medium. Especially, the paper's fast imbibition was utilized as driving particles at the first stage, which results in fast overall processing in contrast to the spontaneous separation method of microfluidic chip integrated with only ion exchange medium. Therefore, our novel spontaneous selective preconcentration method based on μPADs would have key potential to be used in portable point-of-care devices in remote settings.

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Paper-based ion concentration polarization device for selective preconcentration of muc1 and lamp-2 genes

Cited by 17 publications

References 17 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2016–2018)

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2016–2018)

Electrokinetic Enrichment and Label-Free Electrochemical Detection of Nucleic Acids by Conduction of Ions Along the Surface of Bioconjugated Beads for Point-of-Care Testing

Spontaneous diffusiophoretic separation in paper-based microfluidic device

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