Parallel metatranscriptome analyses of host and symbiont gene expression in the gut of the termite Reticulitermes flavipes

Tartar, Aurélien; Wheeler, Marsha M.; Zhou, Xuguo; Coy, Monique R.; Boucias, Drion G.; Scharf, Michael E.

doi:10.1186/1754-6834-2-25

Cited by 205 publications

(270 citation statements)

References 96 publications

(151 reference statements)

Supporting

Mentioning

250

Contrasting

Unclassified

Order By: Relevance

“…61) This appears to parallel a recent report that lignin degradation was observed in the gut of the termite Z. angusticollis, 62) but it has been found that homologous laccase genes in the genomes of various insects are involved in tanning cuticles. 63) Since the sequencing efforts in these metatranscriptomic analyses were too weak to be useful to predict the entire functions of the protistan microbiota, more exhaustive analysis is needed.…”

Section: Metagenomics and Metatranscriptomics Of Termite Gut Micsupporting

confidence: 72%

Diversity and Genomes of Uncultured Microbial Symbionts in the Termite Gut

Hongoh

2010

Bioscience, Biotechnology, and Biochemistry

157

129

View full text Add to dashboard Cite

Section: Metagenomics and Metatranscriptomics Of Termite Gut Micsupporting

confidence: 72%

Diversity and Genomes of Uncultured Microbial Symbionts in the Termite Gut

Hongoh

2010

Bioscience, Biotechnology, and Biochemistry

157

129

View full text Add to dashboard Cite

“…Until recently, metatranscriptomics had been limited to the microarray/high-density array technology (46,86,140) or analysis of mRNA-derived cDNA clone libraries (90,119). These approaches have produced significant insights into the gene expression of microbial communities but have limitations.…”

Section: Metatranscriptomicsmentioning

confidence: 99%

Metagenomic Analyses: Past and Future Trends

Simon

Daniel

2011

Appl Environ Microbiol

631

349

View full text Add to dashboard Cite

Metagenomics has revolutionized microbiology by paving the way for a cultivation-independent assessment and exploitation of microbial communities present in complex ecosystems. Metagenomics comprising construction and screening of metagenomic DNA libraries has proven to be a powerful tool to isolate new enzymes and drugs of industrial importance. So far, the majority of the metagenomically exploited habitats comprised temperate environments, such as soil and marine environments. Recently, metagenomes of extreme environments have also been used as sources of novel biocatalysts. The employment of next-generation sequencing techniques for metagenomics resulted in the generation of large sequence data sets derived from various environments, such as soil, the human body, and ocean water. Analyses of these data sets opened a window into the enormous taxonomic and functional diversity of environmental microbial communities. To assess the functional dynamics of microbial communities, metatranscriptomics and metaproteomics have been developed. The combination of DNA-based, mRNA-based, and protein-based analyses of microbial communities present in different environments is a way to elucidate the compositions, functions, and interactions of microbial communities and to link these to environmental processes.

show abstract

“…In the mammalian gut, the diversity of bacteria increases according to host diet, from carnivores to omnivores to herbivores (38). The intestinal bacteria of insects such as termites and aphids produce compounds that are essential and otherwise inaccessible for the host (e.g., detritus, phloem, sap, wood, and xylem) (28,63,64). Most studies on the relationships between insect diet and the distribution of gut microbiota have tended to focus on specific microbes in a single insect group; however, a recent, wider-ranging study based on previously published and newly generated 16S rRNA gene sequences from 62 insect species from seven orders reported that both host diet and taxonomy affect insect gut bacterial communities (12).…”

Section: Figmentioning

confidence: 99%

Insect Gut Bacterial Diversity Determined by Environmental Habitat, Diet, Developmental Stage, and Phylogeny of Host

Yun

Roh

Whon

et al. 2014

Appl Environ Microbiol

644

554

View full text Add to dashboard Cite

dInsects are the most abundant animals on Earth, and the microbiota within their guts play important roles by engaging in beneficial and pathological interactions with these hosts. In this study, we comprehensively characterized insect-associated gut bacteria of 305 individuals belonging to 218 species in 21 taxonomic orders, using 454 pyrosequencing of 16S rRNA genes. In total, 174,374 sequence reads were obtained, identifying 9,301 bacterial operational taxonomic units (OTUs) at the 3% distance level from all samples, with an average of 84.3 (؎97.7) OTUs per sample. The insect gut microbiota were dominated by Proteobacteria (62.1% of the total reads, including 14.1% Wolbachia sequences) and Firmicutes (20.7%). Significant differences were found in the relative abundances of anaerobes in insects and were classified according to the criteria of host environmental habitat, diet, developmental stage, and phylogeny. Gut bacterial diversity was significantly higher in omnivorous insects than in stenophagous (carnivorous and herbivorous) insects. This insect-order-spanning investigation of the gut microbiota provides insights into the relationships between insects and their gut bacterial communities.

show abstract

Parallel metatranscriptome analyses of host and symbiont gene expression in the gut of the termite Reticulitermes flavipes

Cited by 205 publications

References 96 publications

Diversity and Genomes of Uncultured Microbial Symbionts in the Termite Gut

Diversity and Genomes of Uncultured Microbial Symbionts in the Termite Gut

Metagenomic Analyses: Past and Future Trends

Insect Gut Bacterial Diversity Determined by Environmental Habitat, Diet, Developmental Stage, and Phylogeny of Host

Contact Info

Product

Resources

About