Paratuberculose em bovinos de corte na região Sul do Rio Grande do Sul

Fiss, Letícia; Santos, Bianca Lemos dos; Albuquerque, Pedro Paulo Feitosa de; Mota, Rinaldo Aparecido; Marcolongo-Pereira, Clairton; Adrien, María de Lourdes; Soares, Mauro Pereira; Schild, Ana Lúcia

doi:10.1590/s0100-736x2015000500008

Cited by 9 publications

(8 citation statements)

References 23 publications

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“…Clinical signs such as progressive weight loss, dehydration, homogeneous and non-hemorrhagic feces, without mucus and with bubbles gas were also found by other authors in cattle with paratuberculosis (Fiss et al, 2015;Driemeier et al, 1999).…”

Section: Discussionsupporting

confidence: 75%

Clinical bovine paratuberculosis - first report in the state of Pará

Barbosa,

Serruya,

Lins

et al. 2023

Arq. Bras. Med. Vet. Zootec.

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The objective of this work was to describe the clinical, pathological, and serological aspects of paratuberculosis in a bullock on dairy and beef farm in the municipality of Inhangapí, state of Pará. The animal was raised extensively and presented progressive weight loss, pale mucous membranes, and chronic diarrhea. A blood sample was collected for serology by the indirect ELISA technique. Due to the unfavorable prognosis, the animal was euthanized and necropsied. At necropsy, there was thickened and wrinkled intestinal mucosa, enlarged mesenteric lymph nodes and liquid intestinal contents with a brown color. Organ fragments were fixed in 10% buffered formaldehyde, embedded in paraffin wax, cut, and stained by hematoxylin and eosin and by Ziehl-Neelsen techniques. Histopathology showed intense granulomatous infiltration with Langhans giant cells in the lamina propria and submucosa of the small intestine, Peyer patches and mesenteric lymph nodes. Ziehl-Neelsen staining revealed many intralesional acid-fast bacilli. Antibody searching against Mycobacterium avium (subsp.) paratuberculosis was positive. The diagnosis of paratuberculosis was based on epidemiological, clinical, pathological, and serological data. This is the first report of clinical paratuberculosis, confirmed by pathology and serology, in a bullock in the state of Pará.

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Section: Discussionsupporting

confidence: 75%

Clinical bovine paratuberculosis - first report in the state of Pará

Barbosa,

Serruya,

Lins

et al. 2023

Arq. Bras. Med. Vet. Zootec.

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“…The mesoregions with only negative samples for both ELISA and PCR also had a smaller sample size since the number of dairy cows in those regions was smaller, highlighting the need for further studies in these regions. Indeed, cases of clinical paratuberculosis have been reported in the southeastern mesoregion (Fiss et al 2015) and the metropolitan region (Driemeier et al 1999, Gomes et al 2002, while our study found no positive results in the southeastern region and did not include any samples from the metropolitan region.…”

Section: Discussioncontrasting

confidence: 65%

“…To the best of our knowledge, only one robust and current study has informed the detection of antibodies anti MAP in cattle from the northeast region, more precisely in the state of Pernambuco (Yamasaki et al 2013). In addition, paratuberculosis has already been detected in the southern (Fiss et al 2015) and metropolitan (Driemeier et al 1999) regions of RS. However, these reports are from herds where the disease was only initially detected by observing clinical cases.…”

Section: Introductionmentioning

confidence: 99%

Detection of paratuberculosis in dairy cows from southern Brazil

et al. 2023

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Bovine paratuberculosis causes chronic, incurable diarrhea and weight loss, resulting in decreased cattle production. The disease is caused by Mycobacterium avium subsp. paratuberculosis (MAP), an obligate intracellular mycobactin-dependent mycobacterium that replicates slowly in the host and has heightened environmental resistance. In countries where the disease is found and the damage has been quantified, direct and indirect economic losses are extremely high. Local epidemiological data is of paramount importance for the implementation of control programs. Our objective was to verify whether paratuberculosis is present in commercial dairy herds in different mesoregions of RS. Therefore, a prospective, cross-sectional and observational study was performed on dairy cattle from five mesoregions of the RS state, Brazil. Milk samples taken from individual cows on commercial farms were tested using indirect ELISA tests and classified as negative, suspicious, or positive. In herds containing at least one positive cow, we conducted convenience sampling of feces directly from the rectal ampulla to identify MAP through PCR. Of the 362 cows tested, 20 were seroreactive for paratuberculosis from two mesoregions. The PCR tests were all positive; cows with a negative ELISA and positive PCR results probably indicate that the MAP was ingested and eliminated without causing infection. We found that paratuberculosis is likely endemic in the northwest and northeast mesoregions.

