Parathyroid Cell Differentiation from Progenitor Cells and Stem Cells: Development, Molecular Mechanism, Function, and Tissue Engineering

Şenkal, Selinay; Doğan, Ayşegül

doi:10.1007/5584_2021_694

Cited by 2 publications

(3 citation statements)

References 56 publications

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“…Hypoparathyroidism is an endocrine disorder characterized by low serum calcium levels due to low or absent PTH secretion 2 . The most common cause of hypoparathyroidism is injuries or accidental removal of parathyroid glands during thyroid surgery, which comprises around 75% of all cases 3–5 . Conventional therapy including calcium supplements, vitamin D supplements and thiazide diuretics only increases the patient's serum calcium concentration but does little for the complications of hypoparathyroidism 6,7 .…”

Section: Introductionmentioning

confidence: 99%

“… 2 The most common cause of hypoparathyroidism is injuries or accidental removal of parathyroid glands during thyroid surgery, which comprises around 75% of all cases. 3 , 4 , 5 Conventional therapy including calcium supplements, vitamin D supplements and thiazide diuretics only increases the patient's serum calcium concentration but does little for the complications of hypoparathyroidism. 6 , 7 The recombinant human parathyroid hormone (rhPTH), as a treatment, was reported to cause osteosarcoma.…”

Section: Introductionmentioning

confidence: 99%

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Directed differentiation of human embryonic stem cells into parathyroid cells and establishment of parathyroid organoids

Wang,

Du,

Cui

et al. 2024

Cell Proliferation

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Differentiation of human embryonic stem cells (hESCs) into human embryonic stem cells‐derived parathyroid‐like cells (hESC‐PT) has clinical significance in providing new therapies for congenital and acquired parathyroid insufficiency conditions. However, a highly reproducible, well‐documented method for parathyroid differentiation remains unavailable. By imitating the natural process of parathyroid embryonic development, we proposed a new hypothesis about the in vitro differentiation of parathyroid‐like cells. Transcriptome, differentiation marker protein detection and parathyroid hormone (PTH) secretion assays were performed after the completion of differentiation. To optimize the differentiation protocol and further improve the differentiation rate, we designed glial cells missing transcription factor 2 (GCM2) overexpression lentivirus transfection assays and constructed hESCs‐derived parathyroid organoids. The new protocol enabled hESCs to differentiate into hESC‐PT. HESC‐PT cells expressed PTH, GCM2 and CaSR proteins, low extracellular calcium culture could stimulate hESC‐PT cells to secrete PTH. hESC‐PT cells overexpressing GCM2 protein secreted PTH earlier than their counterpart hESC‐PT cells. Compared with the two‐dimensional cell culture environment, hESCs‐derived parathyroid organoids secreted more PTH. Both GCM2 lentiviral transfection and three‐dimensional cultures could make hESC‐PT cells functionally close to human parathyroid cells. Our study demonstrated that hESCs could differentiate into hESC‐PT in vitro, which paves the road for applying the technology to treat hypoparathyroidism and introduces new approaches in the field of regenerative medicine.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Directed differentiation of human embryonic stem cells into parathyroid cells and establishment of parathyroid organoids

Wang,

Du,

Cui

et al. 2024

Cell Proliferation

View full text Add to dashboard Cite

show abstract

“…The physiological maintenance of calcium levels is mainly regulated by the gastrointestinal tract, bones and kidneys. Parathormone (PTH) is released by the parathyroid gland and regulates calcium within narrow limits by increasing calcium absorption in the intestine, bone-calcium mobilization and calcium reabsorption by the kidneys [ 3 ]. Calcium ions are considered to be the principal physiological regulators of PTH secretion via CaSRs [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Applications of Metabolomics in Calcium Metabolism Disorders in Humans

Podgórska

Wielogórska

Godzień

et al. 2022

IJMS

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The pathogenesis of the disorders of calcium metabolism is not fully understood. This review discusses the studies in which metabolomics was applied in this area. Indeed, metabolomics could play an essential role in discovering biomarkers and elucidating pathological mechanisms. Despite the limited bibliography, the present review highlights the potential of metabolomics in identifying the biomarkers of some of the most common endocrine disorders, such as primary hyperparathyroidism (PHPT), secondary hyperparathyroidism (SHPT), calcium deficiency, osteoporosis and vitamin D supplementation. Metabolites related to above-mentioned diseorders were grouped into specific classes and mapped into metabolic pathways. Furthermore, disturbed metabolic pathways can open up new directions for the in-depth exploration of the basic mechanisms of these diseases at the molecular level.

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Parathyroid Cell Differentiation from Progenitor Cells and Stem Cells: Development, Molecular Mechanism, Function, and Tissue Engineering

Cited by 2 publications

References 56 publications

Directed differentiation of human embryonic stem cells into parathyroid cells and establishment of parathyroid organoids

Directed differentiation of human embryonic stem cells into parathyroid cells and establishment of parathyroid organoids

Applications of Metabolomics in Calcium Metabolism Disorders in Humans

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