Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

Rypar, Tomas; Adam, Vojtěch; Vaculovičová, Markéta; Macka, Mirek

doi:10.1016/j.snb.2021.129999

Cited by 25 publications

(5 citation statements)

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“…Because their general processes rely on the length of color changes in the detection area, corresponding to the analyte level, the analyte solution can flow along the detection channel and then react with a deposited specific reagent that was predeposited. − The results show the changes in the color length on the dPADs, illustrating the need for an equipment-free detection system for onsite analysis. With these properties, it can be noticed that there were numerous developments of dPADs for various detections with different reactions including enzymatic, metal nanoparticle aggregation, and complexation reactions. − Therefore, dPAD sensors are more advantageous for point of care (POC) testing in resource-poor settings because they do not require external equipment to evaluate the data for quantification. − …”

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confidence: 99%

A Simple Counting-Based Measurement for Paper Analytical Devices and Their Application

et al. 2022

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This work introduces the concept of a counting-based measurement on paper analytical devices (cPADs) to improve the utilization of numerous reactions. The design of cPADs consists of two layers of paper substrates; the first layer contains a central sample zone combined with a radial surrounded by 12 detection zones that are predeposited with the various reagents, and the second layer acts as a connection channel between the sample zone and each detection zone. The solution can vertically flow from the first to the second layer and then move through the area to each subsequent detection zone. The analyte level can be evaluated by counting the number of detection zones that change color from a blank signal. Furthermore, our cPADs exhibit a capability of implementation for a broad series of reactions. Compared to the dPAD technique, some reactions that are possibly difficult to apply in such devices can be wholly enabled in our devices. The final color reaction on cPADs can apparently occur due to its identity. We applied this technique to the monitoring of carbaryl (CBR) and copper ions (Cu2+) using different reactions, including azo-coupling and complexation, respectively. Accordingly, this indicates an excellent result validated using the more traditional methods. Our cPADs can be applied for rapid screening of both CBR and Cu2+ in water samples with outstanding accuracy and precision using a naked-eye measurement by a relatively unskilled person. We offer a simple platform on PADs for rapid screening, combining high cost-effectiveness within a miniaturized platform designed for use with onsite applications, which is thus suitable for several different reactions.

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confidence: 99%

A Simple Counting-Based Measurement for Paper Analytical Devices and Their Application

et al. 2022

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“…As these excited electrons revert to their ground state, they emit blue fluorescence. When the target analytes selectively bind to the MIP layer through hydrogen bonding interactions and van der Waals forces, 29,33 it leads to PET between the target analytes and the CDs, resulting in the quenching of their fluorescence emission. 27,28,30,41 Similarly, the quenching system in this technique is categorized as dynamic quenching.…”

Section: Working Principle and Feasibility Testsmentioning

confidence: 99%

“…[25][26][27][28] The principle behind MIP technology involves the use of molecular templates to generate selective binding sites within crosslinked polymers, leading to the creation of specific recognition binding sites for target analytes. [29][30][31][32] Interestingly, Rypar et al 33 successfully developed a dPADs integrated with MIP technique for chymotrypsinogen determination in human urine samples. Their approach used dopamine as a monomer for surface imprinting onto the detection channel due to its simple self-polymerization to form polydopamine.…”

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confidence: 99%

Distance-based paper analytical device for multiplexed quantification of cytokine biomarkers using carbon dots integrated with molecularly imprinted polymer

Khachornsakkul,

Del-Rio-Ruiz,

Chheang

et al. 2024

Lab Chip

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“… 27 Several MIPs, including MIPs targeting proteins, have been imprinted on paper. 28 These are used mainly used in sensing systems, e.g., MIPs for chymotrypsinogen determination in urine using a distance detection‐based microfluidic paper 29 and a fluorescent MIP for ferritin determination. 30 However, MIPs for the capture of small molecules (metabolites) from urine have been polymerized on paper for analysis by paper spray MS. 31 Hence, the production of MIPs on paper for protein analysis by MS should also be feasible.…”

Section: Reshuffling the Workflow In Mass Spectrometric Protein Analy...mentioning

confidence: 99%

Is this the end of dried blood spots as we know it?

Halvorsen

Reubsaet

2023

Analytical Science Advances

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In 2017 integrated sampling and sample preparation for simplified liquid chromatography‐mass spectrometry analysis of proteins from dried blood spots were introduced. The concept, called smart samplers or smart sampling, enables proteolysis or affinity clean‐up, two common sample preparation steps in liquid chromatography‐mass spectrometric bioanalysis of proteins, to start at the moment of sampling. The idea is to utilize the time for sampling and drying to perform these time‐consuming and labour‐intensive steps. Hence, only a simplified sample preparation is necessary after the arrival of the sample in the lab. In this perspective, we present an overview of the smart sampling approach where the conventional protein analysis workflow is reshuffled to start already prior to arrival in the lab. In addition, we present a thorough discussion of integrating sample preparation steps such as proteolysis or affinity capture in the sampling. Finally, in the end, we try to answer the question if conventional dried blood spots will become obsolete in the future.

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Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

Cited by 25 publications

References 50 publications

A Simple Counting-Based Measurement for Paper Analytical Devices and Their Application

A Simple Counting-Based Measurement for Paper Analytical Devices and Their Application

Distance-based paper analytical device for multiplexed quantification of cytokine biomarkers using carbon dots integrated with molecularly imprinted polymer

Is this the end of dried blood spots as we know it?

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