Paper Chromatography of Alkaloids Using Liquid Ion Exchangers as Developing Solvents

Soczewińaski, Edward; Matysik, G.; Szumilo, Halina

doi:10.1080/01496396708049915

Cited by 8 publications

(1 citation statement)

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“…These results imply that the relative rates of chromatographic movement of a group of glucosiduronic acids in a system of chloroform-tetraheptylammonium chloride-water should be different from the relative rates of movement of the same compounds in carbon tetrachloride-tetraheptylammonium chloride-water. It is well known (Kunin & Winger, 1962;Soczewinski et al, 1967) that the diluent used as a solvent for ion-exchangers influences both the solvent power and the selectivity of the solution for separating groups of compounds.…”

Section: Discussionmentioning

confidence: 99%

Extraction of steroidal glucosiduronic acids from aqueous solutions by anionic liquid ion-exchangers

Mattox

Litwiller

Goodrich

1972

Biochemical Journal

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A pilot study on the extraction of three steroidal glucosiduronic acids from water into organic solutions of liquid ion-exchangers is reported. A single extraction of a 0.5mm aqueous solution of either 11-deoxycorticosterone 21-glucosiduronic acid or cortisone 21-glucosiduronic acid with 0.1m-tetraheptylammonium chloride in chloroform took more than 99% of the conjugate into the organic phase; under the same conditions, the very polar conjugate, beta-cortol 3-glucosiduronic acid, was extracted to the extent of 43%. The presence of a small amount of chloride, acetate, or sulphate ion in the aqueous phase inhibited extraction, but making the aqueous phase 4.0m with ammonium sulphate promoted extraction strongly. An increase in the concentration of ion-exchanger in the organic phase also promoted extraction. The amount of cortisone 21-glucosiduronic acid extracted by tetraheptylammonium chloride over the pH range of 3.9 to 10.7 was essentially constant. Chloroform solutions of a tertiary, a secondary, or a primary amine hydrochloride also will extract cortisone 21-glucosiduronic acid from water. The various liquid ion exchangers will extract steroidal glucosiduronic acid methyl esters from water into chloroform, although less completely than the corresponding free acids. The extraction of the glucosiduronic acids from water by tetraheptylammonium chloride occurs by an ion-exchange process; extraction of the esters does not involve ion exchange.

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Section: Discussionmentioning

confidence: 99%