ParaSight®F Test Compared with the Polymerase Chain Reaction and Microscopy for the Diagnosis of Plasmodium falciparum Malaria in Travelers

Humar, Atul; Ohrt, Colin; Harrington, Mary Anne; Pillai, Dylan R.; Kain, Kevin C.

doi:10.4269/ajtmh.1997.56.44

Cited by 194 publications

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“…11 In previous trials of patients from areas of transmission of P. falciparum, this test has generally demonstrated high sensitivity and specificity when compared with light microscopy, the current gold standard for diagnosing malaria. [12][13][14][15][16][17][18][19][20][21][22][23][24] High sensitivity and specificity values have also been documented for detecting P. falciparum malaria in areas of mixed P. falciparum/P. vivax transmission in Brazil (91% and 97%, respectively), Sri Lanka (90.2% and 99.1%, respectively), and Sumba Island in eastern Indonesia, (95.5% and 89.8%, respectively).…”

Section: Introductionmentioning

confidence: 99%

Assessing the Parasight-F test in northeastern Papua, Indonesia, an area of mixed Plasmodium falciparum and Plasmodium vivax transmission.

Taylor¹,

Widjaja²,

Basri³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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Abstract. User-friendly, reliable, and inexpensive methods for diagnosing malaria are needed at the primary health care level. During a randomized treatment trial, the Parasight-F test was assessed on days 0, 3, 7, and 28 against standard light microscopy of Giemsa-stained thick blood smears for diagnosing Plasmodium falciparum parasitemia in patients with P. falciparum (n ‫ס‬ 84) or P. vivax (n ‫ס‬ 59) malaria. The median P. falciparum parasite count on day 0 was 2,373/L (range ‫ס‬ 20-74,432/L). At the start of treatment, the Parasight-F test had a sensitivity of 95.2% (80 of 84; 95% confidence interval [CI] ‫ס‬ 88.2-98.7), and a specificity of 94.9% (56 of 59; 95% CI ‫ס‬ 85.8-98.9). On day 7, this test showed false-positive results in 17 (16.3%) of 104 patients (95% CI ‫ס‬ 9.8-24.9). The Parasight-F test performed well when compared with light microscopy in detecting P. falciparum parasitemia in patients presenting with clinical malaria. However, the high false-positive rate on day 7 limits its use for patient follow-up.

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Section: Introductionmentioning

confidence: 99%

Assessing the Parasight-F test in northeastern Papua, Indonesia, an area of mixed Plasmodium falciparum and Plasmodium vivax transmission.

Taylor¹,

Widjaja²,

Basri³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

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“…2 There are only limited data on posttreatment persistence of HRP2 antigenemia with the immunoglobulin (Ig) M monoclonal antibody to HRP2 used in the ICT Pf 6 and Pf/Pv tests, 16,18,20 but these suggest rates of persistence at least as high as those found with the IgG HRP2 monoclonal antibody used in the ParaSight-F test. [3][4][5][7][8][9][10]13,14 In the Sumbanese population described above, we found persistence of HRP2 in 29 and 10% of those negative for asexual parasites by microscopy on Days 7 and 14, respectively. 20 The causes of posttreatment persistence of HRP2 with both the ICT test and ParaSight-F tests are unclear; they may vary with drug treatment used and geographic region.…”

Section: Discussionmentioning

confidence: 49%

“…2 However, there are only limited data on the ability of antigen detection tests to detect and predict TF when used during or after the completion of therapy. Histidine rich protein 2 (HRP2) antigen is known to persist after resolution of clinical symptoms and clearance of viable asexual parasitemia, [3][4][5][6][7][8][9][10][11][12][13][14] which may limit its ability to reliably predict recrudescent parasitemia. Although some studies have suggested rapid antigen detection tests may be potentially useful for monitoring the response to antimalarial therapy or predicting TF, [7][8][9]12,15 concern has been raised by other studies that persistence of HRP2 antigen after clearance of parasitemia may lead to confusing results when used for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Detection of histidine rich protein 2 and panmalarial ICT Malaria Pf/Pv test antigens after chloroquine treatment of uncomplicated falciparum malaria does not reliably predict treatment outcome in eastern Indonesia.

