Paper electrophoresis as a quantitative method. Serum proteins

Jencks, William P.; Jetton, Mera R.; Durrum, E. L.

doi:10.1042/bj0600205

Cited by 142 publications

(36 citation statements)

References 19 publications

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“…This method depends on the fact that the absorbance at 220 mu of a dipeptide in solution is greater than the absorbance of a solution of equivalent amounts of its constituent amino acids (Fig. 2) 11,000 g in an angle head centrifuge which was allowed to stop without braking. Approximately 1 ml of each supernatant solution was transferred to a dry, acid-washed quartz cuvette (1.0 cm light path), and the absorbance at 220 mls (Zeiss PMQ II spectrophotometer, slit width 0.34 mm) of an assay mixture was read against its corresponding blank mixture.…”

Section: Introductionmentioning

confidence: 99%

Peptide hydrolase activities of the mucosa of human small intestine

Heizer¹,

Laster²

1969

J. Clin. Invest.

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A B S T R A C T Few studies have been published on peptide hydrolase activities of human small intestine mucosa. We developed methods to screen tissue extracts for such enzymes and to quantitate hydrolase activities for dipeptides containing the aromatic amino acid L-phenylalanine. The screening procedure indicated glyCyl-L-proline hydrolase activity was reduced in biopsy specimens from patients with flattened intestinal mucosa. To explore this further, we established optimal assay conditions for hydrolaseBiopsy specimens from patients with various intestinal disorders, but without flattened mucosa, and from three patients with flattened mucosa, showed a disproportionate reduction in activities (a) and (b), with the reduction being significantly more marked in the latter patients. We suggest that intestinal imidopeptide hydrolase activities, such as (a) and (b), are sensitive to changes in intestinal disease generally, particularly to the altered physiology associated with flattening of the mucosa, and are secondary to, rather than a cause of, the intestinal pathology.Our finding that intestinal alkaline phosphatase activity tended to parallel imidopeptide hydrolase activity, and that activity (a) was partially localized to the particulate fraction of mucosal homogenate, suggested that imidopeptide hydrolase activities may be located in the microvilli of the intestinal epithelium and that, like alkaline phosphatase activity, they may be reduced in flattened mucosae, in part at least because of the pathologic changes in the microvilli.Dr. Heizer's present address is

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Section: Introductionmentioning

confidence: 99%

Peptide hydrolase activities of the mucosa of human small intestine

Heizer¹,

Laster²

1969

J. Clin. Invest.

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“…Sera were analyzed by electrophoretic separation on filter paper in veronal buffer at pH 8.6 for 16 hours using a Spinco Model M Apparatus. Details of the method as well as the procedure used in dying the paper strips are available elsewhere (12). The dye stained strips were scanned with a Spinco Model RA Analytrol Densitometer and the per cent composition of the electrophoretic components calculated from the tracings of the automatic integrator.…”

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confidence: 99%

Serum Protein Changes in Thermal Trauma. I. Electrophoretic Analysis at pH 8.6

Lanchantin¹,

Deadrick²

1958

J. Clin. Invest.

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“…Total proteins were determined by the biuret method (9), and the quantity of albumin was determined both by paper electrophoresis (10) and by Howe sodium sulfate fractionation. Dialysis of labeled albumin preparations and selected specimens of urine, bile, and gastric juice was performed for 3 hr against 25%o polyvinylpyrrolidone at 40C with two changes of the dialysis fluid.…”

Section: Chemical Methodsmentioning

confidence: 99%

“…Lugol solution, five drops three times daily, was given for 2 days before and throughout the duration of the study. The amount of labeled albumin necessary to provide approximately 10 ,uc of '2I and 60-80 ,uc of "Cr was weighed into a plastic syringe and was injected intravenously. The syringe was rinsed three times with venous blood before withdrawal of the needle.…”

Section: Patient Studiesmentioning

confidence: 99%

Use of 125I- and 51Cr-Labeled Albumin for the Measurement of Gastrointestinal and Total Albumin Catabolism*

Kerr¹,

Bois²,

Holt³

1967

J. Clin. Invest.

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Abstract. A method for the simultaneous measurement of gastrointestinal protein loss and total albumin turnover entailing the use of a combination of 125iodine-and 51chromium-labeled albumin is described. Albumin turnover was calculated by the measurement of albumin-125I plasma decay and cumulative urinary excretion, and the results obtained agreed closely with previous studies utilizing albumin-'31I. Gastrointestinal catabolism was calculated from the rate of fecal excretion of 51Cr and the specific activity of plasma albumin-51Cr, and these data were related to the calculated albumin turnover results. During the period of 6-14 days after administration, the ratio of specific activties of albumin-125I and -51Cr in plasma and in extravascular spaces or gastric and biliary secretions remained almost identical. Fecal excretion of 51Cr was also quite stable at this time. In six normal subjects gastrointestinal catabolism accounted for less than 10% of total albumin catabolism. Excessive gastrointestinal protein losses did not contribute to the low serum albumin in three patients with cirrhosis or in two adults with the nephrotic syndrome. Multiple mechanisms leading to hypoalbuminemia were demonstrated in other subjects with a variety of gastrointestinal disorders.

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Paper electrophoresis as a quantitative method. Serum proteins

Cited by 142 publications

References 19 publications

Peptide hydrolase activities of the mucosa of human small intestine

Peptide hydrolase activities of the mucosa of human small intestine

Serum Protein Changes in Thermal Trauma. I. Electrophoretic Analysis at pH 8.6

Use of 125I- and 51Cr-Labeled Albumin for the Measurement of Gastrointestinal and Total Albumin Catabolism*

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