Paper Strip Whole Cell Biosensors: A Portable Test for the Semiquantitative Detection of Bacterial Quorum Signaling Molecules

Struss, Anjali K.; Pasini, Patrizia; Ensor, Mark; Raut, Nilesh; Daunert, Sylvia

doi:10.1021/ac100231a

Cited by 100 publications

(56 citation statements)

References 44 publications

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“…Most of the previously developed whole-cell biosensors deliver an optical output [39]. In a previous work, Struss et al developed a whole-cell biosensor detecting AHLs of gram-negative bacteria, particularly P. aeruginosa [40]. Similar to the approach presented herein, they used components of the AHL-mediated QS regulatory system to generate an optical signal.…”

Section: Discussion and Outlookmentioning

confidence: 99%

Development of a Modular Biosensor System for Rapid Pathogen Detection

Hanke¹,

Bailly²,

Demling³

et al. 2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Progress in the field of pathogen detection relies on at least one of the following three qualities: selectivity, speed, and cost-effectiveness. Here, we demonstrate a proof of concept for an optical biosensing system for the detection of the opportunistic human pathogen Pseudomonas aeruginosa while addressing the abovementioned traits through a modular design. The biosensor detects pathogen-specific quorum sensing molecules and generates a fluorescence signal via an intracellular amplifier. Using a tailored measurement device built from low-cost components, the image analysis software detected the presence of P. aeruginosa in 42 min of incubation. Due to its modular design, individual components can be optimized or modified to specifically detect a variety of different pathogens. This biosensor system represents a successful integration of synthetic biology with software and hardware engineering.

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Section: Discussion and Outlookmentioning

confidence: 99%

Development of a Modular Biosensor System for Rapid Pathogen Detection

Hanke¹,

Bailly²,

Demling³

et al. 2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

View full text Add to dashboard Cite

show abstract

“…These sensors are suitable for in vitro and in situ measurements [42]. As described earlier, reporter methods include luminescent, fluorescent and colorimetric signals ( Table 2), which required widely available equipment for laboratory usage as well as for in situ measurements [43].…”

Section: Choosing the Adequate Quorum Sensing Biosensormentioning

confidence: 99%

Detection and Control of Indoor Airborne Pathogenic Bacteria by Biosensors Based on Quorum Sensing Chemical Language: Bio-Tools, Connectivity Apps and Intelligent Buildings

Ibacache-Quiroga¹,

Romo²,

Díaz-Viciedo³

et al. 2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Nowadays, lifestyles and climate change lead people to spend long periods in indoors spaces, where reduced ventilation and artificial light favor the concentration and spread of airborne pathogenic microorganisms. Current procedures for microbiological air evaluation are based on air sampling coupled to traditional microbiological culturedependent methods such as biochemical tests and molecular rDNA 16S sequencing. These techniques generate an important delay in the application of prevention and control measures. This chapter presents whole cell-based biosensors that are able to detect quorum sensing signaling molecules produced by airborne pathogenic bacteria as a tool for indoor air monitoring. Furthermore, a general biosensor model is proposed. In this model, in vivo biosensors technology can be connected to online applications (Apps), being part of intelligent buildings, in order to reduce airborne pathogenic bacteria concentration and dissemination.

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“…Bacterial quorum signalling (QS) molecules, N-acyl homoserine lactones (AHLs), have been used as a sensing mechanism [140]. The bacterial cell-based sensing system comprised two main components: (i) AHL-mediated QS regulatory system as recognition elements, and (ii) β-galactosidase as the reporter enzyme.…”

Section: Genetically-engineered Cellsmentioning

confidence: 99%

Point-of-Care Diagnostics

Yetisen

2014

Holographic Sensors

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Rapid tests that are low-cost and portable are the first line of defence in healthcare systems. Dipstick and lateral-flow are the two universal assay formats as they are lightweight and compact, and provide qualitative results without external instrumentation. However, existing formats have limitations in the quantification of analyte concentrations. Hence, the demand for sample preparation, improved sensitivity and user-interface has challenged the commercial products. Recently, capabilities, sensors and readout devices were expanded to multiplexable assays platforms, which might transcend the capabilities of existing design format of diagnostic tests. This chapter outlines the evolution of diagnostic devices and current trends in the development of qualitative and quantitative sensing devices for applications in healthcare, veterinary medicine, environmental monitoring and food safety. The chapter also discusses design parameters for diagnostics, their functionalisation to increase the capabilities and the performance, emerging sensing platforms and readout technologies. The factors which limit the emerging rapid diagnostics to become commercial products are also discussed.The life expectancy has grown worldwide, which also increased the healthcare spending [1, 2]. For example, 720 million people will be aged 65 or older by 2020. Currently four in five people over the age of 75 take at least one prescribed medicine, and this trend is set to increase [3]. For some of the medications, large pharma find it the hard to recover R&D costs while the pressure from the regulatory agencies also increased for the use of new drugs as the first line of defence based on efficacy and cost. Hence, the global healthcare trends and ever increasing pressure from regulatory agencies put a strong case for the development of diagnostics for healthcare monitoring as well as the evaluation of drug efficacies in clinical trials. All these considerations parallels governments' and insurance companies' interests in obtaining the best performance possible and treatment benefits they support. These trends are behind the driving force for the development of diagnostics that can reduce the healthcare costs by developing effective drugs and identifying diseases and conditions at an early stage.The major stumbling block in monitoring and controlling diseases/contaminations remains delivering simple, low-cost and robust diagnostic tests [4,5]. In the developing world, the basic healthcare infrastructure and trained healthcare personnel are

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Paper Strip Whole Cell Biosensors: A Portable Test for the Semiquantitative Detection of Bacterial Quorum Signaling Molecules

Cited by 100 publications

References 44 publications

Development of a Modular Biosensor System for Rapid Pathogen Detection

Development of a Modular Biosensor System for Rapid Pathogen Detection

Detection and Control of Indoor Airborne Pathogenic Bacteria by Biosensors Based on Quorum Sensing Chemical Language: Bio-Tools, Connectivity Apps and Intelligent Buildings

Point-of-Care Diagnostics

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