Paradoxical DNA Repair and Peroxide Resistance Gene Conservation in Bacillus pumilus SAFR-032

Gioia, Jason; Yerrapragada, Shailaja; Qin, Xiang; Jiang, Huaiyang; Igboeli, Okezie; Muzny, Donna M.; Dugan-Rocha, Shannon; Ding, Yan; Hawes, Alicia; Li, Wen; Perez, Lesette; Kovar, Christie; Dinh, Huyen; Lee, Sandra; Nazareth, Lynne V.; Blyth, Peter R; Holder, Michael; Buhay, Christian; Tirumalai, Madhan R.; Liu, Yamei; Dasgupta, Indrani; Bokhetache, Lina; Fujita, Masaya; Karouia, Fathi; Moorthy, Prahathees Eswara; Siefert, Johnathan; Uzman, Akif; Buzumbo, Prince; Verma, Avani; Zwiya, Hiba; McWilliams, Brian D.; Olowu, Adeola; Clinkenbeard, Kenneth D.; Newcombe, David; Golebiewski, Lisa; Petrosino, Joseph F.; Nicholson, Wayne L.; Fox, George E.; Venkateswaran, Kasthuri; Highlander, Sarah K.; Weinstock, George M.

doi:10.1371/journal.pone.0000928

Cited by 117 publications

(117 citation statements)

References 71 publications

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“…Endospores of B. pumilus SAFR-032 are resistant to high concentrations of H 2 O 2 (5% for 60 min) and simulated Martian UV irradiation (29,38). The SAFR-032 genome was sequenced, and numerous protein-encoding genes were identified as putative candidates responsible for the strain's enhanced resistance to the various sterilization treatments tested (21). Understanding its hydrogen peroxide resistance is particularly important since H 2 O 2 is used in current and newly developed sterilization methods (29,46).…”

mentioning

confidence: 99%

Protection of Bacillus pumilus Spores by Catalases

Checinska

Burbank

Paszczyński

2012

Appl Environ Microbiol

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ABSTRACTBacillus pumilusSAFR-032, isolated at spacecraft assembly facilities of the National Aeronautics and Space Administration Jet Propulsion Laboratory, is difficult to kill by the sterilization method of choice, which uses liquid or vapor hydrogen peroxide. We identified two manganese catalases, YjqC and BPUM_1305, in spore protein extracts of severalB. pumilusstrains by using PAGE and mass spectrometric analyses. While the BPUM_1305 catalase was present in six of theB. pumilusstrains tested, YjqC was not detected in ATCC 7061 and BG-B79. Furthermore, both catalases were localized in the spore coat layer along with laccase and superoxide dismutase. Although the initial catalase activity in ATCC 7061 spores was higher, it was less stable over time than the SAFR-032 enzyme. We propose that synergistic activity of YjqC and BPUM_1305, along with other coat oxidoreductases, contributes to the enhanced resistance ofB. pumilusspores to hydrogen peroxide. We observed that the product of the catalase reaction, gaseous oxygen, forms expanding vesicles on the spore surface, affecting the mechanical integrity of the coat layer, resulting in aggregation of the spores. The accumulation of oxygen gas and aggregations may play a crucial role in limiting further exposure ofBacillispore surfaces to hydrogen peroxide or other toxic chemicals when water is present.

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confidence: 99%

Protection of Bacillus pumilus Spores by Catalases

Checinska

Burbank

Paszczyński

2012

Appl Environ Microbiol

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“…UVR-resistant microorganisms have shown tremendous stable biotechnological implications [2,3,29,30]. The short exposure of UV is generally known for random mutagenesis, however microbial reversion may produce unstable mutants with commercially viability.…”

Section: Occurrence Of Microorganisms At Higher Altitudes and Uvrmentioning

confidence: 99%

“…[32]. Gioia et al [29] have reported several genes involved in DNA repair and oxidative stress (dinG, disA, dnaE, mutL, ogt, pcrB, polA, polY2, recD, sms, uvrX, uvrA, uvrB, sodA, sodF, bcrC, and many more caused by UV-C radiation). Two novel species of Bacillus, B. nealsonii, isolated from a spacecraft assembly facility, and B. odysseyi, were resistant to UV radiation [39,40].…”

Section: Tolerance and Survivability Of Uvrresistant Extremophilesmentioning

confidence: 99%

Bio-signature of Ultraviolet-Radiation-Resistant Extremophiles from Elevated Land

Gabani¹,

Prakash²,

Singh³

2014

AJMR

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Microorganisms with the ability to survive high doses of radiation are known as radiation-resistant extremophiles. This study attempts to demonstrate the diversity of microorganisms resistant to ultraviolet radiation (UVR) in the natural environment in order to investigate the molecular and physiological mechanisms by which these microorganisms survive under extreme radiation. We hypothesized that topsoil from elevated land (hills) would reveal a diverse variety of UVR-resistant extremophiles with modulated proteins/enzymes.

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“…The bacterial species surviving highly unfavorable conditions have always been enigmatic to the scientifi c world and can provide a lot of interesting information regarding genes and factors which facilitate them to do so. Bacillus pumilus SAFR-032 is one such organism that produces spores highly resistant to unfavorable conditions like UV radiation, gamma-radiation, oxidative stress, desiccation, chemical disinfection, or starvation and have been sequenced by Baylor College of Medicine [47]. Both the vegetative cells and the spores of this strain are found to be more resistant to UV and H 2 O 2 as compared to other Bacillus species and it even survives the decontamination procedures of the Jet Propulsion Lab spacecraft assembly facility.…”

Section: Revealing Extreme Livingmentioning

confidence: 99%

“…Its 3.7 Mb genome 3687 ORFs, 12 frameshifts, 38 pseudogenes, 7 rRNA operons, 69 tRNAs, and 21 ncRNAs. The whole genome data also revealed many genes relevant to DNA repair and the oxidative stress response and those were then compared to the less resistant bacillus species B. subtilis and B. licheniformis [47]. The B. pumilus genome includes genes not found in B. subtilis or B. licheniformis and conserved genes with sequence variations, but surprisingly lacks several genes that function in UV or H 2 O 2 resistance in other Bacillus species.…”

Section: Revealing Extreme Livingmentioning

confidence: 99%

From bacterial genomics to metagenomics: concept, tools and recent advances

et al. 2008

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In the last 20 years, the applications of genomics tools have completely transformed the fi eld of microbial research. This has primarily happened due to revolution in sequencing technologies that have become available today. This review therefore, fi rst describes the discoveries, upgradation and automation of sequencing techniques in a chronological order, followed by a brief discussion on microbial genomics. Some of the recently sequenced bacterial genomes are described to explain how complete genome data is now being used to derive interesting fi ndings. Apart from the genomics of individual microbes, the study of unculturable microbiota from different environments is increasingly gaining importance. The second section is thus dedicated to the concept of metagenomics describing environmental DNA isolation, metagenomic library construction and screening methods to look for novel and potentially important genes, enzymes and biomolecules. It also deals with the pioneering studies in the area of metagenomics that are offering new insights into the previously unappreciated microbial world.

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Paradoxical DNA Repair and Peroxide Resistance Gene Conservation in Bacillus pumilus SAFR-032

Cited by 117 publications

References 71 publications

Protection of Bacillus pumilus Spores by Catalases

Protection of Bacillus pumilus Spores by Catalases

Bio-signature of Ultraviolet-Radiation-Resistant Extremophiles from Elevated Land

From bacterial genomics to metagenomics: concept, tools and recent advances

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