Parametric evaluation of flux decline during ultrafiltration of protein solutions

Abulnour, A.G.; Talaat, H.A.; Sorour, Mohamed H.; Tewfik, Shadia R.

doi:10.1016/0011-9164(88)80040-7

Cited by 6 publications

(5 citation statements)

References 3 publications

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“…Interestingly, fouling problems caused by irreversible pore plugging and/or protein adsorption inside pores (R ir ) are 34% greater for the ultrafilter. We speculated that the deposition of proteins within the pores resulted in significant loss in convective flow, a phenomenon well documented for ultrafiltration (Abilnaour et al, 1988;Chudacek and Fane, 1984;Kim et al, 1992;Koltuniewicz, 1992;Nilsson, 1980) and reported recently for microfiltration membranes (Bowen and Gan, 1991;Bowen et al, 1995). However, the role of soybean oil as well as numerous surface-active agents present in the whole broth was not clear, except that early breakthrough assisted by surfactants (Vaidya et al, 1994b) certainly did not occur here.…”

Section: Membrane Foulingmentioning

confidence: 58%

Two-phase bioconversion product recovery by microfiltration I. Steady state studies

Conrad

Lee

1998

Biotechnol. Bioeng.

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Recovery of an aqueous bioconversion product from complex, two‐phase Pseudomonas putida broths containing 20% (v/v) soybean oil presents a significant challenge for downstream processing. Although not used before in multiple‐phase separation for complex biotech products, crossflow filtration employing ceramic filters is one of the most attractive options which allow the design of integrated, continuous bioconversion processes. As a first attempt, we studied multichannel, monolithic ceramic membranes of different nominal pore sizes and lumen diameters under steady‐state conditions. The best performance was obtained with 0.2‐μm‐pore/3‐mm‐lumen membrane, which completely rejected both cells and oil droplets from the permeate, creating a clear aqueous product stream. Although the same separation was achieved, the 50K molecular weight cut‐off (MWCO) ultrafilter showed greater irreversible but similar reversible resistance, in addition to an order‐of‐magnitude higher membrane resistance. Larger nominal pore microfilters, such as 0.45 and 1.0 μm, experienced both cell and oil leakage even at low transmembrane pressure (10 psig). Attributed to greater shear at the same recirculation rate, smaller lumen filters did provide greater permeate flux. However, for practical purposes, the 0.2‐μm‐pore/4‐mm‐lumen ceramic membrane was chosen for further evaluation. Transmembrane pressures up to 50 psig provided only marginal gains in filtration performance, whereas increasing shear rate resulted in linear increases in steady‐state flux, presumably due to formation of shear‐sensitive, complex gel/oil/cell layer near the membrane surface. A nominal shear rate of 9200 s−1 and 20 psig transmembrane pressure were chosen as optimal operating conditions. Additional studies in a clean system revealed that as low as 5% (v/v) soybean oil in deionized (DI) water resulted in an order‐of‐magnitude decline in steady‐state permeate flux. Breakthrough of oil droplets occurred at 35 psig transmembrane pressure. The severe fouling and breakthrough phenomena disappeared in the presence of washed cells for transmembrane pressure up to 43 psig, implying an oil/cell layer coating the membrane surface, thus preventing oil penetration. Serious membrane fouling was also experienced in microfiltration of oil‐free, cell‐free supernatant and oil‐free whole broth. Consequently, soluble proteins/surfactants were suspected to be the major membrane foulants. Interestingly, soybean oil up to 30% (v/v) enhanced the flux, presumably through complicated interactions with the major foulants. Regeneration of membrane was best achieved with protease and hot caustic/bleach treatments, supporting the hypothesized fouling mechanisms mentioned above. This work provides process and system information for batch microfiltration runs in the future, to be reported elsewhere as Part II of this work. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 631–641, 1998

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Section: Membrane Foulingmentioning

confidence: 58%

Two-phase bioconversion product recovery by microfiltration I. Steady state studies

Conrad

Lee

1998

Biotechnol. Bioeng.

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“…[3] can be converted to a standard transform (27 and 28) if the Langmuir adsorption model is used for m(χ; G ads ). Therefore, [4] where M p is the molecular mass of the protein and N is the number of adsorption sites per surface unit. Then, if the following variables are defined,…”

Section: Appendix A: Freundlich's Isothermmentioning

confidence: 99%

Protein Adsorption and Deposition onto Microfiltration Membranes: The Role of Solute–Solid Interactions

Martínez

Martin

Prádanos

et al. 2000

Journal of Colloid and Interface Science

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“…Depending on experimental conditions, runs lasted 100-400 min. The total resistance (R,) was calculated with the stabilized permeate flow rate using the equation RT=APT/(q*J) (Chiang and Cheryan, 1986;Abulnour et al, 1988). The membrane was rinsed with distilled water immediately after each run, and water flow rates under the processing conditions were measured to estimate irreversible fouling (R3 of the membrane (due to a layer that could not be removed with water) using again the equation Rt= APT/(q*J)-Rm.…”

Section: Juice Filtrationmentioning

confidence: 99%

Optimization and Modeling of Apple Juice Cross‐flow Microfiltration with a Ceramic Membrane

Qiu

McLELLAN

1993

Journal of Food Science

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The performance of a 0.2 km ceramic membrane for clarification of depectinized apple juice was studied. The results showed that the flux was higher at high feed velocities (14.6 m/set) and high temperatures (5o"C), and the transmembrane pressure was a positive factor only at high temperatures. The juice flux at optimal conditions was between 4&J-500'kg/hr.m*. Filtration of juice with pectin resulted in flux decreases of 40-50% compared to deoectinized juice. Periodic backflushing during processing' at optimal conditions, i.e., high temperature, high feed velocity and low pressure, did not significantly increase the juice flux.

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Parametric evaluation of flux decline during ultrafiltration of protein solutions

Cited by 6 publications

References 3 publications

Two-phase bioconversion product recovery by microfiltration I. Steady state studies

Two-phase bioconversion product recovery by microfiltration I. Steady state studies

Protein Adsorption and Deposition onto Microfiltration Membranes: The Role of Solute–Solid Interactions

Optimization and Modeling of Apple Juice Cross‐flow Microfiltration with a Ceramic Membrane

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