Paralytic and insecticidal toxins from the funnel web spider, Hololena curta

Quistad, Gary B.; Reuter, Carol C.; Skinner, Wayne S.; Dennis, Paula A.; Suwanrumpha, Somchai; Fu, Emil W.

doi:10.1016/0041-0101(91)90286-z

Cited by 60 publications

(28 citation statements)

References 13 publications

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“…Data from the literature, [27][28][29] as well as our own experience with RP-HPLC analysis of various tarantula venoms, 30 suggest that early eluting peaks in reversedphase systems are characteristic of low-molecular weight compounds such as biogenic amines and polyamines. Compounds eluting in the 30-50 minutes range (35-55% acetonitrile) are probably peptides with molecular masses in the range 2000-8000 Da and lateeluting compounds may represent components of higher molecular mass ( > 10 000 Da).…”

Section: Resultsmentioning

confidence: 95%

High-performance liquid chromatography matrix-assisted laser desorption/ionization time-of-flight mass spectrometry peptide fingerprinting of tarantula venoms in the genusBrachypelma: chemotaxonomic and biochemical applications

Escoubas

Célérier

Nakajima

1997

Rapid Commun. Mass Spectrom.

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Precise identification of arthropod species is fundamental in venom research, particularly in groups where taxonomy problems remain unsolved. High-performance liquid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) analysis of crude venoms of six tarantula species in the genus Brachypelma showed that the characteristic chromatographic and peptide ion profiles obtained can be used to discriminate amongst closely related species. This method permits rapid mass fingerprinting of large numbers of samples in a reproducible manner, and offers a powerful systematic tool in combination with morphological methods for the classification of tarantula species. The sensitivity and precision of the method may offer a way to solve complex taxonomic relationships not easily resolved by morphological measurements, in a non-destructive manner. Additionally, peptide mapping of crude venoms by MALDI-TOFMS will speed up the discovery of novel ligands of neuronal receptors, since major venom components of related species share a high sequence homology and are likely to possess similar pharmacological properties.

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Section: Resultsmentioning

confidence: 95%

High-performance liquid chromatography matrix-assisted laser desorption/ionization time-of-flight mass spectrometry peptide fingerprinting of tarantula venoms in the genusBrachypelma: chemotaxonomic and biochemical applications

Escoubas

Célérier

Nakajima

1997

Rapid Commun. Mass Spectrom.

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“…These toxins obviously recognize a common conserved pattern present on the channels. Some toxins, such as ω-atracotoxins from Hadronyche species [33,34,35,36], toxins from Hololena curta [37,38] and Plectreurys toxin II (PLTX-II) [39], modulate insect voltage-gated calcium channels and are useful in research of novel insecticides, while other toxins have interesting, more or less specific, activities on mammalian voltage-gated calcium channels. Hololena curta venom, in addition to it insecticidal activity, is a potent inhibitor of voltage sensitive calcium channels and neurotransmitter release in mammalian brain synaptosomes [40].…”

Section: Peptide Modulators Of Voltage-gated Calcium Channelsmentioning

confidence: 99%

Peptide Neurotoxins That Affect Voltage-Gated Calcium Channels: A Close-Up on ω-Agatoxins

Pringos

Vignes

Martinez

et al. 2011

Toxins

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Peptide neurotoxins found in animal venoms have gained great interest in the field of neurotransmission. As they are high affinity ligands for calcium, potassium and sodium channels, they have become useful tools for studying channel structure and activity. Peptide neurotoxins represent the clinical potential of ion-channel modulators across several therapeutic fields, especially in developing new strategies for treatment of ion channel-related diseases. The aim of this review is to overview the latest updates in the domain of peptide neurotoxins that affect voltage-gated calcium channels, with a special focus on ω-agatoxins.

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“…most abundant polyamin toxins of the spiders Agelenopsis aperta 7 and Hololena curta. 8 In the meantime, many different spider species have been investigated by several groups and the analytical procedures have been well refined.…”

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confidence: 99%

A Template Approach for the Characterization of Linear Polyamines and Derivatives in Spider Venom

Tzouros

Chesnov

Bigler

et al. 2013

Eur J Mass Spectrom (Chichester)

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A combination of high-performance liquid chromatography (HPLC) and atmospheric-pressure chemical ionization mass spectrometry (APCI-MS and APCI-MS/MS) was used to detect and characterize linear polyamine derivatives in the venom of the spiders Agelenopsis aperta, Hololena curta and Paracoelotes birulai. The compounds were identified with a template approach, by which the collision-induced dissociation (CID) spectra of known compounds are directly compared and correlated with those of the analytes. To facilitate the perception of the spectra and the recognition of the structural features of the analytes, an ion nomenclature closely leaned on the accepted nomenclature for fragment ions of peptides or nucleic acids is introduced. The structure identification of polyamine derivatives by direct correlation of MS spectra is possible because such compounds show very distinctive fragmentation behavior. Of particular relevance is the fact that the signal patterns that are observed with analytes possessing different polyamine backbones are not only distinct with regard to mass distributions but also with regard to relative signal intensities, resulting in fingerprint-like signal patterns. The direct correlation of these patterns--more than the correlation of the ion distributions--was found to be of key significance. With this, the new approach is fundamentally different from the sequencing of peptides or nucleic acids, which are largely based on mass distributions. The method is more efficient and more reliable than the de novo interpretation of the MS data and it even allows the identification of polyamine portions in compounds that are analyzed within mixtures.

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Paralytic and insecticidal toxins from the funnel web spider, Hololena curta

Cited by 60 publications

References 13 publications

High-performance liquid chromatography matrix-assisted laser desorption/ionization time-of-flight mass spectrometry peptide fingerprinting of tarantula venoms in the genusBrachypelma: chemotaxonomic and biochemical applications

High-performance liquid chromatography matrix-assisted laser desorption/ionization time-of-flight mass spectrometry peptide fingerprinting of tarantula venoms in the genusBrachypelma: chemotaxonomic and biochemical applications

Peptide Neurotoxins That Affect Voltage-Gated Calcium Channels: A Close-Up on ω-Agatoxins

A Template Approach for the Characterization of Linear Polyamines and Derivatives in Spider Venom

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