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“…The absence of clinical signs does not discard the presence of infection in the herd, in these cases it is necessary to combine diagnostic techniques to confirm infected animals [18]. The clinical manifestations are generally observed after 3 years of age, although MAP can remain incubated for a period that ranges from 6 months up to 15 years, resulting in late clinical signs [13,15].…”

Section: Discussionmentioning

confidence: 99%

Intercurrence of Paratuberculosis in Intradermal Tuberculin Test Reactive Cattle

Souza

Soares

Medeiros-Ronchi

et al. 2020

Acta Scientiae. Vet.

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Background: Bovine tuberculosis control programs are based on a standard diagnostic method, the intradermal test with purified protein derivatives, which is used to identify and eliminate diseased animals. Currently none of the tests available allow complete differentiation between infected and uninfected animals. The main limitations of the tests available are related to diagnostic sensitivity and specificity, which results in false positive reactions due to the existence of cross infections, and also false negative, inherent to the state of energy of some animals. The aim of this work was to study the intercurrence of paratuberculosis in tuberculosis reactive cattle by the comparative cervical test.Materials, Methods & Results: Three hundred and thirty four cattle were evaluated using the comparative cervical test (CCT) and serology for tuberculosis (TB) and paratuberculosis (PTB) ELISA IDEXX®. All of the animals testing positive by CCT were euthanized and necropsied. Fragments of lymph node, lung and intestine were collected and analyzed using histopathological techniques, with staining by Hematoxylin-Eosin (HE). Samples of lung and lymph nodes (retropharyngeal, submandibular, cervical and mediastinal) of the animals testing positive by CCT were evaluated using qPRC for M. bovis, and intestinal and mesenteric lymph nodes using PCR for PTB. Of the 334 cattle evaluated using the comparative cervical test, 16 were considered positive. No lesions suggestive of tuberculosis were found in the macroscopic inspection of the carcasses. The most evident anatomical and pathological finding was a thickening of intestinal mucosa, found in 12 of the 16 cattle submitted to necropsy. No microscopic lesions suggestive of TB were identified nor was the presence of M. bovis detected by qPCR. The main histopathological findings were observed in the small intestine and mesenteric lymph nodes and identified as enteritis, lymphangitis, lymphangiectasia and granulomatous lymphadenitis. In the intestine the changes are characterized by dilated and inflamed lymphatic vessels and intense inflammatory infiltrate on the mucosa and submucosa. Of the 334 serum samples evaluated, the M. bovis ELISA Antibody Test (IDEXX®) identified 17 positive animals. All the cattle considered positive by M. bovis ELISA were considered negative by CCT. In the samples from nine animals (9/16), DNA from M. avium subsp. paratuberculosis (MAP) was identified and in twelve carcasses (12/16) lesions characteristic of PTB were found, which were subsequently confirmed by histopathological techniques. In another nine animals of the herd anti-MAP antibodies were detected. None of those that tested positive by PTB ELISA were reactive by CCT.Discussion: Animals considered positive by TB ELISA that were not positive in the intradermal test does not mischaracterize the clinical picture of the disease. Considering the inverse relationship between cell-mediated and humoral responses to M. bovis, the intradermal test and the serological tests are designed to measure different immunological responses, which develop during different stages of infection. The progress of the cellular immunological response to humoral immunity occurs in the most advanced stages of tuberculosis. Of the 16 cattle considered positive by CCT, 12 animals presented macroscopic and histological lesions suggestive of PTB and DNA from MAP was detected in nine. Although it is the official test for the control of TB in different countries, the intradermal test with PPD has presented limitations, primarily related to specificity. M. avium subsp. Paratuberculosis is considered the main cause of false positive reactions in the intradermal test. The PPD bacterial extract is a complex mixture of proteins, lipids, sugars and nucleic acids, and many of these components are also shared by numerous species of mycobacteria (tuberculous or not).

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Paratuberculose em bovinos de corte na região Sul do Rio Grande do Sul

Cited by 9 publications

References 23 publications

Clinical bovine paratuberculosis - first report in the state of Pará

Clinical bovine paratuberculosis - first report in the state of Pará

Detection of paratuberculosis in dairy cows from southern Brazil

Intercurrence of Paratuberculosis in Intradermal Tuberculin Test Reactive Cattle

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