Partial Regeneration/Proliferation of the .BETA.-Cells in the Islets of Langerhans by Nigella sativa L. in Streptozotocin-Induced Diabetic Rats

Kanter, Mehmet; Meral, İsmail; Yener, Zabit; Özbek, Hanefí; Demır, Halit

doi:10.1620/tjem.201.213

Cited by 129 publications

(93 citation statements)

References 8 publications

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“…However, our findings were in accordance with that of Kanter et al [34] who found that diabetic rats treated with NSO showed improvement after 10 days treatment compared to the diabetic rats.…”

Section: Discussionsupporting

confidence: 93%

The Antidiabetic Effect of Mesenchymal Stem Cells vs. Nigella sativa Oil on Streptozotocin Induced Type 1 Diabetic Rats

Mohamed¹,

Ali²,

Hosny³

2015

J Cell Sci Ther

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Introduction: Diabetes is a chronic metabolic disorder that has no definitive cure till present. Currently; regenerative medicine using Mesenchymal Stem Cells (MSCs) offers promising treatment. Meanwhile, Nigella Sativa Oil (NSO) shows effectiveness for medication of various illnesses.Objective: This study compares the antidiabetic effect of MSCs vs. NSO on streptozotocin induced type 1 diabetes (T1D) in rat model. Methods:Human cord blood samples were collected. Isolated mononuclear cells were cultured and incubated to isolate MSCs. For experimental animals; after induction of T1D using multiple low dose streptozotocin (MLD-STZ), diabetic rats were divided into 6 groups (n =10); diabetic group 1 and 2 (sacrificed 15 and 30 days post-induction, consequently), NSO treated groups 1 and 2 (received daily NSO I.P. injection, sacrificed 15 and 30 days postinduction, consequently), MSCs treated groups 1 and 2 (72 hours post-induction, animals were treated with twice intravenous injection of 1×106 cells/rat, 24 hours apart, without immunosuppression, sacrificed 15 and 30 days of induction, consequently).Results: Low blood glucose levels were detected in both MSCs and NSO groups, which reached near normal levels within 15 days. This was accompanied by improvement of the histopathological changes and increase in the immunreactive insulin secreting cells, obviously detected after 30 days.

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Section: Discussionsupporting

confidence: 93%

The Antidiabetic Effect of Mesenchymal Stem Cells vs. Nigella sativa Oil on Streptozotocin Induced Type 1 Diabetic Rats

Mohamed¹,

Ali²,

Hosny³

2015

J Cell Sci Ther

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“…The animals became diabetic within 24 hours after the administration of STZ. Intraperitoneal injection of 0.20 ml/kg volatile oil of N. sativa seeds for 30 days in such rats, caused gradual partial regeneration/proliferation of pancreatic beta-cells, decrease in the elevated serum glucose and increase of the lowered serum insulin concentrations [40]. Similar effects were observed in STZ induced diabetic rats with N. sativa (300 -400 mg/kg body weight) or thymoquinone (50 mg/kg body weight) once a day orally for 12 weeks [41,42].…”

