Paroxysmal nocturnal hemoglobinuria clones are not present in HIV positive patients

Bieliauskas, Shannon; Fine, Nancy; Douglas-Nikitin, Vonda; Blenc, Ann Marie

doi:10.1002/cyto.b.20559

Cited by 1 publication

(1 citation statement)

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“…PNH diagnosis was performed by FC on PB as elsewhere described [9,20]. Data acquisition was carried out by a FACS Canto II (BD) flow cytometer; for data analysis, Diva Software (BD) was used.…”

Section: Pnh Fc Analysis On Pbmentioning

confidence: 99%

A systematic analysis of bone marrow cells by flow cytometry defines a specific phenotypic profile beyond GPI deficiency in paroxysmal nocturnal hemoglobinuria

Mannelli¹,

Bencini²,

Peruzzi³

et al. 2012

Cytometry Part B Clinical

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Background. Paroxysmal nocturnal hemoglobinuria (PNH) is a unique disorder caused by a PIG-Methods. In the present study, we have analyzed systematically FC data resulting from the study of BM cells from patients with PNH and MDS.Results. Our data demonstrated abnormalities in PNH beyond the deficiency of glycosylphosphatidylinositol-linked proteins and the application of a systematic approach allowed us to separate effectively MDS and PNH in a cluster analysis and to highlight disease-specific abnormalities. Indeed, the parallel evaluation of some key parameters, i.e. patterns of expression of CD45 and CD10, provided information with practical diagnostic usefulness in the distinction between PNH and MDS. Moreover, the hypoexpression of CD36 that we observed on monocytes might be related to the thrombotic tendency in PNH.Conclusions. We investigated systematically the phenotypic profile of BM cells from patients with PNH; our data provide useful antigenic patterns to solve between PNH and MDS, sometimes morphologically overlapping. Moreover, some PNH-related phenotypic changes might be involved in the physiopathology of the disease and further studies addressing this issue are warranted. V C 2012 International Clinical Cytometry Society

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Section: Pnh Fc Analysis On Pbmentioning

confidence: 99%