Partial dehydration and cryopreservation of <i>Citrus</i> seeds

Graiver, Natalia Gisel; Califano, Alicia Noemí; Zaritzky, Noemí Elisabet

doi:10.1002/jsfa.4427

Cited by 13 publications

(11 citation statements)

References 39 publications

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“…The capability of oily seeds to survive at low temperatures is strictly connected to avoidance of intracellular ice formation (Chien and Chen, 2008). In this study results are similar to the results of Graiver et al (2011) for citrus species (C. reticulata, C. sinensis, and C. paradisi). They observed that seed MC under the high-moisture freezing limit caused a decrease in seed survival after liquid nitrogen exposure.…”

Section: Cryopreservation Of Citrus Seedssupporting

confidence: 89%

“…The same results for oil-rich seeds considered as intermediate were obtained from citrus (Graiver et al, 2011) and coffee (Dussert et al, 2001) species for liquid nitrogen at higher MCs. The capability of oily seeds to survive at low temperatures is strictly connected to avoidance of intracellular ice formation (Chien and Chen, 2008).…”

Section: Cryopreservation Of Citrus Seedssupporting

confidence: 73%

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Cryopreservation of citrus seed via dehydration followedby immersion in liquid nitrogen

Kaya¹,

Souza²,

Gökdoğan³

et al. 2017

Turk J Biol

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An important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation. Cryopreservation refers to the storage of plant material at ultralow temperatures in liquid nitrogen. A procedure for cryopreservation of polyembryonic seeds was improved for selected citrus cultivars from Turkey. Seed dehydration was performed at different exposure times, in sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. × Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck. The postcryopreservation germinability ranged from 73.3% (Poncirus trifoliata Raf. × C. sinensis Osb. and Fortunella margarita (Lour.) Swingle) to 93.3% (C. jambhiri Lush.). Dehydration was beneficial for germination of seeds from all of the tested citrus species after the liquid nitrogen exposure. Seedlings derived from cryopreserved seeds had well-formed shoots and roots and were easily acclimated to greenhouse conditions.

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Section: Cryopreservation Of Citrus Seedssupporting

confidence: 89%

Section: Cryopreservation Of Citrus Seedssupporting

confidence: 73%

Cryopreservation of citrus seed via dehydration followedby immersion in liquid nitrogen

Kaya¹,

Souza²,

Gökdoğan³

et al. 2017

Turk J Biol

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“…However, both RP and NS in the humidity of 15% wb are very low in relation to those of 20% wb. Graiver et al (2011) stated that drying Citrus seeds, also sensitive to desiccation, to a limiting water content that prevents intracellular ice formation is essential for their survival to cryopreservation.…”

Section: Experimental Designmentioning

confidence: 99%

Exploratory studies for cryopreservation of Coffea arabica L. seeds

et al. 2017

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-Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests.Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.Index terms: silica gel, water content, slow cooling, liquid nitrogen, coffee.Estudos exploratórios para a criopreservação de sementes de Coffea arabica L.RESUMO -A criopreservação é uma opção viável para a conservação de germoplasma de café. Entretanto, para que essa técnica apresente total sucesso é de fundamental importância realizar pesquisas que garantam a manutenção da integridade celular antes e após a imersão em NL. Portanto, o objetivo neste estudo foi investigar o teor de água, velocidade e temperatura final de resfriamento mais adequados para criopreservar sementes de Coffea arabica L. As sementes foram secadas em sílica gel até teores de água de 5, 10, 15, 20, 30 e 40% bu, submetidas a tratamentos de resfriamento lento nas velocidades -1, -3 e -5 ºC min -1 até às temperaturas finais de -40, -50 e -60 ºC e diretamente imersas em NL. Após armazenamento as sementes foram reaquecidas por dois minutos à 40 ºC. A sobrevivência e a viabilidade de sementes e embriões foram avaliadas por meio do teste de tetrazólio e de germinação. Por meio do teste de tetrazólio, observa-se que embriões excisados das sementes criopreservadas são menos sensíveis à criopreservação quando comparados às sementes inteiras. O teor de água de 20% bu permite maior sobrevivência das sementes de Coffea arabica L., bem como de embriões zigóticos após a criopreservação, dependendo das velocidades e temperaturas finais de pré-resfriamento.Termos para indexação: sílica gel, teor de água, resfriamento lento, nitrogênio líquido, café.

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“…This method is commonly utilized for the calculation of water/moisture in experiments with oilrich seeds: i.e. previous work on Prunus avium seeds [25], Camellia sinensis seeds [30], Corylus avellana seeds [31], Citrus seeds: C. sinensis (sweet orange), C. paradisi (grapefruit), C. reticulata (mandarin) [32] or embryonic axes i.e. : Carica papaya, Passiflora edulis and Laurus nobilis [33].…”

Section: Moisture Content Of the Seedsmentioning

confidence: 99%

“…: citrus species (C. grandis, C. madurensis, C. reticulate,C. sinensis, C. paradisi, C. reticulate) [32,47], and coffee species (C. arabica, C. eugenioides, C. pseudozanguebariae, C. racemosa, C. sessiliflora) [48].…”

Section: Sensitivity Of Mazzard Cherry Seeds To Desiccationmentioning

confidence: 99%

A new insight in desiccation tolerance and cryopreservation of mazzard cherry (Prunus avium L.) seeds

2015

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A variable response of Prunus avium L. seeds to desiccation and storage in liquid nitrogen (LN) has been reported in the literature. The majority of these experiments were conducted on initially dried seeds. The desiccation and LN exposure tolerance of fresh P. avium seeds is unknown. In the present study, fresh seeds were used to determine seed response to desiccation and cryopreservation. Desiccation of seeds from a moisture content (MC) of 19.7-20.2% to 10.1-10.9% or 07.6-8.5% reduced seedling emergence from approximately 73 to 19 and 16% for first provenance; and from approximately 89 to 10-12% for second provenance of seeds. After exposure to LN, seeds had the highest seedling emergence when seed MC was the highest (19.7 and 20.2%, respectively) prior to cryostorage. Results indicated that P. avium seeds should be classified as intermediate. For cryopreservation in seed banks, we recommend that seeds be dried directly after extraction from fruits in the range of 16.8-20.2% of MC (0.21-0.25 g·g

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Partial dehydration and cryopreservation of Citrus seeds

Abstract: The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen WC in the tissue, confirming that seed survival strictly depended on avoidance of intracellular ice formation.

Cited by 13 publications

References 39 publications

Cryopreservation of citrus seed via dehydration followedby immersion in liquid nitrogen

Cryopreservation of citrus seed via dehydration followedby immersion in liquid nitrogen

Exploratory studies for cryopreservation of Coffea arabica L. seeds

A new insight in desiccation tolerance and cryopreservation of mazzard cherry (Prunus avium L.) seeds

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