Paper Electrophoretic Study of Human Fetal Serum Proteins with Demonstration of a New Protein Fraction

Bergstrand, C. G.; Czar, B.

doi:10.3109/00365515709079971

Cited by 108 publications

(33 citation statements)

References 10 publications

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“…The serum of the human fetus contains a protein that is not present in the sera of normal children or adults (1,2). This protein has been observed in the serum of the conceptus as early as 10 to 14 weeks in the gestational period, and it has been reported that the serum concentration of the protein decreases as gestation continues (1)(2)(3)(4)(5)(6)(7).…”

mentioning

confidence: 99%

“…This protein has been observed in the serum of the conceptus as early as 10 to 14 weeks in the gestational period, and it has been reported that the serum concentration of the protein decreases as gestation continues (1)(2)(3)(4)(5)(6)(7). Most investigators have been unable to detect the protein in fetuses older than 4 to 6 months' gestation (1)(2)(3)(4)(5)(6)8) or in prematurely born infants (5), but others have reported its presence in cord serum and in the serum of the newborn during the first week of life (9,10).…”

mentioning

confidence: 99%

“…The electrophoretic mobility of this serum fetoprotein at pH 8.6 is that of an a-globulin, the protein migrating somewhat slower than serum albumin but faster than the serum a1-globulins at this pH (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). As is well known, fetal calf serum also contains an a-globulin, the mucoprotein fetuin (11), which is not found in adult bovine serum.…”

mentioning

confidence: 99%

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Serum alpha-fetoprotein, albumin, and gamma-G-globulin in the human conceptus.

Gitlin¹,

Boesman²

1966

J. Clin. Invest.

407

101

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Serum alpha-fetoprotein, albumin, and gamma-G-globulin in the human conceptus.

Gitlin¹,

Boesman²

1966

J. Clin. Invest.

407

101

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“…Alpha-fetoprotein (AFP) is a glycoprotein of Mr 70 000 first discovered in human fetal blood by Bergstrand & Czar (1957). It was later found in the blood of hepatoma-bearing mice by Abelev et ai (1963) and in tumour-bearing human patients by Tatarinov (1964).…”

Section: Introductionmentioning

confidence: 99%

Alpha-fetoprotein can regulate growth in the uterus of the immature and adult ovariectomized mouse

Mizejewski¹,

Warner²

1989

Reproduction

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Summary. This is a report of development of (1) a 3-day immature mouse bioassay for alpha-fetoprotein (AFP) to increase the working range in uterine wet weights overcoming seasonality, and (2) a bioassay for AFP performed with ovariectomized adult mice to increase sensitivity. Mouse AFP was isolated from amniotic fluid and purified by polyacrylamide gel electrophoresis followed by Blue Sepharose chromatography. The uterine growth evoked by the injection of 1\m=.\0 \ g=m\ g AFP together with excess molar oestradiol (0\m=.\5\g=m\g) over a 23-h period was compared in immature ovariectomized adult Nylar mice. The 3-day assay with immature mice was rendered usable in any season, with sensitivity comparable to the 1-day assay. Increased sensitivity, however, was demonstrated by utilization of AFP in a 1-day assay with the adult ovariectomized mouse.

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“…Subsequently, the same protein fraction was identified in a number of mammalian species (Gitlin & Boesman, 1967a). Human AFP was thought to be a fetus-specific serum protein (Bergstrand & Czar, 1957), but high levels of this protein in adult human serum are also associated with primary liver cancer and other hepatic diseases (Kew, 1974). AFP is synthesized in the fetal liver and yolk sac of the fetal rat and is secreted into the fetal circulation (Gitlin & Boesman, 1967b), ultimately appearing in the maternal circulation.…”

Section: Introductionmentioning

confidence: 99%

Rat alpha-fetoprotein in experimental utero-placental ischaemia

Lai¹,

Hay²,

Forrester³

et al. 1976

Reproduction

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Summary. Retardation of growth and death of fetal rats were produced after utero\x=req-\ placental ischaemia was induced by surgical ligation of the uterine arteries. Changes in maternal plasma levels of alpha-fetoprotein (AFP) were measured by radioimmunoassay. In rats in which the uterine blood supply was totally occluded, the resultant increase in maternal plasma AFP was due to resorption of fetal elements, because AFP levels in maternal rat plasma did not increase following hysterectomy in a control group. Maternal plasma AFP levels in rats with a partly occluded blood supply (and therefore some dead and some live fetuses) paralleled those of sham-operated rats, suggesting that increased placental transfer of AFP to maternal plasma may have offset the anticipated decline of AFP due to a decreased number of live fetuses.

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Paper Electrophoretic Study of Human Fetal Serum Proteins with Demonstration of a New Protein Fraction

Cited by 108 publications

References 10 publications

Serum alpha-fetoprotein, albumin, and gamma-G-globulin in the human conceptus.

Serum alpha-fetoprotein, albumin, and gamma-G-globulin in the human conceptus.

Alpha-fetoprotein can regulate growth in the uterus of the immature and adult ovariectomized mouse

Rat alpha-fetoprotein in experimental utero-placental ischaemia

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