Parameters affecting cellular adhesion to polylactide films

Abstract

Absorbable biomaterials have been recently incorporated into the field of tissue engineering. Little work has been performed, even with the clinically acceptable absorbables, concerning their tissue promoting capability or lack, thereof. Furthermore, the relative attractions of cells to these implants may be largely disguised by the presence of serum. This research involved the development of an adhesion assay to compare the adhesion behavior of two cell types to two different polylactides in a serum free environment. The results showed that the attachment behavior depends not only on the cell or the polymer but a combination of the two.

Application of magnetic resonance microscopy to tissue engineering: A polylactide model

“…The static loading was achieved by placing the constructs in individual wells of a six‐well tissue culture plate and pipeting a specific quantity of cells and 250 μL of media onto each porous construct, the amount of media corresponding to the estimated pore volume. After 24 h, the time considered adequate for cellular attachment,26 5 mL of supplemented SMGM (denoted SMGM S ) was added to each well. The dynamic seeding was attained by placing the polymers and cells into 250‐mL stir flasks with 20‐mL of media per polymer, four polymers per flask, and stirring at 24 rpm.…”

Physical and mechanical properties of PLA, and their functions in widespread applications — A comprehensive review

“…3) Hydrophobicity -PLA is relatively hydrophobic, with a static water contact angle of approximately 80°C. This results in low cell affinity, and can elicit, in some cases, an inflammatory response from the living host upon direct contact with biological fluids [22]. 4) Lack of reactive side-chain groups -PLA is chemically inert with no reactive side-chain groups making its surface and bulk modifications a challenging task.…”

Tissue Engineering Generation of adipose tissue: an overview of current standards and possibilities

“…Advantages of synthetic scaffolds are the uniform production under controlled conditions, and the ability of modifying their chemical and physical properties according to specific needs [18,25,26]. The advantage of natural matrices is their less frequent cause of foreign body reactions.…”

“…When attempting to restore the original shape of the body, a matrix should be so pliable that it can assume the intended shape and texture of the recipient's own adipose tissue. In an ideal setting, the matrix acquires the desired shape and maintains its form while being absorbed during the process of target tissue generation [25]. The available forms of matrices so far being used range from porous sponges and plates to polymeric cylinders.…”