Parasitological diagnosis in fecal samples in the Laboratory of Clinical
                        Analyzes: comparison of techniques and cost of implantation

Azevedo, Eduarda Peixoto; Almeida, Elida Mateus de; Matos, Juliana da Silva; Ramos, Andreza Rodrigues; Siqueira, Mayara Perlingeiro de; Fonseca, Ana Beatriz Monteiro; Barbosa, Alynne da Silva; Bastos, Otílio Machado Pereira; Uchôa, Cláudia Maria Antunes

doi:10.21877/2448-3877.201700586

Cited by 3 publications

(5 citation statements)

References 15 publications

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“…It should be noted that only one slide was made per sample, in order to simulate a clinical analyzes laboratory routine with a large number of coproparasitological exams. According to Azevedo et al (2017), the time, cost and complexity of coproparasitological examination methods are important factors in the choice of methods applied in routine laboratory examination.…”

Section: Methodsmentioning

confidence: 99%

Willis Method Modification With a View to Improving Routine Coproparasitological Diagnosis

Coêlho

Faria²,

Maciel³

et al. 2019

Rev Patol Trop

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Intestinal parasites still cause high morbidity and mortality, due to poor hygiene and sanitaryconditions, and therefore indiscriminate treatment has been routine practice advocated by thePublic Health staff. Although there is a consensus regarding the need to diagnose such diseases,this is not performed with the necessary care, due to great demand and the lack of a wide rangedand highly sensitive technique. In this sense, most clinical laboratories use routine methods forfecal examination such as the Lutz sedimentation or modified Ritchie methods, which are completeand easy to execute, but do not have adequate sensitivity to detect low density eggs and protozoancysts, especially when there is a predominance of low parasite burdens. In contrast, there aremethods that are based on the flotation of low density developmental forms, namely, the Willismethod (NaCl flotation d=1.120), which is rapid, easy to perform and allows high density eggflotation but with low sensitivity for protozoan cysts; and the Faust method, which is based oncentrifugal flotation of developmental forms in a 33% ZnSO4 (d=1.200) solution, but with thedisadvantage of being lengthy and requiring a centrifuge. In this study, we verified the applicabilityof introducing an alteration in the Willis method, which consisted in the substitution of NaCl byZnSO4 in order to combine the advantages of this method with the Faust method. 208 samples wereassessed by the Willis and Ritchie methods and by the proposed method (modified Willis). Thelatter proved superior to the other two (ρ <0,0001 – X2) regarding the detection of protozoan cysts,but similar to the Ritchie method in regard to other diagnosed parasites, therefore demonstratingthe high potential for the introduction of this modified method in the routine of fecal diagnosis.

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Section: Methodsmentioning

confidence: 99%

Willis Method Modification With a View to Improving Routine Coproparasitological Diagnosis

Coêlho

Faria²,

Maciel³

et al. 2019

Rev Patol Trop

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show abstract

“…Os resultados obtidos, foram apresentados de forma descritiva, a concordância de positividade/negatividade por amostra entre as técnicas foi analisada pelo teste de McNemar com intervalo de confiança de 5% e a concordância geral por meio do índice de concordância (Kappa) (Azevedo et al, 2017).…”

Section: Metodologiaunclassified

“…O diagnóstico clínico é impreciso, pois baseia-se em manifestações clínicas, que, nesse caso, podem variar desde quadros assintomáticos a outros de sintomatologias inespecíficas, tais como diarreia, náuseas, desconforto abdominal, dentre outros. Portanto, o diagnóstico laboratorial desempenha um papel importante no diagnóstico das infecções/doenças parasitárias, sendo a chave para a seleção da conduta terapêutica (Azevedo et al, 2017).…”

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Perfil de desempenho de técnicas parasitológicas na detecção de helmintíases em área endêmica do nordeste do Brasil

