Parathyroid Hormone (PTH) Assays and Applications to Bone Disease: Overview on Methodology

Arya, Ashutosh Kumar; Sachdeva, Naresh

doi:10.1007/978-94-007-7745-3_6-1

Cited by 3 publications

(4 citation statements)

References 77 publications

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“…The identification and quantification of circulating PTH fragments has proved to be a barrier to the development of reliable PTH immunoassays because it is recognized that some PTH fragments could crossreact with PTH immunoassay-based platforms, resulting in high assay variability. Three generations of PTH immunoassays have been developed over the past 60 years, each targeting a different portion of the PTH 1–84 molecule 54 …”

Section: Current Methodologies For Pth Measurementmentioning

confidence: 99%

“…53 In the aforementioned studies, it should be noted that the investigators did not provide data on directly measured oxPTH. Nonetheless, by using the mass spectrometry-based assay, we have found that all species of oxPTH 54 First-generation PTH competitive RIA used a single polyclonal antibody directed toward the amino-terminal, midregion, or carboxy-terminal portion of PTH 1-84. Because these antibodies detect both PTH 1-84 and various PTH fragments, assays do not reflect levels of biologically active PTH 1-84.…”

Section: Use Of Lc-hrms To Confirm the Existence Of Post-translationa...mentioning

confidence: 99%

“…In these immunoassays, one "capture" antibody directed toward the carboxy-terminal portion (e.g., amino acid positions 39-84) captures PTH 1-84 or PTH fragments from serum, and a second ("reporter") antibody directed toward the amino-terminal portion of PTH 1-84 (e.g., amino acid positions 12-24 or 26-32) is used to quantitate the amount of captured peptide. 54 Because two antibodies are required for detection in the assay procedure, only relatively long PTH forms, containing both targeted epitopes, are recognized by PTH sandwich immunoassays. Small PTH fragments lacking one or both epitopes are not detected.…”

Section: Use Of Lc-hrms To Confirm the Existence Of Post-translationa...mentioning

confidence: 99%

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High-Resolution Mass Spectrometry for the Measurement of PTH and PTH Fragments: Insights into PTH Physiology and Bioactivity

Ulmer

Kritmetapak

Singh

et al. 2022

JASN

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Full-length parathyroid hormone (PTH 1-84) is crucial for the regulation of calcium and phosphate homeostasis and bone remodeling. PTH 1-84 is metabolized into various PTH fragments, which are measured with varying levels of efficiency by PTH immunoassays. These PTH fragments, which increase in serum as CKD progresses, could potentially modulate the effects of PTH 1-84 and contribute to CKD-associated bone disorders. To obtain a true biological representation of total PTH bioactivity, it necessary to measure not only PTH 1-84, but also PTH fragments that are present in circulation. Traditional second-generation PTH immunoassays collectively measure PTH 1-84, PTH fragments, and post-translationally modified PTH 1-84, making it difficult to accurately predict the character of underlying renal osteodystrophy. This review highlights current advances in methods available for PTH measurement and the clinical relevance of PTH fragments in CKD. We emphasize the usefulness of mass spectrometry as a potential reference method for PTH measurement.

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Section: Current Methodologies For Pth Measurementmentioning

confidence: 99%

Section: Use Of Lc-hrms To Confirm the Existence Of Post-translationa...mentioning

confidence: 99%

Section: Use Of Lc-hrms To Confirm the Existence Of Post-translationa...mentioning

confidence: 99%

See 1 more Smart Citation

High-Resolution Mass Spectrometry for the Measurement of PTH and PTH Fragments: Insights into PTH Physiology and Bioactivity

Ulmer

Kritmetapak

Singh

et al. 2022

JASN

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“…Elecsys1010 was utilized to assess PTH by immunological testing utilizing the electrochemiluminescence immunoassay (ECLIA) method described by Arya and Sachdeva [23]. It is based on a mechanism in which highly reactive species are created from stable precursors at the surface of an electrode.…”

Section: Estimation Of Serum Parathyroid Hormone (Pth) Levelsmentioning

confidence: 99%

Association of genetic variants located at the Adenylate Cyclase 5 (ADCY5) and Parathyroid Hormone 1 Receptor (PTH1R) genes with type 2 Diabetes Mellitus in Bangladeshi population

Nawar

Alam

et al. 2023

Preprint

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Background Genome-Wide Association Studies have identified genetic polymorphisms located at the Adenylate Cyclase 5 (ADCY5) and Parathyroid Hormone 1 Receptor (PTH1R) genes on chromosome 3 as susceptibility loci for type 2 diabetes mellitus (T2DM) in different population. The present study investigates the association of these two susceptibility loci (rs11708067 at the ADCY5 and rs11926707 at the PTH1R gene) with T2DM in Bangladeshi population. Furthermore, the association of circulating levels of vitamin D, Calcium (Ca), phosphate, and parathyroid hormone (PTH) with T2DM and the different genotypes of these SNPs were also studied.Methods and Materials A total of 415 individuals (211 T2DM patients and 204 healthy controls) were enrolled in the current study. The rs11708067 and rs11926707 were genotyped using the Taqman SNP Genotyping Assays. Univariate logistic regression analyses were performed for parameter associations with T2DM.Results In a sex-stratified analysis, the variant allele of the ADCY5 SNP (rs11708067) was associated to T2DM in males (odds ratio = 2.5, p-value = 0.02) but PTH1R SNP (rs11926707) did not show any significant correlation with T2DM (odds ratio 1.0 in both sexes, p-value > 0.05). In the correlation study, phosphate was shown to be significantly linked with Ca (p-value < 0.001) and PTH (p-value < 0.05) in both T2DM and healthy individuals. Lastly, neither SNP revealed any link between vitamin D, calcium, nor phosphate levels, with the exception of PTH.Conclusion Although ADCY5 has been identified as a T2DM susceptible locus in the Bangladeshi male population, the same cannot be said for the PTH1R locus in either males or females.

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Parathyroid Hormone (PTH) and the Relationship Between PTH and Bone Health: Structure, Physiology, Actions, and Ethnicity

Gaffney‐Stomberg¹,

MacArthur²,

McClung³

2016

Biomarkers in Bone Disease

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Parathyroid Hormone (PTH) Assays and Applications to Bone Disease: Overview on Methodology

Cited by 3 publications

References 77 publications

High-Resolution Mass Spectrometry for the Measurement of PTH and PTH Fragments: Insights into PTH Physiology and Bioactivity

High-Resolution Mass Spectrometry for the Measurement of PTH and PTH Fragments: Insights into PTH Physiology and Bioactivity

Association of genetic variants located at the Adenylate Cyclase 5 (ADCY5) and Parathyroid Hormone 1 Receptor (PTH1R) genes with type 2 Diabetes Mellitus in Bangladeshi population

Parathyroid Hormone (PTH) and the Relationship Between PTH and Bone Health: Structure, Physiology, Actions, and Ethnicity

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