Partial nephrectomy in mice with milliwatt carbon dioxide laser and its influence on experimental metastasis

Ammirati, Mario; Rao, Leela N.; Murthy, M. Satya; Buchmann, Tamara; Goldschmidt, Robert; Scanlon, Edward F.

doi:10.1002/jso.2930410305

Cited by 18 publications

(9 citation statements)

References 15 publications

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“…In addition to this difficulty, even those xenografts that are derived from metastatic tumors fail to metastasize in the host animals. with l0 s cells develop tumors in these organs [10][11][12]. Despite this aggressiveness, TA3Ha cells rarely implant and grow in the kidney, liver, spleen, cecum, or the bones [7,8].…”

Section: Introductionmentioning

confidence: 99%

Growth and metastasis of human breast cancers in athymic nude mice

et al. 1995

Clin Exp Metast

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To evaluate critically the merit of utilizing a wound model for growing human tumors, a series of increasingly difficult human tumor types were tested for growth at sites of trauma in athymic nude mice. In vitro tumor lines as well as fresh tumors from the breast, colon, rectum, lung, and a metastasis from an unknown primary were intraperitoneally injected into mice subjected to intra-abdominal organ injury. Successful xenografts were obtained from nine of 10 cell lines and 14 of 24 fresh tumors. The latter included five of six (83%) colon cancers, one lung tumor, metastatic tumor of unknown primary, three of four (75%) metastatic breast cancers and four of six (67%) estrogen receptor (ER)-negative breast primary tumors. Six ER-positive breast tumors tested failed to grow in mice without estrogen supplementation. Xenografts from two breast, two colon and the lung cancers formed spontaneous metastases and all xenografts tested were able to yield serial transplants in the surgical wound model. Histologically, all xenografts and their metastases were identical to their respective donor tumors. Transplantability in mice without exogenous estrogen supplementation was linked to the absence of estrogen and progesterone receptors in breast tumors. Transplantability of the cell lines was associated with the expression of cell surface receptors for fibronectin and hyaluronic acid. Receptors for other extracellular matrix components, namely, laminin, vitronectin, collagen, fibrinogen or von Willebrand factor were not associated with transplantability. These results demonstrate that a large proportion of human tumors, including the breast tumors, can be successfully xenografted into athymic mice by providing them with a healing wound environment, and that such xenografts grown at ectopic sites exhibit metastatic ability.

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Section: Introductionmentioning

confidence: 99%

Growth and metastasis of human breast cancers in athymic nude mice

et al. 1995

Clin Exp Metast

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“…injected syngeneic TA3Ha tumor cells selectively implant at sites of hepatic trauma induced by electrocautery [6,7]. Tumor implantation has also been noted in mice following surgery of the kidney, skin, caecum, spleen, or the pelvic bone [5][6][7]; in the absence of trauma, these sites are rarely affected by metastases [18,19]. In all the sites so far examined, the highest rate of tumor implantation in the wounds was seen when tumor cells were injected immediately after surgery [5][6][7]; as the interval between surgery and tumor cell inoculation increased the frequency of tumor implantation decreased.…”

Section: Discussionmentioning

confidence: 99%

“…Several investigators have reported that injury resulting from surgical [1][2][3][4][5][6][7][8] or mechanical trauma [9,10], exposure to radiation and chemicals [11][12][13][14][15], and treatment with anticancer drugs [16,17] favors tumor implantation in healing wounds. Our focus has been on implantation of i.v.…”

Section: Introductionmentioning

confidence: 99%

“…Surgical trauma to organs such as the liver, kidney, cecum, spleen, and the skin of mice renders these tissues susceptible to metastasis by TA3Ha mouse mammary carcinoma cells [5][6][7]; when uninjured these same tissues are uncommon sites of metastasis [18,19]. The frequency of tumor implantation in these injured sites decreases as the interval between trauma and tumor cell presentation is increased [5,6], suggesting that early events in wound healing are important for tumor cell implantation.…”

Section: Introductionmentioning

confidence: 99%

“…The frequency of tumor implantation in these injured sites decreases as the interval between trauma and tumor cell presentation is increased [5,6], suggesting that early events in wound healing are important for tumor cell implantation. Tumor implantation is also reduced when mice are treated with certain plasminogen activators [20], suggesting that tumor cells interact with proteins in the wound that are susceptible to degradation by plasmin.…”

Section: Introductionmentioning

confidence: 99%

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The role of fibronectin in tumor implantation at surgical sites

et al. 1993

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Fibronectins are a family of glycoproteins with modular functional domains. They mediate cell-cell and cell-matrix interactions which are important in embryogenesis, wound healing, metastasis and other processes. We present data on the influence of fibronectin on wound implantation of a murine mammary carcinoma line, TA3Ha. Fibronectin used in these studies was derived from bovine plasma, human serum, human foreskin fibroblasts, and mouse embryo cultures. TA3Ha cells rarely form tumors in the liver of syngeneic mice when injected intravenously but after hepatic wedge resection, 45% (107/240) of the mice develop tumors in the hepatic wound. Wound implantation is markedly reduced when the cells are pre-exposed to 200 micrograms/ml bovine plasma fibronectin (13%, P = 0.007), human serum fibronectin (0%, P = 0.02), human cellular fibronectin (0%, P = 0.02), or mouse cellular fibronectin (0%, P = 0.04). Lung colonization is also reduced by these fibronectins. These effects are not due to a cytotoxic action of fibronectin, since intraperitoneally injected fibronectin-treated cells form ascites tumor as effectively as do control untreated cells. Local application of a solution containing 0.25 mg/ml mouse cellular fibronectin to the hepatic wound reduces the frequency of tumor implantation from 45% to 5% (1/21, P = 0.001). No tumor implantation inhibition is seen when only suspending medium or albumin in suspending medium is used. The mechanism by which topical application of fibronectin reduces hepatic wound implantation of tumor cells is unclear, but this finding raises an exciting possibility of preventing local recurrence of cancer.

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