Paradoxical role of tumor necrosis factor on metabolic dysfunction and adipose tissue expansion in mice

Martins, Laís Bhering; Oliveira, Marina C.; Menezes‐Garcia, Zélia; Rodrigues, Débora F.; Lana, Jaqueline Pereira; Vieira, Leda Quércia; Teixeira, Mauro Martins; Ferreira, Adaliene Versiani Matos

doi:10.1016/j.nut.2017.07.006

Cited by 18 publications

(6 citation statements)

References 48 publications

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“…The analysis was performed according to Martins et al [26], with some modifications. The pancreas was uniformly and systematically cross-sectioned into five parts, which were stained with hematoxylin and eosin (HE).…”

Section: Methodsmentioning

confidence: 99%

Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles Reduces Fasting Blood Glucose Levels without Compromising Insulinemia: A Preclinical Study

et al. 2019

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In vivo studies show the benefits of the trypsin inhibitor isolated from tamarind (Tamarindusindica L.) (TTI) seeds in satiety and obesity. In the present study, TTI nanoencapsulation (ECW) was performed to potentialize the effect of TTI and allow a controlled release in the stomach. The impact on glycemia, insulin, and lipid profile was evaluated in Wistar rats overfed with a high glycemic index diet (HGLI). Characterization of the nanoparticles and in vitro stability in simulated gastrointestinal conditions, monitored by antitrypsin activity and HPLC, was performed. ECW and empty nanoparticles (CW) were administered by gavage, using 12.5 and 10.0 mg/kg, respectively. Both nanoformulations presented a spherical shape and smooth surface, with an average diameter of 117.4 nm (24.1) for ECW and 123.9 nm (11.3) for CW. ECW maintained the antitrypsin activity (95.5%) in the gastric phase, while TTI was completely hydrolyzed. In Wistar rats, the nanoformulations significantly reduced glycemia and HOMA IR, and ECW increased HDL-c compared to CW (p < 0.05).Pancreas histopathology of animals treated with ECW suggested an onset of tissue repair. Thenanoencapsulation provided TTI protection, gradual release in the desired condition, and improvement of biochemical parameters related to carbohydrate metabolism disorders,without compromising insulinemia.

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Section: Methodsmentioning

confidence: 99%

Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles Reduces Fasting Blood Glucose Levels without Compromising Insulinemia: A Preclinical Study

et al. 2019

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“…The analysis was performed according to Martins et al [ 26 ] with some modifications. Sections of the tissues (3–4 μm) were stained with hematoxylin and eosin.…”

Section: Methodsmentioning

confidence: 99%

Adipocytes and intestinal epithelium dysfunctions linking obesity to inflammation induced by high glycemic index pellet-diet in Wistar rats

et al. 2018

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We investigated the inflammatory effect of a pellet-diet with high glycemic index and load (HGLI) on the histological organization of adipocytes, intestinal epithelium, and fat in liver and pancreas in adult male Wistar rats. Two groups (n=10) received for 17 weeks: (1) HGLI diet or (2) Standard diet (Labina®). Histological analyses of adipose tissue, jejunum, liver, and pancreas were performed. Stereology analysis, visceral adiposity index, gene expression, and immunohistochemistry of tumor necrosis factor-α (TNF-α) in visceral adipose tissue and plasma TNF-α were also assessed. The HGLI diet-induced hypertrophy of adipocytes with adipocyte volume density equal to 97.0%, cross-sectional area of adipocytes equivalent to 1387 µm² and a total volume of adipocytes of 6.97 cm³ an elevation of 8%, 25%, and 58%, respectively. Furthermore, the HGLI diet increased liver and pancreatic fat deposition, altered and inflamed the intestinal epithelia, and increased TNF-α gene expression (P=0.014) with a positive immunostaining in visceral adipose tissue and high plasma TNF-α in comparison with standard diet. The results suggest that this diet was able to generate changes commonly caused to solid diets with high fat or fructose-rich beverages. To the best of our knowledge, this is the first report in the literature concerning the properties of low-cost, sucrose-rich pellet-diet presenting high glycemic index and high glycemic load efficient on the development of obesity complications in Wistar rats that were subjected to diet-induced obesity. Therefore, the HGLI pellet-diet may be considered an effective tool to be used by the scientific community in experimental research.

