Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Muijselaar, Pim G.; Claessens, H.A.; Cramers, C.A.M.G.

doi:10.1016/0021-9673(94)01293-n

Cited by 54 publications

(37 citation statements)

References 25 publications

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“…In MEKC, there is a relationship between the EOF velocity and the organic modifier concentration, due to viscosity modulation of EOF by the organic modifier [6]. Additionally, it is possible to influence the partitioning equilibrium by adding organic solvents.…”

Section: Effect Of Concentration Of the Organic Modifiermentioning

confidence: 99%

Separation of cold medicine ingredients using a precise MEKC method at elevated pH

Hasemann¹,

Balk²,

Preu³

et al. 2007

Electrophoresis

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Separation of cold medicine ingredients using a precise MEKC method at elevated pHAn MEKC method was developed in order to separate a cold medicine formulation containing acetaminophen, ephedrine sulfate, doxylamine succinate, and dextromethorphan hydrobromide as active pharmaceutical ingredients. Because of their similar physical and chemical properties, it was a challenge to separate the basic compounds without sample pretreatment. In addition, the high content of alcohol and sucrose together with the variety of further excipients had to be considered. Thus, the complex matrix required several optimization steps. These included the search for the optimum pH and for a suitable sodium dodecyl sulfate concentration to avoid matrix-capillary wall interaction and to ensure precision. As a second developing step, an internal standard (benzocaine) was chosen to guarantee a high level of quantitative performance. An RSD% value of the peak areas between 1.0 and 2.0 was reached. The employed method development strategy can be generalized to similar separation approaches in the future.

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Section: Effect Of Concentration Of the Organic Modifiermentioning

confidence: 99%

Separation of cold medicine ingredients using a precise MEKC method at elevated pH

Hasemann¹,

Balk²,

Preu³

et al. 2007

Electrophoresis

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“…An overview was given by Muijselaar et al [2]. These solutions offer a reasonable enlargement of the elution window for most applications, but increase the complexity of the MEKC system.…”

Section: Introductionmentioning

confidence: 98%

Experiments with Organic Modifiers in Reversed-Flow MEKC

Vercammen

Verleysen

Sandra

1998

J. High Resol. Chromatogr.

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“…In recent years, the presence of alcohols of different chain lengths and concentrations in the pseudostationary phase has been reported to have profound effects on both chiral [8][9][10] and achiral [11][12][13] separations. The presence of organic modifiers in the pseudostationary phase alters thermodynamic factors and electrokinetic behavior such as solute retention and electroosmotic migrations, respectively.…”

Section: Introductionmentioning

confidence: 99%

Enantioselectivity of alcohol‐modified polymeric surfactants in micellar electrokinetic chromatography

et al. 2003

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A novel method of modifying sodium undecanoyl-L-leucinate (SUL) micelles employed in chiral separation of analytes in micellar electrokinetic chromatography (MEKC) to enhance selectivity toward specific analytes is discussed. The current study aimed at modifying the SUL micelles by introducing different alcohols into the mono-SUL micelles. The micellar solutions were then polymerized in the presence of alcohols followed by postpolymerization extraction of the alcohols to yield alcohol-free polymeric surfactants (poly-L-SUL). The effects of hexanol (C(6)OH) and undecylenyl alcohol (C(11)OH) on micellar properties of this surfactant were investigated by use of surface tensiometry, fluorescence spectroscopy, pulsed field gradient-nuclear magnetic resonance (PFG-NMR), and MEKC. The surface tension and PFG-NMR studies indicated an increase in the critical micelle concentration (cmc) and micellar size upon increasing the alcohol concentration. Fluorescence measurements suggested that alcohols induce closely packed micellar structures. Coumarinic and benzoin derivatives, as well as (+/-)-1, 1'-binaphthyl-2,2'-dihydrogen phosphate (BNP) were used as test analytes for MEKC experiments. Examination of MEKC data showed remarkable resolutions and capacity factors of coumarinic derivatives obtained with modified poly-L-SUL as compared to the unmodified poly-L-SUL. Evaluation of fluorescence, PFG-NMR, and MEKC data suggest a strong correlation between the polarity and hydrodynamic radii of alcohol-modified micelles and the resolution of the test analytes.

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Parameters controlling the elution window and retention factors in micellar electrokinetic capillary chromatography

Cited by 54 publications

References 25 publications

Separation of cold medicine ingredients using a precise MEKC method at elevated pH

Separation of cold medicine ingredients using a precise MEKC method at elevated pH

Experiments with Organic Modifiers in Reversed-Flow MEKC

Enantioselectivity of alcohol‐modified polymeric surfactants in micellar electrokinetic chromatography

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