Paper/polymer composited microfluidic platform for screening cell viability and protein expression under a chemical gradient environment

Lei, Kin Fong; Goh, Andrew; Huang, Chun‐Hao

doi:10.1016/j.talanta.2019.120124

Cited by 7 publications

(4 citation statements)

References 43 publications

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“…The cells left in the channels also result in an uneven number of cells in each culture chamber. Recently, the paper/polymer composited and paper-based microfluidic chip for concentration gradient production and cell analysis have been reported; , these solved the problems of pipe blockage and uneven distribution of cells in the culture chamber and could provide a 3D culture environment and chemical gradient to cells, but the manufacture of these microfluidic chips involves many steps. Herein, we introduced a novel platform integrated 3D-printed microfluidic chip and paper-based chip.…”

Section: Resultsmentioning

confidence: 99%

Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell’s Apoptosis and Intracellular Cross-Talk

Liu

et al. 2020

ACS Sens.

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In this paper, we first proposed a novel hybrid three-dimensional (3D) printed and paper-based microfluidic platform and applied it for investigating the cell’s apoptosis and intracellular cross-talk. The fabrication of a 3D-printed microfluidic chip is much easier than polydimethylsiloxane (PDMS) chip and can be applied in many common labs without soft lithogrophy fabrication equipment. Moreover, 3D printing can be perfectly combined with paper-based chips that can provide 3D scaffold for cell culture and analysis. In addition, these paper chips are disposable after use, greatly reducing the experimental cost. We integrated “Christmas Tree” structure with the top layer of the 3D-printed microfluidic chip to generate a continuous concentration gradient, and the bottom layer contained paper-based chips as cell culture area. The two-layer structure allows the concentration gradient forming layer to be separated from the cell culture layer, which can simplify the planting of cells in the microfluidic chip and make sure the cells stay in the culture chambers and don’t clog the microfluidic channels. Applying this hybrid platform, we examined the effect of H2S on cancer cells. Continuous exposure to a low concentration of H2S inhibited cancer cell SMMC-7721 proliferation by inducing cell apoptosis. We also found that two gaseous molecules H2S and NO have cross-talk in cancer cells; they formed bioactive intermediate polysulfides in cancer cells. It is expected that this novel hybrid 3D-printed and paper-based microfluidic platform will have widespread application prospects in cell investigation.

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Section: Resultsmentioning

confidence: 99%

Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell’s Apoptosis and Intracellular Cross-Talk

Liu

et al. 2020

ACS Sens.

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“…The chosen paper must possess sufficient mechanical strength to resist deformation in liquid environments, exhibit appropriate hydrophilicity or hydrophobicity to form test regions and demonstrate chemical stability without reacting with samples. Currently, filter paper serves as the preferred substrate material due to its excellent water absorption, resistance to deformation, affordability, and availability [12][13][14][15]. Additionally, filter paper features a smooth, flat surface with uniform properties, ideal fluid flow rates, and high particle retention.…”

Section: Fabrication Strategy Of Paper-based Microfluidic Chipsmentioning

confidence: 99%

Paper-Based Microfluidic Chips for Food Hazard Factor Detection: Fabrication, Modification, and Application

Liang,

Zhang,

Song

et al. 2023

Foods

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Food safety and quality are paramount concerns for ensuring the preservation of human life and well-being. As the field of food processing continues to advance, there is a growing interest in the development of fast, instant, cost-effective, and convenient methods for detecting food safety issues. In this context, the utilization of paper-based microfluidic chips has emerged as a promising platform for enabling rapid detection, owing to their compact size, high throughput capabilities, affordability, and low resource consumption, among other advantages. To shed light on this topic, this review article focuses on the functionalization of paper-based microfluidic surfaces and provides an overview of the latest research and applications to colorimetric analysis, fluorescence analysis, surface-enhanced Raman spectroscopy, as well as their integration with paper-based microfluidic platforms for achieving swift and reliable food safety detection. Lastly, the article deliberates on the challenges these analytical methods and presents insights into their future development prospects in facilitating rapid food safety assessment.

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“…In recent years, the microfluidic platform has become a prevalent platform for experimental cell research. [107][108][109][110][111] F I G U R E 4 (A) The structure concept diagram of the microfluidic graphene field-effect transistor (GFET) biosensor. Reproduced from Reference [24] with permission of the Royal Society of Chemistry.…”

Section: Cellmentioning

confidence: 99%

“…In recent years, the microfluidic platform has become a prevalent platform for experimental cell research. [ 107–111 ] Microfluidic technology can complete the test in a relatively short time with a small amount of samples. The applications of microfluidic technology in cell biology range from single cells to flow cytometry analogs, which can be analyzed.…”

Section: Microfluidic Applicationsmentioning

confidence: 99%

Polymer‐based microfluidic devices: A comprehensive review on preparation and applications

Han

Zhang

Tian

et al. 2021

Polymer Engineering & Sci

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As key components in life science, microfluidics devices play increasingly important roles in the fields of biomedicine, pharmaceuticals, and biology due to its high detection efficiency. Also, microfluidics devices have many applications in label-free detection, thanks to their low cost and ease of operation.Traditionally, microfluidic devices are fabricated with glass and silicon materials. As an alternative, polymer materials are commonly used in the fabrication of microfluidic devices for their lower cost, versatile fabrication methods, biocompatibility, and repeatability. In this review, the methods for fabricating polymer microfluidic systems are described in detail, including lithography, hot embossing and imprinting, soft lithography, laser ablation, 3D printing, and other technologies. In particular, the merits and demerits of each of the methods are discussed comprehensively. This review also focuses on the applications of polymer-based microfluidic systems in the areas of biology, medicine and production, typical application scenarios including blood, tumor cell and nucleic acid testing, oil adsorption detection, and pesticide detection. This review emphasizes the device preparation methods and application scenarios of polymer-based microfluidic systems and was expected to arise more extensive discussions in this field.

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Paper/polymer composited microfluidic platform for screening cell viability and protein expression under a chemical gradient environment

Cited by 7 publications

References 43 publications

Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell’s Apoptosis and Intracellular Cross-Talk

Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell’s Apoptosis and Intracellular Cross-Talk

Paper-Based Microfluidic Chips for Food Hazard Factor Detection: Fabrication, Modification, and Application

Polymer‐based microfluidic devices: A comprehensive review on preparation and applications

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