Parameters of Cellular Immunity in Primates

Ignatova, I. E.; Agrba, V. Z.

doi:10.1007/s10517-010-0882-7

Cited by 5 publications

(4 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The choice of this monkey species (Macaca fascicularis) was explained by its small size, no need in special ration, and because the vaccines protecting from human infections are created with the use of this very species. We previously studied standard values of Macaca rhesus (the species most often used in experiments) [3].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Cellular Immunity Standard Values in Macaca Fascicularis

2014

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

“…The study was carried out by our method described previously [3]. Flow laser cytometry was carried out on an Epics XL-MCL device (Beckman Coulter) with argon laser (λ=488 nm).…”

Section: Methodsmentioning

confidence: 99%

Cellular Immunity Standard Values in Macaca Fascicularis

2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lysing solution (2 ml) was added into the sample which was then incubated (10-15 min) in the darkness at ambient temperature till the sample clarifi cation. Two washing procedures were then carried out by the standard method [3]. The samples were analyzed on the same day.…”

Section: Methodsmentioning

confidence: 99%

Effects of Low-Dose γ-Irradiation on the Counts of Immunocompetent Cells in a Model Experiment on Primates

2010

Self Cite

View full text Add to dashboard Cite

Cellular immunity was studied by flow cytometry with Becton Dickinson monoclonal antibodies in clinically healthy Macaca mulatta males before and after low-dose exposure to ionizing radiation. It was shown that T and B cells are radiosensitive, B cells being more sensitive, which is seen from a significant drop of their count. Natural killers are radioresistant. The count of immunocompetent cells recovers sooner after single compared to fractionated irradiation in the same summary dose.

show abstract

“…данные по содержанию общих т-и в-лимфоцитов макак-резусов, полученные с использованием комбинации маркеров сD2 и сD20, согласуются с результатами иммунофенотипирования при использовании маркеров сD3 и сD19 или сD3 и сD20. данные по содержанию T-хелперов и цитотоксических T-лимфоцитов макак-резусов, полученные с использованием комбинации маркеров сD2, сD4 и сD8, согласуются с результатами, полученными с использованием комбинации маркеров сD3, сD4 и сD8 [7,10].…”

Section: материалы и методыunclassified

Analysis of Cellular Component Indicators of Immune Status of Rhesus Macaques

Борисевич¹,

Кириллова²,

Сидорова³

et al. 2021

Problemy Osobo Opasnykh Infektsii

View full text Add to dashboard Cite

Objective. Selection of indicators of lymphocyte populations in rhesus macaques determined by flow cytometry to evaluate variations of cellular constituent of their immune status.Materials and methods. Blood of 11 healthy rhesus macaque males, 2,0–2,5 years old, weighing 2,5–3,0 kg, was used. Monkeys were examined simultaneously in each of 7 months of observation (since May till November). Immunophenotyping was conducted by FC500 cytofluorimeter using Affymetrix eBioscience monoclonal antibodies. The following cellular constituent indicators of immune status were differentiated: total T lymphocytes (phenotype CD2+CD20-); total B lymphocytes (phenotype CD2-CD20+); natural killer cells (phenotype CD2+CD56+); T helper cells (phenotype CD2+CD4+); cytotoxic T lymphocytes (phenotype CD2+CD8+); double-positive T lymphocytes (phenotype CD4+CD8+) and T lymphocytes positive by antigens CD2 and CD20 (phenotype CD2+CD20+).Results and discussion. Statistical analysis of the obtained results revealed the absence of the effect of research time factor on the stated indicators. To assess changes in the cellular constituent of immune status of rhesus macaques, it is possible to use indicators that are less variable: total T and B lymphocytes, T helper cells, cytotoxic T lymphocytes and T lymphocytes with phenotype (CD2+CD20+). The use of CD56 as a marker of natural killer cells of rhesus macaques is impractical due to its low expression and a small size of the population bearing this marker. The research results may form the basis of the normative indicators of the subpopulation cell composition of immune system in rhesus macaques, which will allow the study of infected animals when assessing the quality of medical products in relation to dangerous and particularly dangerous infections.

show abstract

Parameters of Cellular Immunity in Primates

Abstract: Cellular immunity in clinically healthy Macaca mulatta was studied by flow cytometry using monoclonal antibodies (Becton Dickinson). The immune parameters of monkeys were close to those in humans.

Cited by 5 publications

References 1 publication

Cellular Immunity Standard Values in Macaca Fascicularis

Cellular Immunity Standard Values in Macaca Fascicularis

Effects of Low-Dose γ-Irradiation on the Counts of Immunocompetent Cells in a Model Experiment on Primates

Analysis of Cellular Component Indicators of Immune Status of Rhesus Macaques

Contact Info

Product

Resources

About