Sub-Uroporphyrins and their Occurrence

Rimington, C.; With, Torben K.

doi:10.1159/000459302

Cited by 9 publications

(7 citation statements)

References 9 publications

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“…The use of the solvent mixture described above, however, kept the uroporphyrin position clear of the haematin spot in most of the chromatograms. Haematoporphyrin, a hydroxyporphyrin, might be formed from protoporphyrin by addition of water to vinyl groups in the presence of sulphuric acid, and this would be visible on our chromatograms as a spot high in the space between the origin and the uroporphyrin region (Rimington & With, 1974). Faint spots of this sort are to be seen in Fig.…”

Section: Discussionmentioning

confidence: 77%

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Porphyrins in Erythrocytes and Blood Plasma: Preliminary Studies with Qualitative Thin-Layer Chromatography

With

1977

Clinical Science

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1. A simple chromatographic method for the study of porphyrins in erythrocytes and blood plasma, requiring little technician time, is described. The findings in adults and neonates are presented as well as those from severely ill patients. 2. The regular occurrence of uro-, copro- and proto-porphyrin in erythrocytes is deomonstrable as well as sporadic occurrence of tri-, penta-, hexa- and hepta-carboxylic porphyrin. The method gives, however, irregular values for protoporphyrin, which seems to occur in a more labile form in some subjects than in others; this is especially the case in erythropoietic protoporphyria. 3. In blood plasma, uroporphyrin occurs regularly, and coproporphyrin less regularly. Protoporphyrin and penta- to hepta-carboxylic porphyrin are found only occasionally.

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Section: Discussionmentioning

confidence: 77%

“…With this technique, a certain amount of partially esterified matter results, which is located below uroporphyrin on the chromatograms and can be removed by re-esterscation of the evaporated chloroform ester extract (With, 1973;Rimington & With, 1974). Thus it is not necessary to dry the erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

Porphyrins in Erythrocytes and Blood Plasma: Preliminary Studies with Qualitative Thin-Layer Chromatography

With

1977

Clinical Science

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“…), a modification of the method of Doss (1969) (cf. Rimington & With, 1974). In this system the partial esters remain in the sub-uro region, clearly separated from the per-esters.…”

Section: Methodsmentioning

confidence: 99%

“…light in darkness of the chromatogram of the solution after the CU2+ titration will reveal any fluorescence left on this position. The silica-gel plates were prepared from suspensions of silica gel H (Merck, Darmstadt, Germany) in methanol and were ready for use in 1 h without further activation (With, 1973;Rimington & With, 1974). The method worked only with such plates and not with prefabricated SiO2/foil plates.…”

Section: Tlc Indicator Methodsmentioning

confidence: 99%

Molar absorption coefficients of porphyrin esters in chloroform determined by copper titration

With¹,

Pedersen²

1977

Biochemical Journal

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Chromatographically pure porphyrin esters free from metalloporphyrins were titrated with Cu2+ to determine the molar amount of porphyrin present. The end point of the titration was defined by a t.l.c. method detecting traces of metal-free porphyrin ester in admxture to its copper complex. From spectrometric measurement at the Soret maximum and the molar amounts found by the titration, epsilonM was calculated for proto-, copro-, penta-, hexa- and hepta-carboxylic and uro-porphyrin permethyl esters. The values for uroporphyrin showed perfect agreement with those of previous workers, whereas those for coproporphyrin were about 5% lower and those for protoporphyrin more than 20% lower. The implications of the findings are discussed. Determinations of epsilonM of some higher esters (ethyl to pentyl) and some partial methyl esters with one carboxyl group free are also presented.

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“…The porphyric bull's blood showed slightly lower synthesis from porphobilinogen than did blood from the normal bulls and addition of reduced glutathione did not result in significant changes. The porphyrin pattern resulting from incubation systems from normal bulls, with both aminolaevulinic acid and porphobilinogen, was 40% uroporphyrin, 25% coproporphyrin, 15% heptacarboxylic porphyrin and 20% so-called suburoporphyrins (Rimington & With, 1974), i.e. porphyrins placed below the uroporphyrin position in t.1.c.…”

Section: Biosynthetic Activitymentioning

confidence: 99%

Studies on Porphyrin Biosynthesis and the Enzymes Involved in Bovine Congenital Erythropoietic Porphyria

et al. 1979

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1. Porphyrin biosynthesis and the enzymes succinyl-CoA synthetase (EC 6.2.1 S), Gaminolaevulinate synthase (EC 2.3.1.37), Gaminolaevulinate dehydratase (EC 4.2.1.24) and porphobilinogenase enzyme complex at 37°C and 45OC were studied in blood, bone marrow, spleen, liver and kidney of normal bulls and a bull with congenital erythropoietic porphyria (CEP). rinogen in the urine. The blood plasma contained only traces of porphyrin. Enzyme determinations showed threefoldincreased succinyl-CoA synthase in CEP bone marrow as compared with normal animals, manyfold increase of aminolaevulinate synthase, normal aminolaevulinate dehydratase, sevenfold increase of porphobilinogenase activity on incubation at 45OC and threefold increase at 37OC. In blood, aminolaevulinate synthase and aminolaevulinate dehydratase were increased in CEP, whereas porphobilinogenase at 45OC was reduced. In liver, aminolaevulinate synthase was normal and porphobilinogenase decreased. 6.Our results show that in this animal with CEP there was an increased porphobilinogen deaminase and a significant enhancement of aminolaevulinate synthase concomitant with a diminished activity of uroporphyrinogen I11 cosynthase.

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Sub-Uroporphyrins and their Occurrence

Cited by 9 publications

References 9 publications

Porphyrins in Erythrocytes and Blood Plasma: Preliminary Studies with Qualitative Thin-Layer Chromatography

Porphyrins in Erythrocytes and Blood Plasma: Preliminary Studies with Qualitative Thin-Layer Chromatography

Molar absorption coefficients of porphyrin esters in chloroform determined by copper titration

Studies on Porphyrin Biosynthesis and the Enzymes Involved in Bovine Congenital Erythropoietic Porphyria

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