A monocopter, which is a biology-inspired aircraft based on the samara, has been proved to have passive flight stability. However, due to the asymmetry of its configurations and the constant rotation during flight, its flight dynamics equation is complex. In this paper, the longitudinal stability of a monocopter is systematically analyzed. The longitudinal motion of the monocopter is used as the main research object in this paper. By transforming the body axis coordinate frame to the semi-body axis coordinate frame, its longitudinal dynamics equation is greatly simplified. Then, a fourth-order state space matrix of the longitudinal motion of the monocopter is established, and its longitudinal stability is analyzed at different pitch angles of the wing. Furthermore, the fourth-order state space matrix is simplified into a third-order matrix, and the Rouse criterion is used to analyze the stability of the simplified state space. A set of sufficient conditions is obtained as the criterion for longitudinal stability. Based on this criterion, it is found that the product of inertia [Formula: see text] is a very important factor affecting the stability. Then, the inertial parameters of the aircraft are modified, which greatly expands the range of the pitch angle that maintains longitudinal stability. Finally, the six-degree-of-freedom nonlinear flight dynamic simulation is performed to verify the rationality of the longitudinal stability criterion.