Partial replacement of commercial soybean meal with raw, full-fat soybean meal supplemented with varying levels of protease in diets of broiler chickens

Abstract

A 3 * 3 factorial study was used to evaluate the feed intake (FI), body weight gain (BWG), and feed conversion ratio (FCR) of broilers fed on the test diets. The commercial soybean meal (SBM) was replaced by raw, full-fat soybean meal (RSBM) at 0, 10 or 20%, equivalent to 0, 30 and 60 g/kg of diet, respectively, and the microbial protease was also supplemented in diets at 0.1, 0.2 or 0.3 g/kg, equivalent to ~7500, 15000 and ~22500 PROT/kg of diet, respectively. Microbial phytase (1000 FYT/kg) was uniformly added to each diet, which was fed to six replicate groups, with nine birds per replicate. Samples of test-ingredients and test-diets, after mixing, were also subjected to chemical analysis, prior to being used, to assess the nutritional compositions, particularly anti-nutritional factors. As the result of this study, the analysed concentrations of trypsin inhibitors (TI) in the diets ranging between 1730.5 and 9913.2 TIU/g. Increasing the levels of RSBM in the diets reduced the FI, during 1 to 35 d of age. Except in the starter phase, increasing the level of RSBM had no significant effect on BWG. When protease was added to the diets, the BWG was significantly improved during the periods of 1 -10 d, 1 -24 d and 1 -35 d. Feed efficiency was decreased by increasing the level of RSBM (1-10 d). Neither increasing levels of RSBM nor protease affected the yield of any meat part at d 35. The relative weight of the small intestine at d 10 increased with rising levels of RSBM, but was reduced when protease was added to the diets. In conclusion, commercial SBM could be replaced by RSBM up to 20% in diets for broilers when the test microbial protease is supplemented.

Protease and phytase supplementation of broiler diets in which soybean meal is partially or completely replaced by raw full-fat soybean

“…Similarly, in the feeding trial (0 -25 days), the FI and BWG of birds on the PC and NC diets, which were supplemented with combined enzymes, were greatly improved, compared with the same measurements on other diets. These results in general are in line with previous reports (Malathi & Devegowda, 2001;Srinath et al, 2012;Erdaw et al, 2017a;2018) that better response were found in in vitro and in vivo experiments when combined microbial enzymes were used rather than individual application. These results suggest synergistic and combined effects of the two enzymes on ANF.…”

“…Not only were the combined microbial enzymes effective in the latter phase of this study, but supplementation of microbial protease alone into the NC diet contributed to approximately 5.3% and 22.5%, or 11.4% increase in FI, BWG and feed efficiency, respectively, compared with birds on NC between 26 and 31 days. These results in general agree with previous reports (Ao, 2011;Barletta, 2011;Erdaw et al, 2017a;2017b;2017c) that ANF, for example proteinaceous anti-nutrients and stored proteins, can be broken down by supplementing the diets or ingredients with microbial protease or phytase.…”

“…In recent studies, the authors examined the potential of improving the quality of diets containing RFSB for broiler chickens through supplementation with high-potency protease and phytase products. The enzymes reduced the negative impact of RFSB, although productivity was still poorer than that observed with a control diet that was devoid of RFSB (Erdaw et al, 2017a;2017b). In previous studies, protease alone, and especially in combination with phytase, improved the in vitro digestion of protein (Erdaw et al, 2016).…”

Supplementation of broiler diets with high levels of microbial protease and phytase enables partial replacement of commercial soybean meal with raw, full‐fat soybean

“…Although soybean is primarily grown for oil, the seed can also be fed to poultry with oil intact (full fat). However, raw, full‐fat soybean (RFSB) contains antinutritional factors (ANFs) that negatively influence the utilisation of nutrients in poultry (Erdaw, Perez‐Maldonado, Bhuiyan, & Iji, , ,b; Pettersson & Pontoppidan, ). The best‐characterised ANFs in raw soybean seed are protease inhibitors, lectins and phytate (Pettersson & Pontoppidan, ).…”

Physiological and health‐related response of broiler chickens fed diets containing raw, full‐fat soya bean meal supplemented with microbial protease

“…Both commercial SBM and supplemental dietary oil could partially be replaced by inclusion of full‐fat SBM, but meals prepared from RSBM could contain high concentrations of antinutritional factors (ANF), such as trypsin inhibitors (TI), lectin and phytic acid. The best characterized ANFs in soya beans are protease inhibitors, lectins and phytates (Erdaw, Bhuiyan, & Iji, ; Erdaw, Shubiao, Perez‐Maldonado, & Iji, ; Pettersson & Pontoppidan, ). Nitrogen retention by animals can be negatively affected by the activity of TI, which results in increased metabolic nitrogen excretion (Banaszkiewicz, ; Dourado, Pascoal, Sakomura, Costa, & Biagiot, ).…”