Parasitic load determination by differential expressions of 5-lipoxygenase and PGE2 synthases in visceral leishmaniasis

Saini, Sharanjot; Singh, Bharat; Prakash, Satya; Kumari, Smita; Kureel, Amit Kumar; Dube, Anuradha; Sahasrabuddhe, Amogh A.; Kumar, Ambak

doi:10.1016/j.prostaglandins.2019.106390

Cited by 11 publications

(11 citation statements)

References 34 publications

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“…AA gives rise to various bioactive molecules e.g., prostaglandins (PGs) and leukotrienes (LTs). Various groups have emphasized on roles of PGs and LTs in the immune response against Leishmania infection (Morato et al, 2014 ; Saha et al, 2014 ; Chaves et al, 2016 ; Saini et al, 2019 ). However, the possibilities of their therapeutic applications are limited, as these molecules are transient in nature as well as not cost-effective.…”

Section: Discussionmentioning

confidence: 99%

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Linoleic Acid Inhibits the Release of Leishmania donovani Derived Microvesicles and Decreases Its Survival in Macrophages

Saini¹,

Kumar²

2020

Front. Cell. Infect. Microbiol.

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Visceral leishmaniasis is a neglected tropical disease caused by Leishmania (L.) donovani parasite in the Indian subcontinent. Macrophages (mϕ) are the harboring cells for parasite and their interactions dictate the pathogenesis of this disease. Polyunsaturated fatty acids are an integral part of the mϕ cell membrane and are derived from linoleic acid (LA), which is a principal essential fatty acid. Here, we have investigated the effect of the simultaneous presence of LA during L. donovani infection in mϕ. Treatment with LA suppresses the parasitic load in mϕ (kDNA expression) and promotes the Th-1 type immune response (IL-12, iNOS). However, no significant change in kDNA expressions was observed when L. donovani promastigotes were treated with LA. Intrigued by this observation, we explored mechanism(s) by which LA promoted the protective type immune response in infected mϕ. Interestingly, LA decreased the release of L. donovani derived extracellular vesicle later characterized as microvesicles. Moreover, these microvesicles were suppressive concerning their bias toward the Th-2 type of immune responses (IL-10, Arginase) in mϕ. We suggest that LA plays a protective role in the immune response against L. donovani infection by inhibiting the release to Leishmania derived microvesicles and thus promoting Th-1 type immune response in mϕ.

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Section: Discussionmentioning

confidence: 99%

“…ESCO CelSafe, Esco Micro Pvt. Ltd., Singapore) at MNNIT Allahabad (Saini et al, 2019 ). For experiments, 2 × 10 6 mϕ cells were counted with the help of the Neubauer chamber and seeded in the six well plates.…”

Section: Methodsmentioning

confidence: 99%

Linoleic Acid Inhibits the Release of Leishmania donovani Derived Microvesicles and Decreases Its Survival in Macrophages

Saini¹,

Kumar²

2020

Front. Cell. Infect. Microbiol.

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“…(Ptgds2, Ptges) was also evident at this timepoint. PTGES is an arachidonic acid metabolite produced by immune cells, that has been recognized to support establishment of infection in macrophages, and hamsters infected by L. donovani (23).…”

Section: Discussionmentioning

confidence: 99%

Integrated multivariate analysis of transcriptomic data reveals immunological mechanisms in mice afterLeishmania infantuminfection

Palacios

Diaz-Solano²,

Valladares³

et al. 2020

Preprint

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Transcriptional analysis of complex biological scenarios has been extensively used, even though sometimes results may prove imprecise or difficult-to interpret due to an overwhelming amount of information. In this study, a large-scale Real-time qPCR experiment was coupled to multivariate statistical analysis to describe the main immunological events underlying the early L. infantum infection in livers of BALB/c mice. High-throughput qPCR was used to evaluate the expression of 223 genes related to immunometabolism 1-, 3-, 5- and 10-days post infection. This integrative analysis showed strikingly different gene signatures at 1- and 10-days post infection, revealing progression of infection in the experimental model based on the upregulation of particular immunological response patterns and mediators. This approach addresses the challenge of integrating large collections of transcriptional data for the identification of candidate biomarkers in experimental models.

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“…On the other hand, LTB4 increases vascular permeability, enhances ROS generation and promotes NK cell activity (16). LTB4 enhances pro-inflammatory mediators (TNF, IL-1, IL-6, IL-2, IL-12, IFN-γ, and iNOS) and loss of 5-lipoxygenase (LTB4 final product) leads to decreased phagocytosis of by mφ and neutrophils (24,25).…”

Section: Leishmania Infection and ω-6 Polyunsaturated Fatty Acids (Pufa)mentioning

confidence: 99%

Linoleic Acid—A Feasible Preventive Approach for Visceral Leishmaniasis

Saini

Kumar

2021

Front. Nutr.

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Parasitic load determination by differential expressions of 5-lipoxygenase and PGE2 synthases in visceral leishmaniasis

Cited by 11 publications

References 34 publications

Linoleic Acid Inhibits the Release of Leishmania donovani Derived Microvesicles and Decreases Its Survival in Macrophages

Linoleic Acid Inhibits the Release of Leishmania donovani Derived Microvesicles and Decreases Its Survival in Macrophages

Integrated multivariate analysis of transcriptomic data reveals immunological mechanisms in mice afterLeishmania infantuminfection

Linoleic Acid—A Feasible Preventive Approach for Visceral Leishmaniasis

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