Parathyroid Hormone, Carbonic Anhydrase and Calcium Homeostasis

Dietsch, Peter

doi:10.1007/978-3-642-73346-8_24

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…It has been demonstrated that PTH treatment of osteoclasts activates carbonic anhydrase [Silverton et al, 1987;Dietsch, 1987] and that this activation appears to be due to phosphorylation through protein kinase A [Dietsch, 1987]. The activation of carbonic anhydrase results in an increased supply of hydrogen ions, which would then be translocated across the plasma membrane by the proton pump [reviewed by Gay, 1992;Delaissé and Vaes, 1992].…”

Section: Discussionmentioning

confidence: 98%

Parathyroid hormone uses both adenylate cyclase and protein kinase C to regulate acid production in osteoclasts

May

1997

J. Cell. Biochem.

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Osteoclasts, isolated from the endosteum of 2.5- to 3-week-old chickens, were treated with acridine orange, a hydrogen ion concentration-sensitive fluorescent dye, in order to monitor changes in acid production. The adenylate cyclase inhibitor, alloxan, blocked parathyroid hormone (PTH)-stimulated acid production. Dibutyryl cyclic adenosine monophosphate, a membrane-permeant form of cyclic adenosine monophosphate, mimicked the PTH effect. Bisindolylmaleimide, a specific inhibitor of protein kinase C (PKC), blocked the initial stimulation (15, 30, and 60 min) of acid production by PTH but had no effect on long-term stimulation (120 min). Confocal microscopy of osteoclasts stained with fluorescein-conjugated bisindolylmateimide revealed a shift in location of PKC from the cytoplasm to the plasma membrane region after treatment with parathyroid hormone. The results of these studies support the hypothesis that PTH regulation of acid production in osteoclasts involves both adenylate cyclase and PKC as effectors.

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Section: Discussionmentioning

confidence: 98%

Parathyroid hormone uses both adenylate cyclase and protein kinase C to regulate acid production in osteoclasts

May

1997

J. Cell. Biochem.

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“…In an earlier study, PTH was found to stimulate CAMP levels in isolated osteoclasts (It0 et al, 1985). Carbonic anhydrase has been identified as an effector molecule for the PTHcAMP signaling pathway (Dietsch, 1987;Silverton et al, 1987). It is possible that these actions of PTH occur as a result of direct activation on osteoclasts.…”

Section: Insights Into Osteoclast Acidification Usingmentioning

confidence: 99%

Role of microscopy in elucidating the mechanism and regulation of the osteoclast resorptive apparatus

Gay¹

1996

Microsc. Res. Tech.

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Specific binding of parathyroid hormone to living osteoclasts

Agarwala

Gay

1992

Journal of Bone and Mineral Research

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We show that osteoclasts bind parathyroid hormone (PTH) in a manner that displays the properties of receptor-dependent hormone binding, that is, saturability, time dependence, temperature dependence, and hormone specificity. Osteoclasts were isolated from the endosteum of 2 to 3 week chick tibiae and maintained in culture for 4-6 days. Bovine PTH-(1-84) was biotinylated with N-hydroxysuccinimidobiotin. Biotinyl-PTH (btPTH, 10(-5)-10(-11) M) was added to the cultured osteoclasts for 2-20 minutes. After rinsing away unbound btPTH, fluorescein isothiocyanate-labeled avidin (FITC-avidin) at a concentration of 66 micrograms/ml was applied. Receptor binding characteristics were assessed: (1) saturation occurred at around 10(-6) M btPTH; (2) competition of excess unlabeled PTH was found, namely, a 10-fold excess abolished fluorescence; (3) specificity was shown by adding other polypeptide hormones (insulin, glucagon, and calcitonin) in 10- to 100-fold excess--no effect on PTH binding was observed; and (4) affinity of btPTH for its binding site was indicated by half-maximal binding approximately equal to 10(-7) M for both osteoclasts and osteoblasts. Biotin (10(-5) M) or FITC-avidin (66 micrograms/ml) alone did not cause fluorescence. The time course of btPTH on the cell exterior was short: at 2 and 5 minutes dots of fluorescence were randomly dispersed over the cell surface, by 10 minutes most of the fluorescence was clustered in one region of the membrane, and by 20 minutes most of the hormone was no longer present on the surface of the cells.(ABSTRACT TRUNCATED AT 250 WORDS)

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Parathyroid Hormone, Carbonic Anhydrase and Calcium Homeostasis

Cited by 5 publications

References 16 publications

Parathyroid hormone uses both adenylate cyclase and protein kinase C to regulate acid production in osteoclasts

Parathyroid hormone uses both adenylate cyclase and protein kinase C to regulate acid production in osteoclasts

Role of microscopy in elucidating the mechanism and regulation of the osteoclast resorptive apparatus

Specific binding of parathyroid hormone to living osteoclasts

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