Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods

Gariépy, Tara D.; Kuhlmann, Ulrich; Gillott, Cedric; Erlandson, Martin A.

doi:10.1111/j.1439-0418.2007.01145.x

Cited by 174 publications

(151 citation statements)

References 103 publications

(257 reference statements)

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“…PCR-based techniques are also being used in an insect pest management context to identify insect pests and insect biotypes, to understand population structure, tritrophic interactions and insect-plant relationships (Caterino et al, 2000;Heckel, 2003;Gariepy et al, 2007). For this purpose the ribosomal and mitochondrial regions have been proven to be highly informative.…”

Section: Molecular Tools For Detection and Identification Of Plant Pamentioning

confidence: 99%

“…For this purpose the ribosomal and mitochondrial regions have been proven to be highly informative. In particular the cytochrome c oxidase I and II (COI and COII) and the 16S and 12S subunits of mitochondrial DNA as well as the internal transcribed spacer regions (ITS1 and ITS2), the 18S and the 28S subunits of the rDNA are used (Gariepy et al, 2007).…”

Section: Molecular Tools For Detection and Identification Of Plant Pamentioning

confidence: 99%

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DNA Sequencing and Crop Protection

Tedeschi¹

2012

DNA Sequencing - Methods and Applications

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Section: Molecular Tools For Detection and Identification Of Plant Pamentioning

confidence: 99%

Section: Molecular Tools For Detection and Identification Of Plant Pamentioning

confidence: 99%

DNA Sequencing and Crop Protection

Tedeschi¹

2012

DNA Sequencing - Methods and Applications

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“…(KOX et al 2005;BLACKET et al 2015). Among these techniques, the DNA barcoding has enormous potential for identification of cryptic species (GARIEPY et al, 2007;JENKINS;CHAPMAN;MICALLEF, 2012;HEBERT et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Hebert et al (2003) proposed the use of a cox I fragment as a DNA barcode, useful for identifying various metazoan groups. DNA barcoding has been used to identify different species of insect pests, endemic and non-endemic, and their natural enemies (GARIEPY et al, 2007). It is a reliable, quick technique that can be applied at any developmental stage of all insects (JENKINS; CHAPMAN;MICALLEF, 2012;DE SOUZA FREITAS et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

MOLECULAR IDENTIFICATION OF Liriomyza sp. IN THE NORTHEAST AND SOUTHEAST REGIONS OF BRAZIL

et al. 2017

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-In Brazil, species of the genus Liriomyza are widely distributed and have economic importance as they cause damage to at least 14 plant families, especially Solanaceae, Cucurbitaceae, Asteraceae, and Fabaceae. Studies suggest existence of a species complex within this genus, based on the presence of morphological similarities among the species Liriomyza trifolii (Burgess), L. sativae Blanchard and L. huidobrensis (Blanchard). The present study aimed to use DNA barcoding to establish new distribution records of L. sativae in distinct regions in Brazil, determine intra-and inter-population genetic diversity, and reconstruct the phylogeny of Liriomyza species using the DNA barcode sequences. Identity values were between 97% and 99%, confirming that all the examined Brazilian populations belonged to the species L. sativae. Phylogenetic analyses indicated the presence of a single clade of L. sativae, composed of seven populations. Intra-population analysis on individuals of these populations indicated low levels of nucleotide and haplotype diversity. The haplotype network indicated presence of only 14 haplotypes distributed among the Brazilian populations. The genetic similarities shared by the Brazilian populations of L. sativae suggest that these populations are closely related. Genetic patterns observed among populations of L. sativae might be associated with bottleneck events or founder effect during establishment of this leafminer in Brazil.Keywords: Cryptic species. DNA barcoding. Population genetics. IDENTIFICAÇÃO MOLECULAR DE Liriomyza sp. NAS REGIÕES NORDESTE E SUDESTE DO BRASILRESUMO -No Brasil, as espécies do gênero Liriomyza têm importância econômica e são amplamente distribuídos no país, causando danos a pelo menos 14 famílias de plantas, especialmente Solanaceae, Cucurbitaceae, Asteraceae e Fabaceae. Estudos sugerem a existência de um complexo de espécies dentro deste gênero com base na presença de semelhança morfológica nas espécies Liriomyza trifolii (Burgess), L. sativae (Blanchard) e L. huidobrensis (Blanchard). Este estudo teve como objetivo empregar o DNA Barcode em novas áreas para estabelecer novos registros de Liriomyza sativae no Brasil e também a determinação da diversidade genética intra e interpopulacional, e reconstruir a filogenia das espécies Liriomyza utilizando sequências do DNA barcode. Os valores de identidade foram entre 97% a 99%, confirmando que as todas as populações brasileiras avaliadas pertencem à espécie L. sativae. A análise filogenética indicou a presença de um único clado de L. sativae composto pelas sete populações. A análise intrapopulacional indicou níveis baixos de diversidade nucleotidica e haplótipica de indivíduos dessas populações. A rede de haplótipos indicou a presença de apenas 14 haplótipos distribuídos entre populações brasileiras. As semelhanças genéticas compartilhadas pelas populações brasileiras de L. sativae sugere que essas populações estão intimamente relacionados. Os padrões genéticos observados em populações de L. sativae pode estar associad...

