Parentage testing with 14 STR loci and population data for 5 STRs in the Slovenian population

Abstract

In order to apply a set of 14 short tandem repeat (STR) loci in parentage testing, we performed a population genetic study on a sample of 260 unrelated people from the Slovenian population. Genotypes for the 14 STRs were determined using three multiplex polymerase chain reactions (PCR) and automated fluorescent detection. The allele frequencies of the STR loci D5S818, D13S317, D7S820, D8S1179 and D18S51 showed no deviation from the Hardy-Weinberg equilibrium and agreed well with other Caucasian populations. We resolved a series of 181 parentage disputes of which 29 were exclusions. In all cases, evidence for exclusion was obtained by at least 4 informative STRs out of the 14 loci analysed. The 14 loci combined comprise a highly discriminating test suitable for paternity and identity testing in the Slovenian population, with an average estimated mutation rate of 1.2x10(-3), a combined calculated power of exclusion of 99.99974% and paternity index (PI) value of >10(6) in 72% of the inclusion cases and >10(5) in 91% of the inclusion cases.

Allele frequencies for 15 short tandem repeat loci in a representative sample of Bosnians and Herzegovinians

“…In comparison of B&H and southern Croatian data [5] no significant difference was found at any individual locus. The same statistical parameters were obtained in comparison with pooled Slovenian data 1 [6][7][8][9]. Significant differences (P < 0.05 with Bonferroni's correction) were found at FGA locus in comparative analysis of B&H and pooled Austrian data 1 [10][11][12][13][14][15][16][17][18][19][20][21][22] at Penta E locus in comparison with PP16 examined Caucasian populations.…”

Slovenian population data for five new European Standard Set Short tandem repeat loci and SE33 locus

“…Slovenian allele frequencies differed only from Spanish (17) and Italian population (16), where deviations were observed only for D2S441 and D12S391. Deviations of Slovenian allele frequency distribution from Spanish and Italian population were also observed in previous population data studies (6,7) on the loci TH01, D7S820, and D8S1179. Since the sample size for SE33 locus was small we expected some deviations of allele frequency distribution from Austrian (15), Italian (16), German (18), and Spanish (19) populations, but none were observed.…”

“…These kits also include the AmpFlSTR NGM TM PCR Amplification Kit (Applied Biosystems, Foster City, CA, USA) and the PowerPlex ESX 17 System (Promega, Madison, WI, USA), which we used for our population study. Allele frequencies for autosomal STRs (6,7), Y-chromosomal STRs (8), and mitochondrial DNA (9) were already determined for Slovenian population and the aim of this study was to apply new genetic markers in routine forensic casework to achieve higher evidential value of STR typing and to increase the number of short STR loci, which are better preserved in degraded samples. Some European population studies have already investigated the new ESS loci (D10S1248, D22S1045, D2S441, D1S1656, D12S391) and we compared Slovenian allele frequencies with them (10-17).…”

Analysis of disputed single-parent/child and sibling relationships using 16 STR loci

“…1,14,15,16,17]. These studies all find that STR multiplexes have a high power of discrimination for paternity testing.…”