Paracoccus sediminilitoris sp. nov., isolated from a tidal flat sediment

Abstract

A novel marine Gram-stain-negative, non-spore-forming, motile, aerobic, coccoid or ovoid bacterium, designated as strain DSL-16 T , was isolated from a tidal flat sediment on the East China Sea and characterized phylogenetically and phenotypically. Optimal growth of the strain occurred at 35 C (range 4-40 C), at pH 6 (range 5-11) and with 4 % (w/v) NaCl (range 1-14 %). The nearest phylogenetic neighbour was Paracoccus seriniphilus DSM 14827 T (98.2 % 16S rRNA gene sequence similarity). The digital DNA-DNA hybridization value between strain DSL-16 T and P. seriniphilus DSM 14827 T was 19.5±2.2 %. The average nucleotide identity value between strain DSL-16 T and P. seriniphilus DSM 14827 T was 83.6 %. The sole respiratory ubiquinone was Q-10. The major polar lipids were phosphatidylmonomethylethanolamine (PME), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), diphosphatidyglycerol (DPG) and glycolipid (GL). The predominant cellular fatty acids of strain DSL-16 T were C 18 : 1 !7c, C 18 : 0 and 11-methyl C 18 : 1 !7c. The G+C content of the genomic DNA was 64.5 mol%. The combined genotypic and phenotypic data indicated that strain DSL-16 T represents a novel species of the genus Paracoccus, for which the name Paracoccus sediminilitoris sp. nov. is proposed. The type strain is DSL-16 T (=KCTC 62644 T =MCCC 1K03534 T).

Muricauda hadalis sp. nov., a novel piezophile isolated from hadopelagic water of the Mariana Trench and reclassification of Muricauda antarctica as a later heterotypic synonym of Muricauda teanensis

“…and the second dimension was chloroform/glacial acetic acid/methanol/water (80 : 15 : 12 : 4, by vol.). Other reagents such as α-naphthol, ninhydrin and molybdenum blue (Sigma) were used to detect glycolipids, aminolipids and phospholipids, according to Tindall [28]. The plates were then sprayed with sulfuric acid/ethanol (1 : 1, v/v) and heated at 120 °C for 10 min to reveal total lipids according to previously established procedures [29].…”

“…indica 3PC125-7 T [9], with PE as the major lipid, which were also present in almost all species of the genus Muricauda . Respiratory quinones were extracted using the method described by Minnikin et al [27] and analysed by HPLC as described by Tindall [28]. The sole respiratory quinone was MK-6, which was the same as other species of the genus Muricauda .…”

Paracoccus xiamenensis sp. nov., isolated from seawater on the Xiamen

“…At the time of writing, the genus Paracoccus contained 68 recognized species with validly published names (www.bacterio.net/paracoccus.html) [2]. Species of genus Paracoccus were isolated from various environments, including water [3–5], air [6], soil [7, 8], rhizosphere [9, 10], activated sludge [11, 12], sediment [13–15] and fish [16]. This study describes the taxonomic position of strain 12-3 T by a polyphasic approach.…”

Paracoccus amoyensis sp. nov., isolated from the surface seawater along the coast of Xiamen Island, China

“…The purity of strain 11-3 T was confirmed by uniformity of cell morphology and 16S rRNA gene sequence. In this study, four type strains of Paracoccus caeni MJ17 T [17], Paracoccus xiamenensis 12-3 T [18], Paracoccus pacificus F14 T [10] and Paracoccus denitrificans DSM 413 T [19] were used as reference strains for phenotypic and fatty acid analysis. During the study, strain 11-3 T and reference strains were cultured on MA plates or in marine broth 2216 (MB, BD Difco) at 28 °C unless where indicated.…”

“…xiamenensis 12-3 T (66.9 mol%) and P. pacificus F14 T (61.4 mol%), with their respective differences of 1.4, 6.2, 6.8 and 1.3 % [1–10, 10–18, 37]. Based on differences <1.0 % (cut-off value for defining a new species), strain 11-3 T can be considered as a new species [38].…”