Paracrine Effect of Synovial-Derived Stem Cells on Induced Knee Osteoarthritis in Rats

Abstract

Background: Osteoarthritis (OA) is a progressive degenerative disease. Previous treatment strategies have tried to slow the progress of OA. Objectives: The present study aimed to stop and reverse the progressive nature of OA by the paracrine effects of synovial stem cells. Methods: Fifty male Sprague Dawley rats were randomly allocated to five equal groups (n = 10). The control group received no treatment. The second group received an intra-articular Hyalgan injection. The third group received an intra-articular injection of secreta. The fourth group received an intra-articular injection of synovial-derived stem cells (5 × 106). The last group received an intra-articular injection of secreta combined with synovial-derived stem cells (5 × 106). Three months after treatment, the samples were harvested and evaluated by histopathological and radiological analyses. Results: Histopathological and radiological findings demonstrated significant differences between the synovial stem cell combined with the secreta group and the control and Hyalgan groups. Significant differences were observed in the subchondral bone and matrix scores between the secreta group and the synovial stem cell and Hyalgan groups (P-value = 0.042 and P-value = 0.0001, respectively). Both secreta and synovial stem cell groups showed better healing in terms of cell population viability index than the Hyalgan group (P-value = 0.015 and P-value = 0.005, respectively). The synovial stem cell combined with secreta group showed a significant difference from the synovia stem cell group in both medial femoral condyle and fabella osteophyte indices (P-value = 0.004 and P-value = 0.011, respectively). Conclusions: The group treated with synovial stem cells combined with secreta showed better outcomes than other groups in histopathological and radiological evaluations.

Induced Knee Osteoarthritis: Preventive Effects of Wharton-jelly Mesenchymal Stem Cell Conditioned Medium, Captopril and Losartan in Male Rats

“…Tang et al (2017) evaluated the effects of subcutaneous AMSCs injection in OA rats and showed that the cartilage surface was smooth along with the good distribution of chondrocytes (42).In line with these results, our previous study also showed that treatment with synovial stem cells accompanied with secreta resulted in columnar-cluster arranged chondrocyte while in the only-stem cell treated group the surface was continuous composed of brocartilage tissue (43). In this study, we also observed that treatment with WJ-CM decreased the thickness and increased the cell distribution in articular surface compared OA, OA + HA, OA + Cap.…”

Attenuation of osteoarthritis progression through intra-articular injection of a combination of synovial membrane-derived MSCs (SMMSCs), platelet-rich plasma (PRP) and conditioned medium (secretome)

“…After 24 h, the conditioned medium was collected and centrifuged twice, first at 500× g for 10 min and afterward at 3000× g for 20 min, to remove cell debris. Finally, pre-cleaned secretome was concentrated to ~ 1.5 mL using Centriprep YM-3 centrifugal units (Millipore, Bedford, MA, USA) [ 30 ].…”

Novel strategy of cartilage repairing via application of P. atlantica with stem cells and collagen

“…The animals underwent anesthesia by intramuscular injection of 10 mg/kg xylazine HCl (Alfasan, Netherlands) and 90 mg/kg ketamine HCl (Alfasan, Netherlands). OA was induced based on a previously reported and approved method 37,38 . The knee of the rats was shaved and 500 IU collagenase type II (clostridium histolithicum) dissolved in sterile PBS (Phosphate Buffered Saline), was injected (days 0).…”

“…OA was induced based on a previously reported and approved method. 37,38 The knee of the rats was shaved and 500 IU collagenase type II (clostridium histolithicum) dissolved in sterile PBS (Phosphate Buffered Saline), was injected (days 0). The second injection of collagenase was repeated with the same dose on day 3.…”