Paper-based microfluidic sampling and separation of analytes for potentiometric ion sensing

Ding, Jiawang; He, Ning; Lisak, Grzegorz; Qin, Wei; Bobacka, Johan

doi:10.1016/j.snb.2016.11.128

Cited by 38 publications

(32 citation statements)

References 45 publications

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“… 64 To achieve better results, commercially available paper substrates can be modified by different nanomaterials 65 or chemically impregnated. 66 …”

Section: Discussionmentioning

confidence: 99%

Ion Transfer Voltammetry with an Electrochemical Pen

et al. 2020

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We present a new electrochemical system that combines paper-based sensing and ion-transfer voltammetry, bringing the latter a step closer toward point-of-care applications. Studies at the interface between two immiscible electrolyte solutions (ITIES) are often performed to detect redox-inactive species; unfortunately, due to the inherent instability of the interface, it is rather poorly explored outside specialized laboratories. Here, we address this limitation by combining a pen-like device containing the gelled organic phase with a paper-supported aqueous phase. This combination makes the system more user-friendly, potentially low-cost, and easy to assemble. We show the applicability of the new cell to analyze both simple and ionophore-facilitated transfer of ions and proteins, preconcentration of species, and analysis of mixtures through combination with paper chromatography. The native ion content of the paper also enabled measurements without added electrolytes. Those studies could broaden the scope for the application of the label-free electrochemical detection of nonredox-active species at points-of-need.

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“… 64 To achieve better results, commercially available paper substrates can be modified by different nanomaterials 65 or chemically impregnated. 66 …”

Section: Discussionmentioning

confidence: 99%

Ion Transfer Voltammetry with an Electrochemical Pen

et al. 2020

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“…To explore this mechanism, it is necessary to promote a controlled passive flow of a marker ion through the sensing membrane. In conventional ISE operated in zero current potentiometry, the inner reference solution is the source of this ion flow towards the sample solution, but this electrode design poses several practical limitations (Cui et al, 2014;Ding et al, 2017;Lisak et al, 2015). Lisak, G et al (Lisak et al, 2015) studied the possibility of using filter paper sampling coupled to potentiometric detection with all-solid-state and the sampling filter paper matrix may influence the response of the sensors towards some positive ions.…”

Section: Working Sensing Mechanism: Proofing the Theorymentioning

confidence: 99%

Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogen

Silva

Almeida

Magalhães

et al. 2019

Biosensors and Bioelectronics

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This work reports a new paper-based sensing platform and its application in a label-free potentiometric immunosensor for Salmonella typhimurium detection based on the blocking surface principle. A paper-based strip electrode was integrated with a filter paper pad which acted as a reservoir of the internal solution. The design offers a convenient platform for antibody immobilization and sampling, proving also that is a simple and affordable methodology to control an ionic flux through a polymer membrane. Two different immunosensing interfaces were assembled on the developed paper-strip electrode. The simplest interface relied on direct conjugation of the antibody to the polymer membrane and the second one resorted to an intermediate layer of a polyamidoamine dendrimer, with an ethylenediamine core from the fourth generation. Electrochemical impedance spectroscopy was used to assess the successive interface modification steps and the resulting analytical performance of both immunosensors was compared. For such, the potential shift derived from the blocking effect of the ionic flux caused by antigen-antibody conjugation was correlated with the logarithm of the Salmonella typhimurium concentration in the sample. In optimized conditions, a limit of detection of 5 cells mL −1 was achieved. As a proof-of-concept, the proposed method was applied to apple juice samples, demonstrating to be a suitable prototype to be used in real scenarios in useful time (< 1 h assay).

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“…aptamer, paper-based sensor, potentiometry, whole virus, zika Lisak, Qin, & Bobacka, 2017;Jin et al, 2018;Kawahara, Sahatiya, Badhulika, & Uno, 2018;Kofler, Nau, & List-Kratochvil, 2015;Lisak, Cui, & Bobacka, 2015;Nery & Kubota, Apr., 2016;Parrilla, Cánovas, & Andrade, 2017;Ping et al, 2013;Sjöberg et al, 2016) utilize gradients of ion distributions that generate open-circuit voltages (V oc ) that are measured to transduce analytes. Paperbased pathogen and virus sensors are also easy to incinerate.…”

Section: Introductionmentioning

confidence: 99%

Whole virus detection using aptamers and paper‐based sensor potentiometry

Dolai

Tabib‐Azar

2020

Med Devices & Sens

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Paper‐based sensors, microfluidic platforms, and electronics have attracted attention in the past couple of decades because they are flexible, can be recycled easily, environmentally friendly, and inexpensive. Here we report a paper‐based potentiometric sensor to detect the whole Zika virus with a minimum sensitivity of 0.26 nV/Zika and a minimum detectable signal (MDS) of 2.4x10 7 Zika. Our paper sensor works very similar to a P‐N junction where a junction is formed between two different regions with different electrochemical potentials on the paper. These two regions with slightly different ionic contents, ionic species and concentrations, produce a potential difference given by the Nernst equation. Our paper sensor consists of 2‐3 mm x 10 mm segments of paper with conducting silver paint contact patches on two ends. The paper is dipped in a buffer solution containing aptamers designed to bind to the capsid proteins on Zika. We then added the Zika (in its own buffer) to the region close to one of the silver‐paint contacts. The Zika virus (40 nm diameter with 43 kDa or 7.1x10 ‐20 gm weight) became immobilized in the paper’s pores and bonded with the resident aptamers creating a concentration gradient. Atomic force microscopy and Raman spectroscopy were carried out to verify that both the aptamer and Zika become immobilized in the paper. The potential measured between the two silver paint contacts reproducibly became more negative upon adding the Zika. We also showed that a Liquid Crystalline Display (LCD) powered by the sensor can be used to read the sensor output.

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Paper-based microfluidic sampling and separation of analytes for potentiometric ion sensing

Cited by 38 publications

References 45 publications

Ion Transfer Voltammetry with an Electrochemical Pen

Ion Transfer Voltammetry with an Electrochemical Pen

Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogen

Whole virus detection using aptamers and paper‐based sensor potentiometry

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