Section: Antidiabetic Activity In Stz Induced Diabetic Ratsmentioning

confidence: 99%

Antidiabetic Properties of a Spice Plant Nigella sativa

Mathur

2011

J Endocrinol Metab

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Seeds of Nigella sativa (black cumin/kalonji) used in pickles as spice, have also been traditionally used in treatment of many diseases including diabetes and hypertension. Among many activities exhibited by N. sativa and its constituents in animal experiments, antidiabetic property is most important. Thymoquinone (TQ), a volatile oil, is one of its active constituents but antidiabetic activity has also been shown by its aqueous extract and defatted extract. N. sativa may be beneficial in diabetic individuals and those with glucose intolerance as it reduces appetite, glucose absorption in intestine, hepatic gluconeogenesis, blood glucose level, cholesterol, triglycerides, body weight and simulates glucose induced secretion of insulin from beta-cells in pancreas; improves glucose tolerance as efficiently as metformin; yet it has not shown significant adverse effects and has very low toxicity. In streptozotocin (STZ) induced diabetic rats it causes gradual partial regeneration of pancreatic beta-cells, increases the lowered serum insulin concentrations and decreases the elevated serum glucose. N. sativa has antioxidant activity and protective role of TQ against development of type I diabetes may be via NO inhibitory pathway. It also exerts an insulin-sensitizing action in hepatocytes. Seeds of N. sativa have been safely consumed by human patients in many clinical trials which however were not aimed to assess its antidiabetic activity. In future clinical studies may show potential of N. sativa, its constituents or their synthetic analogues, in prevention and control of diabetes.

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“…STZ treated rats with blood glucose level ≥18 mmol/litre were considered to be diabetic and used in this study [15].…”

Section: For Biochemical Studymentioning

confidence: 99%

Effect of Quercetin on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study

Rifaai¹,

El‐Tahawy²,

Saber³

et al. 2012

J Diabetes Metab

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Background: Insulin dependent diabetes mellitus is a chronic metabolic disease. Many drugs used in its treatment which have a number of serious adverse effects. Natural agent as quercetin (QCT) has been suggested in the alternative medicine for treatment of diabetes mellitus. Aim of the work:To evaluate the effect of QCT on the histological changes which occur in the islet of Langerhans of the streptozotocin (STZ)-induced diabetic rats and the possible mechanisms through which QCT produces its protective effect. Materials and methods:Fourty five male albino rats were divided into 3 groups: Group I, control group; 15 rats received single intraperitoneal injection of citrate buffer saline. Group II, diabetic group; 15 rats received single intraperitoneal injection of STZ. Group III; QCT-treated group, 15 rats received daily intraperitoneal injections of QCT for 30 days prior to, and for 30 days after the single injection of STZ. Blood samples were taken for monitoring blood glucose levels. Pancreas was taken out and processed for light microscopic, immunocytochemical and morphometrical studies.Results: Blood glucose levels showed a significant increase in group II compared to the control, while a significant decrease was observed in groups III compared to group II (all p<0.05). In the hematoxylin-eosin stained sections, STZ administration caused marked degeneration of islet Beta cells with inflammatory cells infiltration. Using QCT; in group III, reversed most of the pancreatic morphological changes, and interestingly some islets noticed with connections to some pancreatic ducts. In chrome alum heamatoxylin stained sections, STZ administration caused a significant decrease in the number of bluish stained β cells compared to controls. While group III showed a significant increase in β cells number compared to group II (all p<0.05). Sections of animals injected with charcoal, showed many charcoal labeled macrophages in group II compared to group III, and controls. There was increased iNOS and caspase 3 immunoreactivity in islets cells of group II than in controls, and was decreased in group III. Conclusion:This study provides evidence that quercetin could exert a protective effect against β cell damage by its anti-inflammatory, anti-apoptotic, and antioxidant effects; and aids regeneration of β cells which might through stimulation of the ductal stem cells. However, further experimental studies are still needed for more details on quercetin as an adjuvant drug in management of diabetes mellitus. Journal of Diabetes and MetabolismCitation: Rifaai RA, El-Tahawy NF, Saber EA, Ahmed R (2012) Effect of Quercetin on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab 3:182.

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Partial Regeneration/Proliferation of the .BETA.-Cells in the Islets of Langerhans by Nigella sativa L. in Streptozotocin-Induced Diabetic Rats

Cited by 129 publications

References 8 publications

The Antidiabetic Effect of Mesenchymal Stem Cells vs. Nigella sativa Oil on Streptozotocin Induced Type 1 Diabetic Rats

The Antidiabetic Effect of Mesenchymal Stem Cells vs. Nigella sativa Oil on Streptozotocin Induced Type 1 Diabetic Rats

Antidiabetic Properties of a Spice Plant Nigella sativa

Effect of Quercetin on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study

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