Partial characterization of esterases from Fusarium culmorum grown in media containing di (2-ethyl hexyl phthalate) in solid-state and submerged fermentation

Las enzimas esterasas son de gran importancia en el área biotecnológica debido a las reacciones de interesterificación, transesterificación y esterificación que llevan a cabo. Las esterasas microbianas pueden ser secretadas por hongos filamentosos, levaduras o bacterias. En base a las características de cada enzima, éstas se pueden emplear en las industrias del vino y de lácteos, en la degradación de sustratos complejos, en biorremediación de sitios contaminados, entre otras aplicaciones. La enzima de interés debe ser caracterizada para que pueda ser producida a nivel industrial. El proceso de producción industrial de las enzimas se lleva a cabo principalmente en fermentación líquida. En general, este proceso consiste en una serie de pasos que inician con la inoculación del organismo, mismo que debe crecer en condiciones óptimas, posteriormente se lleva a cabo el pretratamiento de la enzima de interés, seguido del concentrado de ésta por medio de filtración para eliminar el excedente de agua y finalmente se realiza la purificación del producto.

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Growth and esterase activity of Fusarium culmorum grown in di(2- ethyl hexyl) phthalate in liquid fermentation

Ríos-González¹,

González-Marquez

Sánchez

2019

Mexjbiotechnol

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Di (2-ethyl hexyl) phthalate (DEHP) is a plasticizer present in various products, mainly those made with PVC. This phthalate has attracted attention due to its important participation in the contamination of the environment. It has been reported as an endocrine-disrupting compound in mammals. Fusarium culmorum is a phytopathogenic fungus able to degrade DEHP, because it produces esterases, which are enzymes capable to break down ester bonds present in the structure of phthalates. In this research, growth, protein content and esterases activity by biochemical tests and polyacrylamide gel electrophoresis were characterized for F. culmorum grown in DEHP-supplemented (100 and 1500 mg/L) media as the only carbon source in liquid fermentation. F. culmorum showed higher biomass production and esterase activity in medium supplemented with 1500 mg of DEHP/L. Zymography revealed that bands with esterase activity were observed after 24 h and 48 h in media supplemented with 1500 and 100 mg of DEHP/L, respectively. It was shown that DEHP is an inducer of esterases and that this compound was used as carbon and energy sources by F. culmorum. This fungus can secrete specific esterase to breakdown high concentrations of DEHP, being a promising organism for bioremediation of DEHP-polluted environments in both aquatic and terrestrial ecosystems.

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Partial characterization of esterases from Fusarium culmorum grown in media containing di (2-ethyl hexyl phthalate) in solid-state and submerged fermentation

Cited by 5 publications

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Whole Organism Biotechnology

Whole Organism Biotechnology

Producción de esterasas por microorganismos: importancia y aplicación industrial

Growth and esterase activity of Fusarium culmorum grown in di(2- ethyl hexyl) phthalate in liquid fermentation

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