Paragonimus and paragonimiasis in Asia: An update

Yoshida, Ayako; Doanh, Pham Ngoc; Maruyama, Haruhiko

doi:10.1016/j.actatropica.2019.105074

Cited by 84 publications

(102 citation statements)

References 86 publications

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“…More than fifty Paragonimus species have been identified around the world in tropical and subtropical regions, mainly in Asia. Around one million people become infected every year according to WHO data [58] and close to twenty million people in Asia, the Americas and Africa could be exposed to or at risk of contracting the disease; however, its prevalence is not known exactly [59]. The infection's prevalence depends on the abundance of the infection in animals and the prevalence of infectious metacercariae in crustacean hosts [60].…”

Section: Foodborne Trematodiasesmentioning

confidence: 99%

“…Its life-cycle requires at least two intermediate hosts as well as the definitive one; its definitive hosts include several mammals, particularly felines, canines and humans, in which it lives in pairs within a fibrous cyst in the lungs, causing pleurisy and pneumonia/bronchitis [58]. The parasite's erratic migration pattern means that it could invade the brain or spinal cord, causing symptoms of meningitis and even host death.…”

Section: Foodborne Trematodiasesmentioning

confidence: 99%

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Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections

Avendaño

Patarroyo

2020

IJMS

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The World Health Organisation (WHO) has placed twenty diseases into a group known as neglected tropical diseases (NTDs), twelve of them being parasitic diseases: Chagas’ disease, cysticercosis/taeniasis, echinococcosis, food-borne trematodiasis, human African trypanosomiasis (sleeping sickness), leishmaniasis, lymphatic filariasis, onchocerciasis (river blindness), schistosomiasis, soil-transmitted helminthiasis (ascariasis, hookworm, trichuriasis), guinea-worm and scabies. Such diseases affect millions of people in developing countries where one of the main problems concerning the control of these diseases is diagnosis-based due to the most affected areas usually being far from laboratories having suitable infrastructure and/or being equipped with sophisticated equipment. Advances have been made during the last two decades regarding standardising and introducing techniques enabling diagnoses to be made in remote places, i.e., the loop-mediated isothermal amplification (LAMP) technique. This technique’s advantages include being able to perform it using simple equipment, diagnosis made directly in the field, low cost of each test and the technique’s high specificity. Using this technique could thus contribute toward neglected parasite infection (NPI) control and eradication programmes. This review describes the advances made to date regarding LAMP tests, as it has been found that even though several studies have been conducted concerning most NPI, information is scarce for others.

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Section: Foodborne Trematodiasesmentioning

confidence: 99%

Section: Foodborne Trematodiasesmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections

Avendaño

Patarroyo

2020

IJMS

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“…They are of socio-economic importance since the route of infection is mainly food borne (1). The life cycle of Paragonimus is relatively complex that requires a minimum of three hosts including a de nitive host and two intermediate hosts (2). The rst and second intermediate hosts are frequently snails belonging to the families Assimineidae and Hydrobiidae and crabs belonging to the families Potamidae and Parathelphusidae (3).…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular characterization of Paragonimus species isolated from freshwater crabs in Yunnan, China.

Shu¹,

Shude²,

Tian³

et al. 2020

Preprint

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Background Paragonimus species are highly prevalent in ethnic minority areas of Yunnan, China. The objective of the study is to isolate and identify Paragonimus from natural habitat and compare the phylogenetic diversity of Paragonimus in Yunnan province, China. Methods Metacercariae of Paragonimus was isolated from crabs and morphologic identification was performed by microscopy. Metacercariae were injected into experimental Paragonimus free Sprague Dawley rats. After 112 days, adult worms and eggs were isolated from multiple organs. Morphologic identification confirmed the initial identification. DNA was extracted from the adult worms and molecular characterization was performed by amplification and sequencing of CO1 and ITS2 regions followed by phylogenetic analysis. Results Out of 447 crabs captured, 186 crabs were found to be infected. A total of 5 occurrences of Paragonimus was observed from naturally infected crabs. Paragonimus microrchis (2), P,heterotremus (1), P.proliferus (1) and P.skrjabini (1) were isolated and identified. A total of 32 sequences downloaded from national center for biotechnology information and 5 sequences generated in the study was used for phylogenetic analysis. Phylogenetic analysis revealed robustness of the double loci method as against the single locus method with either CO1 or ITS2 alone. Considerable phylogenetic diversity depending on the geographic location was observed. Conclusion Paragonimus species isolated from Yunnan province, China were phylogenetically diverse and the analysis revealed the clustering of multiple species of Paragonimus isolated from different geographic locations.

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“…1 The symptoms of paragonimiasis include chronic cough, chest pain, dyspnea, and hemoptysis, which are similar to those of tuberculosis and lung cancer. 2 Paragonimiasis is diagnosed by confirming the presence of eggs in patients' sputum or fecal samples. 3 Typical computed tomography findings of paragonimiasis are focal pleural thickening and subpleural linear opacities, leading to the development of necrotic peripheral pulmonary nodules.…”

mentioning

confidence: 99%

“…4 Praziquantel is the treatment of choice. 2 The frequency of parasitic diseases, including paragonimiasis, may increase worldwide, considering global dietary habit changes and increasing international travel.…”

mentioning

confidence: 99%