Parallel synthesis of libraries of anodic and cathodic functionalized electrodeposition paints as immobilization matrix for amperometric biosensors

Membrane‐Less Biofuel Cell Based on Cellobiose Dehydrogenase (Anode)/Laccase (Cathode) WiredviaSpecific Os‐Redox Polymers

“…Copolymers were synthesised by free-radical polymerisation [55], and the obtained product was neutralised using NaOH. Water-methanol suspensions of the Os-complexmodified electrodeposition paints (EDPs) with defined solid content (10-15%) were obtained by ligand-exchange reaction between a labile chloro ligand of Osmium-bis-(2,2′-bipyridyl)-dichloride and functional groups (imidazolyl-or pyridyl groups) at the polymer backbone followed by the removal of methanol from the solution.…”

“…The syntheses of heterocyclic imidazolyl-functionalised monomers were carried out as previously reported [55][56][57]. Poly-mers A186-A440 were obtained by varying the type and ratio of the monomers during the radical polymerisation.…”

“…Redox polymers containing Os-complex were in-house synthesised as previously described [55], and utilised as suspensions in water with a concentration of about 10-15%. The syntheses of heterocyclic imidazolyl-functionalised monomers were carried out as previously reported [55][56][57].…”

Design of a bioelectrocatalytic electrode interface for oxygen reduction in biofuel cells based on a specifically adapted Os-complex containing redox polymer with entrapped Trametes hirsuta laccase

“…Advantages arising from integrating the biocatalysts within three-dimensional Os-complex modified polymer layers are the significantly higher amount of the biocatalyst which is electrically connected via the polymer-bound redox centres to the electrode [17]. Recently, we have introduced a strategy for synthesizing Os-complex modified electrodeposition polymers [18][19][20]. Using a number of different monomers for the radical polymerization in combination with different Os-complexes, which are tethered to the polymer backbone, not only the formal potentials of the polymer-bound redox relays but also the physical properties (e.g.…”

Amperometric Enzyme-based Biosensors for Lowering the Interferences

“…In the following decades, the immobilization of enzyme became a key issue in developing the enzyme-based biosensor. Generally speaking, the immobilized methods (Cunningham, 1998) of enzyme include adsorption (Chu et al, 2007;Ekanayake et al, 2007), entrapment (Ngounou et al, 2007;Seo et al, 2007), cross-linking (Akyilmaz & Yorganci, 2007) and covalent bonding (Lin et al, 2007a;Seo et al, 2007). For adsorption, the enzyme was attached on the electrode by the attractive force of hydrogen bonds or opposite charges, such as nylon (Gamati et al, 1991) and ion exchange resin (Zhujun & Seitz, 1986), but it did not form a good adhesive force between biomolecules and a transducer.…”