Santos

Vitória

Siqueira

et al. 2022

RSD

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O estudo visa comparar a eficácia e a sensibilidade de três técnicas parasitológicas no diagnóstico das helmintíases em uma área endêmica no nordeste do Brasil. Trata-se de um estudo epidemiológico, descritivo e comparativo, o mesmo foi realizado no município de Maruim, que fica aproximadamente a 30 km de da capital do estado de Sergipe, a cidade de Aracaju. Foram distribuídos kits e coletadas 3 amostras diárias entre os dias 17 a 20 de setembro de 2018, posteriormente as amostras foram transportadas até o laboratório de Entomologia e Parasitologia Tropical da Universidade Federal de Sergipe para realizar análise macroscópica, aplicando o método de Kato-Katz, Hoffman, Pons e Janer, e o método de Ritchie. Foi encontrado uma maior prevalência de casos positivos para Schistosoma mansoni (48,12%), ancilostomídeos (4,38%) e Hymenolepis nana (2,50%) quando analisado sob a técnica de Kato -Katz, os helmintos Enterobius vermicularis (5,62%), Trichuris trichiura (36,25%) e Strongyloides stercoralis (5,00%) mostrou-se mais encontrado quando utilizado a técnica de Ritchie. As técnicas se mostraram bastante eficientes na identificação dos parasitas, porém nem todos os parasitos foram identificados simultaneamente pelas técnicas. Com base nesta informação, conclui-se que uma técnica pode complementar a outra no diagnóstico parasitário aumentando assim sua sensibilidade.

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“…On the other hand, Sanchez [7] using direct examination for diagnosing B. coli in human stool samples, and Barbosa et al [8] compared five coprological techniques for the diagnosis of B. coli cysts in fecal samples from pigs and non-human primates. Azevedo et al [9] used four parasitological techniques to study intestinal parasites in fecal samples from people from Brazil.…”

Section: Introductionmentioning

confidence: 99%

“…Azevedo et al . [ 9 ] used four parasitological techniques to study intestinal parasites in fecal samples from people from Brazil.…”

Section: Introductionmentioning

confidence: 99%

Comparison between five coprological methods for the diagnosis of Balantidium coli cysts in fecal samples from pigs

Pinilla

Florez

2021

Vet World

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Background and Aim: Balantidium coli is a protozoan that can infect humans and non-human primates, being the domestic pigs the animals most affected by this parasite. This study aimed to compare the performance of five coprological methods for diagnosis of Balantidium coli cysts in fecal samples from pigs. Materials and Methods: From September to December 2019, 558 fecal samples were collected from the rectum of backyard pigs in the Bucaramanga metropolitan area, Colombia. The average age of the sampled animals was 3-4 months. Fecal samples collected were tested using the following coprological techniques: Direct examination with Lugol's iodine solution, buffered saline, centrifugation/flotation and McMaster techniques, and Ziehl–Neelsen method. Results: The results indicate that B. coli cysts were diagnosed most frequently through direct examination with Lugol's iodine solution (52.7% of the samples) followed by direct examination with a buffered saline solution (37.6%). Moderate concordance (k=0.41; p<0.05) was determined between direct method with Lugol's iodine and buffered saline solution, McMaster and buffered saline (k=0.35; p<0.05), and centrifugation/flotation and buffered saline (k=0.28; p<0.05) showed a fair degree of concordance. The rest of the comparisons were classified as poor. The flotation techniques (centrifugation/ flotation and McMaster) did not show good recovery of cysts. Conclusion: It is concluded that the most efficient method for diagnosing this parasitosis was to an association between direct examination with Lugol's iodine solution and buffered saline.

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Parasitological diagnosis in fecal samples in the Laboratory of Clinical Analyzes: comparison of techniques and cost of implantation

Cited by 3 publications

References 15 publications

Willis Method Modification With a View to Improving Routine Coproparasitological Diagnosis

Willis Method Modification With a View to Improving Routine Coproparasitological Diagnosis

Perfil de desempenho de técnicas parasitológicas na detecção de helmintíases em área endêmica do nordeste do Brasil

Comparison between five coprological methods for the diagnosis of Balantidium coli cysts in fecal samples from pigs

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