Paper-Based Microfluidic Device with Upconversion Fluorescence Assay

He, Mengyuan; Liu, Zhihong

doi:10.1021/ac403693g

Cited by 86 publications

(64 citation statements)

References 32 publications

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“…For instance, based on different acceptor entities such as organic dyes, Au NPs, MnO 2 nanosheets, carbon NPs, graphene and graphene oxide (GO), Liu and coworkers [192][193][194][195][196][197][198][199][200] proposed a series of UC-FRET pairs for the detection of diverse biomolecules including glucose, thrombin, DNA, matrix metalloproteinase-2 (MMP-2), CEA, and Kanamycin, etc. For point-of-care testing, they further designed a straightforward paper-based microfluidic device (namely UC-μPAD) for UC-FRET assays based on a normal office printing sheet with a simple plotting method [192]. Similar paper-based UC-FRET platforms were also developed by Krull and coworkers [201,202] for DNA hybridization assays.…”

Section: Upconverting Luminescent Bioassaymentioning

confidence: 99%

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Huang

Zheng

et al. 2015

Sci. China Mater.

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Luminescent bioassay techniques have been widely adopted in a variety of research and medical institutions. However, conventional luminescent bioassays utilizing traditional bioprobes like organic dyes and quantum dots often suffer from the interference of background noise from scattered lights and autofluorescence from biological matrices. To eliminate this disadvantage, the use of inorganic lanthanide (Ln 3+ )-doped nanoparticles (NPs) is an excellent option in view of their superior optical properties, such as the long-lived downshifting luminescence, near-infrared triggered anti-Stokes upconverting luminescence and excitation-free persistent luminescence. In this review, we summarize the latest advances in the development of inorganic Ln 3+ -doped NPs as sensitive luminescent bioprobes from their fundamental physicochemical properties to biodetection, including the chemical synthesis, surface functionalization, optical properties and their promising applications for background-free luminescent bioassays. Future efforts and prospects towards this rapidly growing field are also proposed. INTRODUCTIONSensitive and specific bioassay of trace amount of target analytes is essential for a variety of biomedical applications ranging from pharmaceutical preparation to disease diagnosis and therapy [1][2][3]. Among various bioassay methods, luminescent bioassay has received particular attention because of its high sensitivity and good specificity [4,5]. Conventional luminescent bioassay techniques such as enzyme-linked immunosorbent assay (ELISA), time-resolved (TR) fluoroimmunoassay (TRFIA), Förster resonance energy transfer (FRET) and TR-FRET assays have laid the foundation for many modern clinical applications [6][7][8][9][10][11]. However, these bioassays are impeded by the availability of traditional bioprobes like organic dyes, lanthanide (Ln 3+ ) chelates and quantum dots (QDs). The use of these bioprobes has a number of limitations. Organic dyes commonly possess poor photochemical stability and suffer from serious photobleaching [12]. The applicability of QDs is compromised by photoblinking and high toxic- ity of heavy metal elements like cadmium and selenium, especially for in vivo biosensing [13,14]. Moreover, both organic dyes and QDs may induce high background noise and considerable photodamage to the biological samples under ultraviolet (UV) excitation, which deteriorates their detection sensitivity for bioassays [15,16]. Although such background noise can be suppressed by the technique of TR photoluminescence (PL) through the use of the longlived luminescence of Ln 3+ -chelates, the poor photochemical stability, long-term toxicity and high cost of Ln 3+ -chelates remain a major issue [17]. These concerns fuel a crucial demand for the development of a new generation of luminescent bioprobes to circumvent the limitations of traditional ones.Recently, with the explosion of nanoscience and nanotechnology, there is a growing dedication towards the development of diverse luminescent nanoprobes for biodetection ...

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Section: Upconverting Luminescent Bioassaymentioning

confidence: 99%

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Huang

Zheng

et al. 2015

Sci. China Mater.

