Paradoxical increase of sperm motility and seminal carnitine associated with moderate leukocytospermia in infertile patients

Ziyyat, Ahmed; Barraud-Lange, Virginie; Sifer, C.; Ducot, B.; Wolf, Jean-Philippe; Soufir, J. C.

doi:10.1016/j.fertnstert.2007.10.032

Cited by 32 publications

(32 citation statements)

References 56 publications

(58 reference statements)

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“…We also showed that even in the presence of leukocytospermia >10 6 /mL, and contrary to what was expected, leukocytes protect sperm function and are associated with an increased clinical pregnancy rate in ART compared with rates for infertility patients without leukocytospermia (1). We have also showed that moderate leukospermia appears to be physiologic and constitutes a favorable factor for sperm quality and functions (2), in contrast to high leukocytospermia, which is linked to prostatitis. In addition, elevated leukocytospermia does not hinder any sperm fertilization functions (1).…”

contrasting

confidence: 55%

Seminal leukocytes and clinical outcomes with donor sperm insemination

Barraud-Lange

Pont

Pocate

et al. 2011

Fertility and Sterility

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contrasting

confidence: 55%

Seminal leukocytes and clinical outcomes with donor sperm insemination

Barraud-Lange

Pont

Pocate

et al. 2011

Fertility and Sterility

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“…Therefore, in these patients there could be a condition of SV pseudo-inflammation of the seminal vesicles. Finally, we observed a paradoxical increase in sperm motility associated with moderate leucocytospermia in these patients but also other authors had reported this association in other clinical models (Ziyyat et al, 2008;Barraud-Lange et al, 2011).…”

Section: Discussionsupporting

confidence: 81%

Seminal vesicles and diabetic neuropathy: ultrasound evaluation after prolonged treatment with a selective phosphodiesterase‐5 inhibitor

et al. 2012

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SUMMARYWe have previously reported that infertile patients with diabetes mellitus (DM) have a particular ultrasound features of the seminal vesicles (SV) characterized by higher fundus-to-body ratio and lower pre-and post-ejaculatory difference in body antero-posterior diameter (APD). Based on these premises the aim of the present study was to investigate possible ultrasound SV changes in infertile patients with DM and diabetic neuropathy (DN), after prolonged administration of tadalafil (TAD) (a specific phosphodiesterase-5 inhibitor). To accomplish this, 20 infertile patients with symptomatic DN and erectile dysfunction were selected and arbitrarily divided into two groups which were assigned to: daily administration of 5 mg TAD for 3 months (Group A) (n = 10) and administration of placebo (Group B) (n = 10). All patients underwent to scrotal and prostate-vesicular transrectal ultrasound evaluation and semen analysis (Laboratory Manual for the Examination and Processing of Human Semen, WHO, 2010) before and after treatment. The following SV US parameters were recorded: (i) body APD; (ii) fundus APD; (iii) parietal thickness of the right and left SVs; and (iv) number of polycyclic areas within both SVs. We then calculated the following parameters: (i) fundus/body (F/B) ratio; (ii) difference of the parietal thickness between the right and the left SV and (iii) pre-and post-ejaculatory APD difference. In addition, we also evaluated the SV ejection fraction. Group A patients showed a significant reduction in F/B ratio and higher pre-and post-ejaculatory body SV APD difference compared with baseline or Group B after 3 months. These patients showed also a significant increase in SV ejection fraction and a significant improvement of the total sperm count, progressive motility, seminal levels of fructose, leucocytes and ejaculate volume. In conclusion, these results suggest that infertile DM patients with DN and erectile dysfunction had an improvement of ultrasound features suggestive of diabetic neuropathy after daily treatment with low doses of TAD.

