Part B. Retention parameters in gas chromatograpy

Abstract

Republication or reproduction of this report or its storage and/or dissemination by electronic means is permitted without the need for formal IUPAC permission on condition that an acknowledgment, with full reference to the source along with use of the copyright symbol ©, the name IUPAC, and the year of publication, are prominently visible. Publication of a translation into another language is subject to the additional condition of prior approval from the relevant IUPAC National Adhering Organization. CONTENTS 1.© 2001 IUPAC, Pure and Applied Chemistry 73, 969-992 Retention parameters in chromatography 971Unauthenticated Download Date | 6/20/16 5:08 PM Retention volume, adjusted retention volume, and hold-up volumeThe volume of mobile phase that leaves the column, from the moment of the entrance of the analyte into the chromatographic phase system (the initiation time) to the moment in which the analyte leaves, is called the "retention volume" of the analyte. This description does not correspond to the definition of "total retention volume" given in the IUPAC document [1] (NC 3.7.05). The difference will be shown J. A. GARCÍA DOMÍNGUEZ AND J. C. DÍEZ-MASA © 2001 IUPAC, Pure and Applied Chemistry 73, 969-992 972

Countercurrent chromatography in analytical chemistry (IUPAC Technical Report)

“…When the nature of the organic substances to be separated is known, one may find a suitable solvent system by searching the literature for solvent systems that have been successfully applied to similar compounds [2][3][4][5][6][7][8][9][10]. In case of organic-aqueous two-phase systems, the organic phase consists of one solvent or of a mixture of different solvents.…”

Countercurrent Chromatography in Analytical Chemistry (IUPAC Technical Report)

“…The use of silica as the backbone material for stationary phase in high-performance liquid chromatography (HPLC) limits the mobile-phase pH to the 2-8 range. Acidic mobile phases could cleave the organic bonded moiety; basic ones could dissolve the silica, although modern and expensive HPLC stationary phases can work with pH 1 and/or pH 10 mobile phases [10]. In contrast, if the solvents used to make the biphasic liquid system are not sensitive to pH, there are no additional pH problems as the material most frequently used in CCC columns is Teflon.…”

Biopartitioning micellar chromatography: An alternative high-throughput method for assessing the ecotoxicity of anilines and phenols

“…The retention factor of reference compounds were obtained according to the IUPAC approach [17], based on the extracolumn time, t ext , correction:…”