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“…DNA based techniques provide advantages over conventional rearing and dissection methods (Gariepy et al, 2007;Greenstone et al, 2011). Advantages include fewer lag times, avoidance of data loss due to emergence failures (parasitoid / hyperparasitoid mortality), and the identification of immature stages which are generally difficult to distinguish based on morphology (Walton et al, 1990;Gariepy et al, 2008).…”

Section: Cover Management As Conservation Biological Control Of Aphidmentioning

confidence: 99%

Integrated Pest Management of Aphis spiraecola (Hemiptera: Aphididae) in clementines: enhancing its biological control

Marco¹

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A ma mare "la entrada del neolítico es la del abuso y la del sojuzgamiento, y en ella seguimos, inadaptados" Félix Rodríguez de la FuenteAgraïments Agraixc als meus directors Alberto Urbaneja i Alejandro Tena tota la feina que han fet i que m'han empentat a fer per aconseguir este primer escaló.Gracies Alberto per la teua confiança i dedicació des de abans del primer dia de doctorat, després de cinc anys i una tesi, ara ja podem ser amics.Gracies Alejandro per estar pendent cada dia, amb eixa dedicació e il•lusió.He disfrutat molt dels nostres debats científics. Ara ja soc mig enginyer?També he de agrair a Josep Jaques la seua col•laboració en gran part de la tesi. Gracies Josep per aportar de la teua clarividència. Es molt fàcil aprendre amb tres mostres com estos.Vull agrair infinitament a Pablo Bru tot el temps dedicat i tota la faena que hem compartit al camp. Sense la seua ajuda gran part de la tesis no haguera sigut possible. I per suposat a tots aquells que també han compartit hores de sol i calor, fang, pluja i fred o inclús rossejades de aplicacions quimiques. Vull agrair de una manera especial a dos persones que foren molt importants en dos moments claus de la meua carrera com Entomòleg. Sense la seua espenta en eixe instant, no estaria ací. Gracies Manel Porcar i Paco Beitia.I també a totes aquelles persones que durant mes de deu anys en convertiren de Biòleg a Entomòleg. Gracies a Lara Navarro, Teresa Oltra, Olaf Strauss, Axel Gruppe, Rosa Vercher i Ferran Garcia Marí, Tatiana Pina, Alfonso Hermoso de Mendoza, Maria Jesus Verdú, Jose Manuel Michelena, Paul Rugman-Jones i un llarg etc.Agrair a tots els meus amics i a totes aquelles persones especials que han compartit en mi el temps mentre esta tesi prenia forma. Sense ells, haguera sigut un viatge mes pesat.Especialment a Marta, en tu tot es molt més fàcil, ara et toca a tu.Nomes hem falta agrair a ma mare i mon pare tot el que han fet per a que el meu somni continués endavant, i sobre tot per els valors que m'ensenyaren de humilitat i perseverança. ResumAphis spiraecola Patch. (Hemiptera: Aphididae) és una de les plagues clau en el cultiu de clementins de la conca Mediterrània. En primavera, este àfid colonitza les brotacions tendres dels clementins i provoca importants pèrdues econòmiques tots els anys. Actualment la gestió integrada d"A. spiraecola en clementins està basada en el control químic ja que es desconeix prou sobre el control biològic d"A. spiraecola en cítrics i els esforços realitzats fins ara s"han centrat en el us i conservació de parasitoids encara que es desconeix les causes de la seua baixa eficàcia. Per altra banda, es coneix molt bé el conjunt de depredadors d"A. spiraecola però el seu impacte sobre les poblacions de l"àfid no s"han documentat. Tenint en compte estos antecedents, els objectius d"esta tesis foren: i) desentrampar les raons per les quals els parasitoids no són efectius; ii) determinar quan i com els depredadors poden controlar les poblacions d"A. spiraecola; i finalment iii) determinar si una coberta de poàcies po...

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Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods

Cited by 174 publications

References 103 publications

DNA Sequencing and Crop Protection

DNA Sequencing and Crop Protection

MOLECULAR IDENTIFICATION OF Liriomyza sp. IN THE NORTHEAST AND SOUTHEAST REGIONS OF BRAZIL

Integrated Pest Management of Aphis spiraecola (Hemiptera: Aphididae) in clementines: enhancing its biological control

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