We present a new gravity map between 45°-70° W and 5°-40° S integrating open source terrestrial gravity data of Argentina with the South American Gravity Model 2004 (SAGM04), a 5 min-arc resolution gravity model. The Bouguer anomaly map reveals a 2,000 km long linear gravity feature from 15° S to 30° S at longitude 55° W, with a steep horizontal gradient separating two gravity domains. The eastern domain is the Paraná basin, with NE-SW trending Bouguer anomalies of-80 mGal in average. The western domain comprises the Chaco-Paraná, Chaco-Tarija and Pantanal basins, with circular positive anomalies of up to 20 mGal in amplitude. Previous seismic studies mapped a thinner crust of less than 35 km in the western domain and the present gravity models indicate a 10 to 20 kg/m³ denser crust. On the other hand, the eastern domain has a thicker crust of more than 40 km. Seismic tomography models also show P-and S-wave velocity reduction in the western domain whereas high-velocity characterises the Paraná basin. These geophysical data indicate that the gravity gradient marks a transition between two distinct lithospheres. The gravity gradient is associated with a tectonic feature referred to as the Western Paraná suture/shear zone. Granites of 530 to 570 Ma ages, located parallel or over the gravity gradient, suggest a Neoproterozoic to Early Cambrian age suture/shear zone, thus approximately synchronous and parallel to the Pampean belt. Sediment corrected residual gravity map and its vertical derivative allow us to define the limits of the Rio Apa, Rio de la Plata and Rio Tebicuary cratons. Their eastern and western limits are the Western Paraná suture and the Pampean belt, respectively. This study unravels Precambrian tectonic elements concealed by the Phanerozoic sedimentary basins adding new constraints for the amalgamation history of SW Gondwana. Research Highlights Gravity map reveals the Neoproterozoic 2,000 km long Western Paraná suture/shear zone Geophysical delimitation of the Rio Apa, Rio Tebicuary and Rio de la Plata cratons Gravity anomalies of the Amazonian and Rio Apa cratons are distinct New tectonic features of SW Gondwana final amalgamation revealed Key Words SW Gondwana; intracontinental basins; cratons; sutures; gravity Research Highlights Gravity map reveals the Neoproterozoic 2,000 km long Western Paraná suture/shear zone Geophysical delimitation of the Rio Apa, Rio Tebicuary and Rio de la Plata cratons Gravity anomalies of the Amazonian and Rio Apa cratons are distinct New tectonic features of SW Gondwana final amalgamation revealed