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“…Leukocytospermia reduces sperm motility and produces a large amount of toxic ROS which reduces the sperm quality. Thus, it adversely affects the male fertility machinery [Lackner et al 2006;Lackner et al 2010;Ziyyat et al 2008]. Bacterial infection also leads to male infertility by affecting sperm quality and concentration.…”

Section: Leukocytospermia and Failure In Male Fertilitymentioning

confidence: 99%

“…Results of more recent studies suggest that leukocytes have negative impact on semen quality via ROS generation [Sharma et al 2001;Aziz et al 2004;Henkel et al 2005]. Ziyyat et al reported an increase in sperm motility in semen samples with moderate leukocytospermia (defined as seminal leukocytes <1 x 10 6 /ml), but observed a paradoxical decrease in sperm motility in semen samples exceeding a threshold of 1 x 10 6 leukocytes per milliliter [Ziyyat et al 2008].…”

Section: Introductionmentioning

confidence: 99%

In silicoanalysis of candidate proteins sharing homology withStreptococcus agalactiaeproteins and their role in male infertility

Parida

Samanta

2016

Systems Biology in Reproductive Medicine

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Leukocytospermia is a physiologic condition defined as human semen with a leukocyte count of >1 x 10 6 cells/ml that is often correlated with male infertility. Moreover, bacteriospermia has been associated with leukocytospermia ultimately leading to male infertility. We have found that semen samples with >1 x 10 6 /ml leukocytes and/or bacteriospermia have oxidative predominance as evidenced by augmented protein carbonyl and lipid peroxidation status of the semen which is implicated in sperm dysfunction. It has been reported that Streptococcus agalactiae is present in bacteriospermic samples. Previous research has shown that human leukocyte antigen beta chain paralog (HLA-DRB) alleles interact best with the infected sperm cells rather than the non-infected cells. Little is known about the interaction of major histocompatibility complex (MHC) present on leukocytes with the sperm upon bacterial infection and how it induces an immunological response which we have addressed by epitope mapping. Therefore, we examined MHC class II derived bacterial peptides which might have human sperm-related functional aspects. Twenty-two S. agalactiae proteins were obtained from PUBMED protein database for our study. Protein sequences with more than two accession numbers were aligned using CLUSTAL Omega to check their conservation pattern. Each protein sequence was then analyzed for T-cell epitope prediction against HLA-DRB alleles using the immune epitope database (IEDB) analysis tool. Out of a plethora of peptides obtained from this analysis, peptides corresponding to proteins of interest such as DNA binding response regulator, hyaluronate lyase and laminin binding protein were screened against the human proteome using Blastp. Interestingly, we have found bacterial peptides sharing homology with human peptides deciphering some of the important sperm functions. Antibodies raised against these probable bacterial antigens of fertility will not only help us understand the mechanism of leukocytospermia/ bacteriospermia induced male factor infertility but also open new avenues for immunocontraception. Abbreviations: AA: amino acid; ASA: antisperm antibodies; GBS: group B streptococcus; HLA: human leukocyte antigen; HAS3: hyaluronan synthase 3: IEDB: immune epitope database; MAPO2: O 6 -methylguanine-induced apoptosis 2; MHC: major histocompatibility complex; ROS: reactive oxygen species; Rosbin1: round spermatid basic protein 1; S. agalactiae: Streptococcus agalactiae; SA: sperm antigen; SPATA17: spermatogenesis associated protein17; SPNR: spermatid perinuclear RNA binding protein; TEX15: testis-expressed sequence 15 protein; TOPAZ: testis-and ovaryspecific PAZ domain-containing protein; TPABP: testis-specific poly-A binding protein; TPAP: testis-specific poly(A) polymerase; WHO: World Health Organization ARTICLE HISTORY

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Paradoxical increase of sperm motility and seminal carnitine associated with moderate leukocytospermia in infertile patients

Cited by 32 publications

References 56 publications

Seminal leukocytes and clinical outcomes with donor sperm insemination

Seminal leukocytes and clinical outcomes with donor sperm insemination

Seminal vesicles and diabetic neuropathy: ultrasound evaluation after prolonged treatment with a selective phosphodiesterase‐5 inhibitor

In silicoanalysis of candidate proteins sharing homology withStreptococcus agalactiaeproteins and their role in male infertility

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