Tjitra

Suprianto

Dyer³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. In regions with drug-resistant malaria, the ability to rapidly detect or predict treatment failure (TF) soon after a course of standard therapy for Plasmodium falciparum malaria would facilitate the prompt institution of secondline therapy. We thus evaluated longitudinally the ability of the ICT Malaria Pf/Pv immunochromatographic test to predict treatment outcome. Sixty-six Sumbanese Indonesians with uncomplicated falciparum malaria were treated with chloroquine and followed for 28 days by use of 1997 World Health Organization criteria for assessment of therapeutic efficacy of antimalarial drugs. The ICT Pf/Pv testing could be compared with microscopy in approximately half of the patients on each day of follow-up. Although strongly positive histidine rich protein 2 (HRP2) line intensities (equal to or greater than the control band) in convalescence were highly predictive of TF, any degree of positivity for the HRP2 and panmalarial antigens in convalescence was only moderately predictive of TF. Positive predictive values of the HRP2 and panmalarial antigens for TF were 76.9% and 87.0%, respectively, on Day 3, 82.4% and 87.5% on Day 7, and 78.9% and 78.9% on Day 14. Negative HRP2 and panmalarial antigen results in convalescence were even less predictive of an adequate clinical response, and false-negative HRP2 and panmalarial antigen test results were found in one-sixth (6 of 37) of recrudescent infections diagnosed by microscopy among patients with late treatment failure. To reliably predict treatment outcome with rapid antigen tests, further development appears necessary to improve sensitivity for viable asexual parasites while avoiding detection of both gametocytes and persistent antigen in convalescence.

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“…También puede realizarse el diagnóstico por la detección del DNA del parásito, mediante técnicas de PCR, que es un método muy sensible, pero que no se encuentra a la disposición de muchos centros hospitalarios (8).…”

Section: Discussionunclassified

Cuatro casos de paludismo en viajeros: Una enfermedad emergente

Sánchez

Castaño

Blanco

et al. 2007

An. Med. Interna (Madrid)

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INTRODUCCIÓNEl paludismo o malaria es una infección producida por protozoos intracelulares del género Plasmodium. Existen cuatro especies: Plamodium vivax, P. malariae, P. ovale y Plasmodium falciparum.La enfermedad se transmite a través de la picadura del mosquito Anopheles.Constituye un grave problema de salud pública, al causar de 300 a 500 millones de casos al año (1).El continente más afectado es África, fundamentalmente en los países al sur del Sahara.España fue declarada zona libre de malaria en el 1964, pero después de unos años en los que apenas se declararon algunos casos, el diagnóstico de malaria vuelve a ser relativamente frecuente en nuestro país, debido al incremento de los viajes por turismo, negocios, trabajos diversos de cooperación y sobre todo en relación con migraciones.En Europa los casos importados oscilan entre 13.000 y 16.000 por año, con una mortalidad del 2-3% (2).En España se declaran unos 400 casos anuales de paludismo importado, la mayor parte de los mismos en la Comunidad Autónoma de Madrid (3).En Europa también se han detectado casos del denominado paludismo de aeropuerto, que se trata de casos de malaria diagnosticados en personas que no han viajado y que han sido picados por el mosquito cerca de los aeropuertos o bien por mosquitos infectados transportados por aviones procedentes de países endémicos o bien paludismo de las maletas, producido por mosquitos infectados dentro de las maletas de los viajeros que vienen de países endémicos y que son liberados al abrirse las maletas en el país de destino (4). [0212-7199 (2007) RESUMENPresentamos cuatro casos de paludismo en pacientes que habían viajado en las semanas previas a Guinea Ecuatorial, sin haber realizado profilaxis. Eran 3 varones y una mujer, dos naturales de Guinea Ecuatorial, pero residentes en España desde hace largo tiempo y otros dos de nuestro país. Tras realizar un viaje de una duración entre 15 y 30 días por su país, presentaron entre los días 5 y 10 de su regreso, un cuadro de fiebre, escalofríos, cefalea y en dos casos diarreas. Se palpó esplenomegalia en un solo paciente. Se observaron plasmodium en la extensión de sangre periférica en los cuatro enfermos, identificándose Plasmodium falciparum, en dos pacientes. Todos fueron tratados con sulfato de quinina y doxiciclina, evolucionando favorablemente. Un paciente fue tratado en régimen de hospitalización. Posteriormente comentamos algunos aspectos epidemiológicos, clínicos, diagnósticos y de tratamiento de esta enfermedad emergente.PALABRAS CLAVES: Paludismo. Plasmodium. Extensión de sangre periférica. Gota gruesa. ABSTRACT Four cases of malaria in patients who had travelled to Equatorial Gui

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ParaSight®F Test Compared with the Polymerase Chain Reaction and Microscopy for the Diagnosis of Plasmodium falciparum Malaria in Travelers

Cited by 194 publications

References 0 publications

Assessing the Parasight-F test in northeastern Papua, Indonesia, an area of mixed Plasmodium falciparum and Plasmodium vivax transmission.

Assessing the Parasight-F test in northeastern Papua, Indonesia, an area of mixed Plasmodium falciparum and Plasmodium vivax transmission.

Detection of histidine rich protein 2 and panmalarial ICT Malaria Pf/Pv test antigens after chloroquine treatment of uncomplicated falciparum malaria does not reliably predict treatment outcome in eastern Indonesia.

Cuatro casos de paludismo en viajeros: Una enfermedad emergente

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