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“…This is consistent with one previous study showing that TNFR1 deficiency does not impact mouse body weight (Toda et al 2010). Notably, there is also another study showing that TNFR1 deficiency decreased mouse body weight, but paradoxically increased adiposity (Martins et al 2017). As such, although further studies are still needed to address the inconsistency in the body weight and adiposity effects of TNF signaling deficiency, all of these studies have supported that the obesity-dependent mechanism may be trivial for the physiological role of TNF signaling in homeostatic regulation of glucose metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to the relatively consistent role of TNFα in body weight and glucose homeostasis, the role of TNFR1 in body weight and glucose homeostasis is largely controversial. Like TNFα −/− mice, TNFR1 −/− mice were ever shown to have decreased body weight (Martins, et al 2017). However, they had paradoxically increased adiposity and normal glucose tolerance (Martins et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Like TNFα −/− mice, TNFR1 −/− mice were ever shown to have decreased body weight (Martins, et al 2017). However, they had paradoxically increased adiposity and normal glucose tolerance (Martins et al 2017). Furthermore, there was another report showing that TNFR1 deficiency impacted neither mouse body weight nor glucose homeostasis (Toda, et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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TNF signaling impacts glucagon-like peptide-1 expression and secretion

Chen

Wei

Chen

et al. 2018

Journal of Molecular Endocrinology

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Numerous studies have implicated tumor necrosis factor α (TNFα) in the pathogenesis of type 2 diabetes. However, the role of its primary receptor, TNF receptor 1 (TNFR1), in homeostatic regulation of glucose metabolism is still controversial. In addition to TNFα, lymphotoxin α (LTα) binds to and activates TNFR1. Thus, TNFα and LTα together are known as TNF. To delineate the role of TNF signaling in glucose homeostasis, the present study ascertained how TNF signaling deficiency affects major regulatory components of glucose homeostasis. To this end, normal diet-fed male TNFR1 deficient mice (TNFR1-/-), TNFα/LTα/LTβ triple deficient mice (TNF/LT∆3), and their littermate controls were subjected to intraperitoneal glucose tolerance test, insulin tolerance test, and oral glucose tolerance test. The present results showed that TNFR1-/- and TNF/LT∆3 mice versus their controls had comparable body weight, tolerance to intraperitoneal glucose, and sensitivity to insulin. However, their tolerance to oral glucose was significantly increased. Additionally, glucose-induced insulin secretion assessments revealed that TNFR1 or TNF/LT deficiency significantly increased oral but not intraperitoneal glucose-induced insulin secretion. Consistently, qPCR and immunohistochemistry analyses showed that TNFR1-/- and TNF/LT∆3 mice versus their controls had significantly increased ileal expression of glucagon-like peptide-1 (GLP-1), one of the primary incretins. Their oral glucose-induced secretion of GLP-1 was also significantly increased. These data collectively suggest that physiological TNF signaling regulates glucose metabolism primarily through effects on GLP-1 expression and secretion and subsequently insulin secretion.

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Paradoxical role of tumor necrosis factor on metabolic dysfunction and adipose tissue expansion in mice

Cited by 18 publications

References 48 publications

Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles Reduces Fasting Blood Glucose Levels without Compromising Insulinemia: A Preclinical Study

Tamarind Trypsin Inhibitor in Chitosan–Whey Protein Nanoparticles Reduces Fasting Blood Glucose Levels without Compromising Insulinemia: A Preclinical Study

Adipocytes and intestinal epithelium dysfunctions linking obesity to inflammation induced by high glycemic index pellet-diet in Wistar rats

TNF signaling impacts glucagon-like peptide-1 expression and secretion

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