Paper Chromatographic Separation of the Coproporphyrin Isomers I and III

Sub-Uroporphyrins and their Occurrence

“…Decarboxylation and isomer TLC on cellulose plates with Lamon and With's [1973] modification of Eriksen's [1958] method, showed that the UP prepared consisted of about 90% of UP III and about 10% UP I. This was consistent with the macroscopic appearance of the crystals which, like turaco UP III ester, on evapora tion of the methanol from the crystallization mixture were transformed into dark granules quite different from the continuous light brown flakes of crystalline UP I ester.…”

The porphyrias: a review.

“…The individual porphyrins are then estimated either spectrophotometrically or fluorimetrically after elution from the plates or by fluorescence scanning in situ (Doss, Ulshofer, and Philipp-Domiston, 1971c). The sensitivity of these methods which have been used, particularly for urine, is increased if the porphyrins are converted to their zinc chelates before separation (Doss, 1971 (Eriksen, 1958), or in the case of uroporphyrins by decarboxylation to coproporphyrins which may then be characterized in the same way (Edmundson and Schwartz, 1953). Future Considerations It will be seen that the porphyrias are an interesting group of metabolic diseases.…”

“…This may be carried out in the case of coproporphyrins by chromatography in a lutidine:water:ammonia system (Eriksen, 1958), or in the case of uroporphyrins by decarboxylation to coproporphyrins which may then be characterized in the same way (Edmundson and Schwartz, 1953). Future Considerations It will be seen that the porphyrias are an interesting group of metabolic diseases.…”

Isolation and Characterization of a Vinylic Tricarboxyl Porphyrin from Bile

“…Porphyrin methyl esters be fore and after acetylation [6] procedure were subjected to paper chromatography using the method of Chu et al [7]. The separation of porphyrin-free acids was achieved on paper using the method of Eriksen [8]. Where necessary, porphyrinfree acids were separated, prior to spectrophotometric analysis, using a TLC method described previously The mass spectra of porphyrin methyl esters were obtained using an AEI MS9 instrument with a direct inlet system.…”