Partial Characterization of an Anti-Listerial Bacteriocin from
                        Enterococcus faecium CJNU 2524

Yang, Jung-Mo; Moon, Gi-Seong

doi:10.5851/kosfa.2020.e98

Cited by 9 publications

(8 citation statements)

References 29 publications

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“…faecium strains that have already been characterized [50]. We further examined the kinetics of bacteriocin production, and it was shown that the kinetic of bacteriocin production by BGZLM1-5 is in accordance with previously published data showing the more rapid production of enterocins in the initial stages of cell growth, and the decrease in enterocins production with increasing incubation time and cell density in the medium [51]. These authors assumed that such kinetics could be a part of several regulated mechanisms, such as a quorum-sensing mechanism or an induction factor, a histidine kinase.…”

Section: Discussionsupporting

confidence: 83%

Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model

Popović,

Stevanović,

Radojević

et al. 2023

Microorganisms

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This study aimed to explore the probiogenomic characteristics of artisanal bacteriocin-producing Enterococcus faecium BGZLM1-5 and its potential application in reducing Listeria monocytogenes in a milk model. The BGZLM1-5 strain was isolated from raw cow’s milk from households in the Zlatar Mountain region. The whole genome sequencing approach and bioinformatics analyses reveal that the strain BGZLM1-5 is non-pathogenic to humans. Bacteriocin-containing supernatant was thermally stable and antimicrobial activity retained 75% of the initial activity compared with that of the control after treatment at 90 °C for 30 min. Antimicrobial activity maintained relative stability at pH 3–11 and retained 62.5% of the initial activity compared with that of the control after treatment at pH 1, 2, and 12. The highest activity of the partially purified bacteriocin was obtained after precipitation at 40% saturation with ammonium sulfate and further purification by mixing with chloroform. Applying 3% and 5% (v/v) of the bacteriocin-containing supernatant and 0.5% (v/v) of the partially purified bacteriocin decreased the viable number of L. monocytogenes ATCC19111 after three days of milk storage by 23.5%, 63.5%, and 58.9%, respectively.

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Section: Discussionsupporting

confidence: 83%

Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model

Popović,

Stevanović,

Radojević

et al. 2023

Microorganisms

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“…10 mg/mL of DMSO), and pure components (conc. 10 mg/mL of DMSO) including resveratrol, oxyresveratrol, and quercetin against acne-causing C. acnes [ 30 ]. C. acnes KCTC 3314 and KCTC 3320, which are strictly anaerobic bacteria, were cultured in RCM broth (BD, Sparks, MD, USA) in an anaerobic jar (Oxford, Cambridge, UK) with GasPaK™ (BD).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds

et al. 2022

Self Cite

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The root of Smilax china L. is used in traditional Korean medicine. We found that the Smilax china L. root extract has strong antimicrobial activity against two Cutibacterium acnes strains (KCTC 3314 and KCTC 3320). The aim of this study was to identify the beneficial properties of Smilax china L. extracts for their potential use as active ingredients in cosmetics for the treatment of human skin acne. The high-performance liquid chromatography (HPLC) and liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC/QTOF/MS) methods were used to obtain the profile of secondary metabolites from the ethyl acetate-soluble fraction of the crude extract. Agar diffusion and resazurin-based broth microdilution assays were used to evaluate antimicrobial activity and minimum inhibitory concentrations (MIC), respectively. Among the 24 metabolites, quercetin, resveratrol, and oxyresveratrol were the most potent compounds against Cutibacterium acnes. Minimum inhibitory concentrations of quercetin, resveratrol, and oxyresveratrol were 31.25, 125, and 250 μg/mL, respectively.

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“…Bacteriocins with antilisterial activity have been isolated from diverse lactic acid bacteria (LAB) such as Enterococcus spp., Lactobacillus spp., Leuconostoc spp., and Weissella spp. (Dong et al, 2022;Ghalfi et al, 2006;Todorov, 2010;Woo et al, 2021;Yang and Moon, 2021). Among them, nisin, an antibiotic bacteriocin produced by Lactococcus lactis, is the only bacteriocin used in the food industry in many countries to improve food safety and extend the shelf life of foods (Churklam et al, 2020;van Kuijk et al, 2012).…”

Section: Articlementioning

confidence: 99%

“…The purified r-agilicin C7 was incubated for 2 h at 37℃ after adding proteinase-K, α-amylase, α-chymotrypsin, pepsin, and lipase at a 5 mg/mL concentration, respectively (Yang and Moon, 2021). All the enzymes were purchased from Sigma-Aldrich.…”

Section: Effect Of Enzymes Temperature Ph and Solvents On Anti-lister...mentioning

confidence: 99%

Characterization of Novel Amylase-Sensitive, Anti-Listerial Class IId Bacteriocin, Agilicin C7 Produced by Ligilactobacillus agilis C7

Yoo¹,

Song²,

Vasquez³

et al. 2023

Food Sci Anim Resour

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Among various biological agents, bacteriocins are important candidates to control Listeria monocytogenes which is a foodborne pathogen. In this study, a novel bacteriocin, named agilicin C7, was isolated from Ligilactobacillus agilis C7 showing inhibitory activity against L. monocytogenes. Agilicin C7 biosynthesis gene was characterized by bioinformatics analyses and heterologously expressed in Escherichia coli for further study. The anti-listeria activity of recombinant agilicin C7 (r-agilicin C7) was lost by proteases and α-amylase, suggesting that agilicin C7 is a glycoprotein. r-Agilicin C7 has wide pH and thermal stability and is also stable in various organic solvents. It destroyed L. monocytogenes by damaging the integrity of the cell envelope. These properties of r-agilicin C7 indicate that agilicin C7 is a novel amylase-sensitive anti-listerial Class IId bacteriocin. Physicochemical stability and inhibitory activity against L. monocytogenes of r-agilicin C7 suggest that it can be applied to control L. monocytogenes in the food industry, including dairy and meat products.

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Partial Characterization of an Anti-Listerial Bacteriocin from Enterococcus faecium CJNU 2524

Cited by 9 publications

References 29 publications

Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model

Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model

Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds

Characterization of Novel Amylase-Sensitive, Anti-Listerial Class IId Bacteriocin, Agilicin C7 Produced by Ligilactobacillus agilis C7

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