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“…The main driver for the huge research and development activities in academia and industry has been its foreseen potential to produce scientific breakthroughs and practical devices in life sciences, medical, pharmaceutical, and chemical industries, environmental monitoring, and biosecurity applications. [1][2][3][4][5][6][7][8][9] Over the years, extensive varieties of theoretical investigations and experimental studies have been realized to prove the various advantages of microfluidic devices over conventional analytical procedures. The core of microfluidics is its intrinsic ability to manipulate very small volumes of fluids in a wide variety of integrated ways including fast sample processing, accurate control of fluids and delivery of results with a fast turnaround time.…”

Section: Introductionmentioning

confidence: 99%

“…In the case of typical down-conversion fluorescent detection, strict requirements are set for the paper quality due to the strong autofluorescence of paper additives significantly limiting the number of suitable paper materials. 9 Therefore, other detection methods, such as colorimetry, have been used in paper-based microfluidics. [19][20][21] To achieve the benefits of fluorescence detection, with the most notably low limit of detection, fluorescent upconversion sensing has been developed for paperbased microfluidics.…”

Section: Introductionmentioning

confidence: 99%

“…[19][20][21] To achieve the benefits of fluorescence detection, with the most notably low limit of detection, fluorescent upconversion sensing has been developed for paperbased microfluidics. 9 For medical applications, paper is an attractive material as it is inexpensive and provides an integrated flammable material for chip disposal. The protocol for the disposal of analytical accessories is generally strictly regulated when dealing with potentially harmful substances, such as blood samples.…”

Section: Introductionmentioning

confidence: 99%

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Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

et al. 2018

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bcde Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, userfriendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS-paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.

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“…To reduce the cumbersome immunoassay steps and obtain quick information from solution samples, fluorescence resonance energy transfer (FRET) was introduced, in FRET process, the energy is transferred from the donor to the acceptor through non-radiative dipole-dipole interactions [21][22][23][24][25][26][27]. In fact, before an effective FRET occurs, two conditions must be met as: (i) the fluorescent donor molecule and an acceptor molecule that are brought in a close proximity to each other; (ii) a strong overlap between the emission spectrum of the donor and the absorption spectrum of the acceptor [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Upconversion fluorescence resonance energy transfer—a novel approach for sensitive detection of fluoroquinolones in water samples

Zhang

et al. 2016

Microchemical Journal

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A novel homogeneous assay method was put forth for simultaneous sensing of fluoroquinolone derivatives (FQs) in water utilizing the upconversion fluorescence resonance energy transfer (FRET) process based on the competitive reaction between dissolved FQs and labeled AuNPs for FQs Mab connected UCPs. The prepared size-tunable β-NaLuF4:Yb,Er,Gd upconversion phosphors (UCPs) were functionalized with carboxylic acid (COOH-) and monoclonal antibody (Mab, C2F3C2) to act as a donor. The gold nanoparticles (AuNPs) labeled with the corresponding antigen (ciprofloxacin-BSA) take the role of an acceptor. Under optimized conditions, the limit of detections (LODs) for three common FQs, enrofloxacin (ENR), ciprofloxaxin (CIP), and norfloxacin (NOR), were 0.19-0.32 ng/mL based on 3σ. The recoveries were found to be in the range of 73.5-114.5% (normalized value according to the cross-reactivity of Mab) for water samples (tap water, pond water and river water). The proposed approach possesses significant advantages as follows: (i) simple procedure (only one step) without washing and separation steps, and no sample pretreatment other than filtration; (ii) fast liquid-phase kinetics with shortened incubation time; and (iii) high tolerance to various interfering substances. All of which indicated its potentiality as an efficient biosensor towards the monitoring of FQs in aquatic environments.

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Paper-Based Microfluidic Device with Upconversion Fluorescence Assay

Cited by 86 publications

References 32 publications

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

Upconversion fluorescence resonance energy transfer—a novel approach for sensitive detection of fluoroquinolones in water samples

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