Parallel detection experiment of fluorescence confocal microscopy using DMD

Wang, Qingqing; Jiao, Z.; Wang, Kangni; Gui, Kun; Guo, Hanming; Zhuang, Songlin

doi:10.1002/sca.21265

Cited by 11 publications

(3 citation statements)

References 19 publications

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“…Subcellular imaging with FAST M could be achieved using PMT arrays. Alternatively, fluorescence could be recorded from several regions of interest (ROIs), whereby each ROI is illuminated with light modulated at a distinct frequency using acousto-optic-modulated laser excitation ( Wu et al, 2006 ) or rapid switching of a digital micromirror device (DMD) ( Chang et al, 2016 ; Wang et al, 2016 ). This approach would allow recording fluorescence signals from different ROIs with one PMT.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous recording of multiple cellular signaling events by frequency- and spectrally-tuned multiplexing of fluorescent probes

Kierzek

Deal

Miller

et al. 2021

eLife

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Fluorescent probes that change their spectral properties upon binding to small biomolecules, ions, or changes in the membrane potential (Vm) are invaluable tools to study cellular signaling pathways. Here, we introduce a novel technique for simultaneous recording of multiple probes at millisecond time resolution: frequency- and spectrally-tuned multiplexing (FASTM). Different from present multiplexing approaches, FASTM uses phase-sensitive signal detection, which renders various combinations of common probes for Vm and ions accessible for multiplexing. Using kinetic stopped-flow fluorimetry, we show that FASTM allows simultaneous recording of rapid changes in Ca2+, pH, Na+, and Vm with high sensitivity and minimal crosstalk. FASTM is also suited for multiplexing using single-cell microscopy and genetically-encoded FRET biosensors. Moreover, FASTM is compatible with opto-chemical tools to study signaling using light. Finally, we show that the exceptional time resolution of FASTM also allows resolving rapid chemical reactions. Altogether, FASTM opens new opportunities for interrogating cellular signaling.

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Section: Discussionmentioning

confidence: 99%

Simultaneous recording of multiple cellular signaling events by frequency- and spectrally-tuned multiplexing of fluorescent probes

Kierzek

Deal

Miller

et al. 2021

eLife

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“…The system is composed of light source, DMD, optical system (Fourier lens, Fourier filter, reduction lens), software system, 3D mobile platform, etc. In the process of digital mask exposure, DMD has the advantages of large exposure area, high resolution, high production efficiency, and low cost [ 8 , 9 , 10 ], which is widely used in MEMS production [ 11 ], micro optical device processing [ 12 , 13 , 14 ], 3D micro-nano structure processing [ 15 , 16 , 17 ], pattern transfer of printed circuit board (PCB) [ 18 ], etc. The output beam from the light source is irradiated onto the DMD after passing through the reflector.…”

Section: Introductionmentioning

confidence: 99%

Path Planning of Pattern Transfer Based on Dual-Operator and a Dual-Population Ant Colony Algorithm for Digital Mask Projection Lithography

Wang

Han

et al. 2020

Entropy

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In the process of digital micromirror device (DMD) digital mask projection lithography, the lithography efficiency will be enhanced greatly by path planning of pattern transfer. This paper proposes a new dual operator and dual population ant colony (DODPACO) algorithm. Firstly, load operators and feedback operators are used to update the local and global pheromones in the white ant colony, and the feedback operator is used in the yellow ant colony. The concept of information entropy is used to regulate the number of yellow and white ant colonies adaptively. Secondly, take eight groups of large-scale data in TSPLIB as examples to compare with two classical ACO and six improved ACO algorithms; the results show that the DODPACO algorithm is superior in solving large-scale events in terms of solution quality and convergence speed. Thirdly, take PCB production as an example to verify the time saved after path planning; the DODPACO algorithm is used for path planning, which saves 34.3% of time compared with no path planning, and is about 1% shorter than the suboptimal algorithm. The DODPACO algorithm is applicable to the path planning of pattern transfer in DMD digital mask projection lithography and other digital mask lithography.

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“…The imaging speed and the observation time are limited by the concentration and photobleaching of the fluorescent agents. Finally, implementing fluorescence approaches with confocal detection or light-sheet microscopy , enables mapping of the molecular director field in three dimensions, however, raster or depth scanning approaches further limit the imaging speed to a few frames per second. ,, Although recent approaches have achieved millisecond temporal resolution, , these advances in imaging speed come at a cost of more complicated instrumentation with decreased applicability in studies of birefringent structure dynamics.…”

mentioning

confidence: 99%

Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics

Zhang

et al. 2021

ACS Photonics

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Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a multitude of polarization light microscopy techniques are often used to access steadystate properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performances of PSIM are validated by imaging a rotating wave plate and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to 350×350 μm 2 and a diffraction-limit spatial resolution of ∼2 μm. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions.

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Parallel detection experiment of fluorescence confocal microscopy using DMD

Cited by 11 publications

References 19 publications

Simultaneous recording of multiple cellular signaling events by frequency- and spectrally-tuned multiplexing of fluorescent probes

Simultaneous recording of multiple cellular signaling events by frequency- and spectrally-tuned multiplexing of fluorescent probes

Path Planning of Pattern Transfer Based on Dual-Operator and a Dual-Population Ant Colony Algorithm for Digital Mask Projection Lithography

Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics

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